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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... which was followed by an overnight incubation at 4°C with N-hydroxysuccinimide ester derivatives of AF647 (Thermo Fisher Scientific) present in two-fold molar excess.
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Biochemistry 2023Quote: Porcine brain tubulin was isolated using the high-molarity PIPES procedure as described (Castoldi and Popov, 2003) and then labeled with biotin- or Dylight-405 NHS-ester (Invitrogen) as described (http://mitchison.hms.harvard.edu/files/mitchisonlab/files/labeling_tubul in_and_quantifying_labeling_stoichiometry.pdf) ...
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bioRxiv - Biophysics 2023Quote: ... 26 The αSA140C and αSA18C mutants were conjugated with AlexaFluor 647 and AlexaFluor 568 NHS-esters (Thermo Fisher Scientific, USA) targeting the cysteine residues ...
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bioRxiv - Biophysics 2023Quote: ... Cy3B and AF647 free dyes were prepared by hydrolyzing the corresponding NHS ( N-hydroxysuccimidyl) esters (PA63101, Cytiva, and A200006, Invitrogen) in 0.1 M sodium bicarbonate overnight ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: Antibody-dependent cellular phagocytosis was assessed by conjugation of concentrated RhCMV UCD52 to Alexa Fluor 647 using NHS-ester reaction (Invitrogen), which was allowed to proceed in the dark with constant agitation for 1–2 hours ...
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bioRxiv - Biophysics 2023Quote: Proteins were labelled by using the AF488-NHS ester according to the manufacturer’s protocol and purified from the excess of the dye by Zeba spin columns (Thermo Scientific), dialysis (Pur-A-Lyzer ...
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bioRxiv - Cancer Biology 2023Quote: Rhodamine tubulin was generated by labeling tubulin with X-rhodamine SE (5(and-6) carboxy-X-rhodamine succinimidyl ester) dye (ThermoFisher). Labeling stoichiometry was determined using a molar extinction coefficient of 115,000 M-1 cm-1 for tubulin and 78,000 M-1 cm-1 for X-rhodamine [58] ...
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bioRxiv - Molecular Biology 2023Quote: ... as already described [17] and reseeded onto 0.2 mm bone slices labelled with N-hydroxysuccinimide ester-activated rhodamine fluorescent dye (ThermoFisher Scientific), at a cell density of 1x105 cells/bone slice (Boneslices.com) ...
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bioRxiv - Cell Biology 2023Quote: ... as per the manufacturer’s protocol) and a fluorescent Alexa Fluor tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Cell Biology 2023Quote: HM sEV uptake by Caco-2 cells was performed after labeling EVs with carboxyfluorescein succinimidyl ester (CFSE; Thermo Fisher Scientific) [29] ...
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bioRxiv - Microbiology 2024Quote: ... 108 CFU of pneumococci were stained in 100 μl PBS containing 10 μg FITC (Thermo) or AF647-NHS ester (Invitrogen) for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2024Quote: ... we added an aliquot of barcoded reference cells stained with Alexa Fluor 647 Succinimidyl Ester (0.02 μg/μL) (ThermoFisher Scientific) to all samples ...
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bioRxiv - Biochemistry 2024Quote: NHE activity was measured fluorometrically using the intracellularly trapped pH-sensitive dye BCECF (2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester) (#B1170; Invitrogen) with the NH4Cl prepulse technique as described previously (Simonin & Fuster ...
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bioRxiv - Physiology 2024Quote: ... Exendin-4-BSA-AF647 (Ex-4-BSA-AF647) was obtained by conjugation of Ex-4-BSA with Alexa Fluor 647 NHS ester (#A20006, Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: Intracellular calcium imaging was carried out on isolated taste bud cells loaded with 4 µM of the ratiometric calcium indicator Fura-2-acetoxymethyl ester (Fura-2 AM, Invitrogen) in Tyrode’s with 0.05% pluronic acid F-127 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+CD25+ Tregs from Apoe−/− or Ccr4−/−Apoe−/− mice were cocultured with carboxyfluorescein diacetate succinimidyl ester (Thermo Fisher Scientific)-labeled CD4+CD25− conventional T cells (2.5×104 cells ...
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bioRxiv - Bioengineering 2021Quote: ... a medium-chain fatty acid (Dodecanoic Acid, C12; BODIPY™ 500/510 C1, C12; D3823, Thermo Fisher Scientific Inc.) or a long-chain fatty acid (Hexadecanoic Acid ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Gibco; Thermo Fisher, Waltham ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for essential amino acid (EAA) mix and 1X MEM Non-Essential Amino Acids Solution (Gibco, Cat. #11140050) was used for non-essential amino acid (NEAA ...
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bioRxiv - Molecular Biology 2022Quote: ... C18 disk were washed 10 times with LC-MS grade water containing 0.1 % formic acid and subsequently eluted with 80% acetonitrile/0.1% formic acid and dried using a Speedvac (Thermo Scientific). Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... A 300 μL solution of 5% formic acid and 0.2% trifluoroacetic acid (TFA) R2 50 μm Poros (Applied Biosystems) beads slurry in water was added to the gel pieces before returning the samples to the shaker for an additional three hours at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... formic acid and trifluoroacetic acid (TFA) were liquid chromatography-mass spectrometry (LC-MS) grade solvents (Thermo Fisher Scientific, US). Ethanol was analytical grade (Chem-Supply ...
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bioRxiv - Neuroscience 2024Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Thermo Fisher] ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... amino acids 23-429) or pro- BMP10 (NP_055297.1, amino acids 22-424) was cloned into pcDNA3.1+ (Invitrogen/Thermo Fisher Scientific) downstream of the rat serum albumin signal peptide ...
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bioRxiv - Microbiology 2022Quote: Nucleic acid extraction was carried out using the MagMAX CORE Nucleic Acid Purification Kit (Applied Biosystems, Thermo Fisher Scientific). A pre-treatment step using bead beating tubes from MagMAX CORE Mechanical Lysis Module was introduced to improve DNA recovery ...
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bioRxiv - Microbiology 2022Quote: Nucleic acid extraction was carried out using the MagMAX CORE Nucleic Acid Purification Kit (Applied Biosystems, Thermo Fisher Scientific). A pre-treatment step using bead beating tubes from MagMAX CORE Mechanical Lysis Module was introduced to improve DNA recovery ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% acetic acid) and solvent B (80% acetonitrile, 0.5% acetic acid) into an Orbitrap Eclipse mass spectrometer (Thermo Scientific). High resolution full MS spectra were acquired with a resolution of 120,000 ...
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bioRxiv - Microbiology 2024Quote: ... Nucleic acid was extracted from 200 µl of supernatants using the MagMAX CORE Nucleic Acid Purification Kit (Applied Biosystems) on an automatic extractor KingFisher Flex (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... either Alexa Fluor568- or Alexa Fluor647-conjugated antibody in 3 % BSA/PBS (Invitrogen, 1:500). Following three PBS washes ...
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bioRxiv - Cell Biology 2021Quote: ... AsPC-1 and BxPC-3 cells were maintained in Roswell Park Memorial Institute (RPMI, Invitrogen) supplemented with 10% FBS and 4 mM L-glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... PerCP-eFluor 710 mouse anti-human CD223 (LAG-3) (1:100; 46-2239-41, Invitrogen), and BV421 mouse anti-human CD366 (TIM-3 ...
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bioRxiv - Cell Biology 2021Quote: ... DNA in adult testes was stained with To-Pro-3 (1:1000, Life Technologies, T3605) solution in the dark for 15min ...
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bioRxiv - Biochemistry 2022Quote: Cells were either transfected with PEI (1 μg/3 μg DNA) or lipofectamine 2000 (ThermoFisher) diluted in OptiMEM (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated for 3 min with Hoechst 33342 (Thermo Fisher Scientific, dilution 1/10,000) for live-cell nuclear fluorescence labeling.
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bioRxiv - Genetics 2020Quote: ... 3-Phosphoglycerate kinase (Pgk1) protein was detected with anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) and HSP90 protein was detected with a mouse anti-HSP90 monoclonal antibody (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with a 3:1 mixture of Newborn Calf Serum (NBCS) (Gibco, Thermo Fisher Scientific) and Fetal Calf Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with a 3:1 mixture of Newborn Calf Serum (NBCS) (Gibco, Thermo Fisher Scientific) and Fetal Calf Serum (FBS ...
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bioRxiv - Pathology 2020Quote: ... It consisted of the DMEM medium and F12 in ratio 1:3 (Gibco Life Technologies), supplemented with 2.5 ml FBS ...
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bioRxiv - Pathology 2020Quote: ... It consisted of the DMEM medium and F12 in ratio 1:3 (Gibco Life Technologies), supplemented with 2.5 ml FBS ...
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bioRxiv - Genetics 2020Quote: ... 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Zoology 2021Quote: ... and 3) an Alexa Fluor® 488-conjugated streptavidin (S11223, Thermo Fisher Scientific; 1:500) for 2 h at 28°C ...
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bioRxiv - Developmental Biology 2021Quote: ... the blastomeres were stained with 1 µM TO-PRO-3 iodide (Thermo Fisher Scientific, T3605) for 30 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605) at 1:2000 was performed for 2 hours before 3x 15 minute PBS-T washes and a final PBS-T wash overnight at 4°C while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were washed 3 times in PBS then incubated in 1:40 phalloidin (Thermo Fisher) in PBS for 20 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... 10 nl of 1 mM FluoZin-3 (FZ3, 847 Da, Thermo Fisher Scientific, Cat. F24194), 100 mM CaCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were rinsed 3 times with 1 mL of 1X PBS (Gibco, 10010-023), quenched in 1 mL of 0.1% sodium borohydride/PBS solution (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... in a ratio of 3:1 (FuGENE:DNA) and 65 μl of Opti-MEM (Thermo Fisher) was added ...