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Citations for Thermo Fisher :
3151 - 3200 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Differentiation of H9 ES cells was induced by exchanging human ES cell growth medium with DMEM/F12 (Gibco) containing 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: Human proteins (LIS1-SNAP, or motor domains) were expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications3,7,20,69 ...
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bioRxiv - Microbiology 2022Quote: Toxoplasma gondii tachyzoites were amplified in human foreskin fibroblasts (HFFs, ATCC) in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 5% of Fetal Bovine Serum (FCS ...
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bioRxiv - Physiology 2022Quote: Total RNA from human liver biopsies (10-20 mg) was extracted using Trizol extraction (Life Technologies, Carlsbad, CA) followed by a purification step using the GeneJET RNA Purification kit (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: Human bladder carcinoma 5637 cells (ATCC, HTB-9) were grown in RPMI-1640 media (GIBCO, cat.no. 21875-034) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney cells (HEK293T, ATCC, Cat#: CRL-3216) cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Fresh-frozen human postmortem brain tissues were sectioned at 10 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 10 minutes at -20°C and then vacuum sealed in plastic slide boxes and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (1132D) were purchased from Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: Human induced pluripotent stem cells (iPS line WTSIi008-A, EBiSC, UK) were cultured in E8 medium (Life technologies) on Geltrex coating (Life technologies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and human normal fibroblast cell line HS68 (ATCC #: CRL-1635) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... total RNA were extracted then reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher Scientific). The cDNA libraries were amplified and barcoded using Ion AmpliSeq Transcriptome Human Gene Expression core panel and Ion Xpress Barcode Adapter (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... at 4% haematocrit in supplemented RPMI media (RPMI-HEPES, 0.2% NaHC03, 5% heat-inactivated human serum [Australian Red Cross], 0.25% AlbumaxII [GIBCO] ...
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bioRxiv - Genomics 2019Quote: ... Primary antibodies (anti-human CD47-APC; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3; and clone B6H12.2, ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human MSCs at passage 2 were maintained in growth media consisted of alpha-MEM (Life Technologies, 32561--037) supplemented with 17% FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: DF-1 chicken fibroblasts and human HEK 293 or 293T cells were grown in Dulbecco’s modified Eagle’s Medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Biologos) ...
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bioRxiv - Developmental Biology 2019Quote: Human umbilical vein endothelial cells (HUVECs) were purchased from Yale Vascular Biology Center and cultured in M199 (Gibco) supplemented with 20% FBS (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: Human monocyte THP-1 cells from ATTC were maintained in Roswell Park Memorial Institute (RPMI-1640) medium (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2019Quote: ... NIH 3T3 and human epithelial (HepG2) cells were obtained from ATCC and grown in RPMI media (Life Technologies).
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bioRxiv - Pathology 2020Quote: ... Anti-human Alexa Fluor 594-antibody was used for detection (Molecular probes®, ThermoFisher Scientific, Carlsbad, CA, USA). For nuclear staining ...
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bioRxiv - Microbiology 2021Quote: Primary HFFF immortalised with human telomerase (HFFF-TERTs) (96) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Immunology 2020Quote: ... Raw data were searched with the Mascot search engine (MatrixScience, Boston, MA) against a recent human database (2015) on Proteome Discover Version 2.1.1.21 (Thermo Scientific) using the following parameters ...
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bioRxiv - Microbiology 2021Quote: ... Huh7 human hepatoma cells and Calu3 lung adenocarcinoma cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; from Gibco) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... media was fully exchanged (4 mLs) to endothelial cell medium (ECM): human Endothelial Serum-Free Medium (Invitrogen 11111) with 1% platelet-poor plasma-derived bovine serum (Biomedical Technologies 50-443-029 ...
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bioRxiv - Immunology 2020Quote: ... cells were stimulated for 24 h with Gibco™ Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher, 11131D) in a bead-to-cell ratio of 0.5:1 in simultaneous presence or absence of 2 mM 2-Fluoro-2-deoxy-D-glucose (2-FDG ...
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bioRxiv - Neuroscience 2019Quote: ... human Ad5 genomic plasmid (pBHGfrtΔE1,3FLP; Microbix) and each pDC511-LRRK2 shuttle plasmid were introduced by calcium phosphate (Invitrogen) transfection into stable Tet-On-inducible E2a cells (E2T cells ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Cancer Biology 2021Quote: Human adenoid primary B cells were stained with CellTrace Violet according to the manufacturer’s instructions (Thermo Fisher Scientific). Proliferation of CD19+ B cells was monitored by flow cytometry using BD Fortessa and the data were analyzed using the FlowJo software (Version 10.5.3).
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bioRxiv - Developmental Biology 2021Quote: ... hEPSCs were grown using ‘human Expanded Potential’ (hEP) medium consisting of DMEM/F12 (Thermo Fisher Scientific, 11320-033), Neurobasal-A (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Tetramers were mixed with the following anti-human antibody cocktail: anti-CD16 APC-eFluor780 (Invitrogen, #47-0168-41), anti-CD8α APC-eFluor780 (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... parasites were cultured in human red blood cells (RBC) in RPMI-1640 culture medium (Thermo Fisher # 53400-025) supplemented with 10% v/v Albumax I (Thermo Fisher # 11020039) ...
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bioRxiv - Cancer Biology 2020Quote: ... and human embryonic kidney epithelial cell line (HEK293T) were maintained at 37°C in RPMI 1640 (Life Technologies) and DMEM supplemented with 10% fetal bovine serum and Penicillin and Streptomycin (1mM) ...
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bioRxiv - Molecular Biology 2020Quote: SNP 6.0 analysis was performed by Aros AB (Denmark) using a Genome-Wide Human SNP Array 6.0 (Affymetrix) on RPE1 cells and two edited RPE1 lines derived from the parental RPE1 line ...
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bioRxiv - Evolutionary Biology 2020Quote: Cells in 6-well plates were transfected with 1 ug human or cow CD44-promoter-pNL2.1 or empty vector pNL2.1 using Lipofectamine 3000 (Invitrogen) as manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was extracted from both medial and adventitia layer of the aortic wall and subsequently sequenced on HTA 2.0 (Human Transcriptome Array 2.0 - Affymetrix) platform.
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bioRxiv - Neuroscience 2021Quote: ... 200 µg of human serum) were separated by SDS-PAGE on 3-8% tris-acetate gels (NuPAGE, Invitrogen) under reducing conditions at 150 V (cellular fraction ...
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bioRxiv - Cancer Biology 2021Quote: Cells were stimulated for a total of 6 hours with human T-activator anti-CD3/CD28 Dynabeads (ThermoFisher) stimulation with GolgiStop solution being added for a total of 5 hours block intra-cellular protein transport (BD Bioscience) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Synthetic Biology 2021Quote: BMP4 concentration released into the media was quantified using the BMP-4 Human ELISA Kit from (ThermoFisher, EHBMP4). Standards and protocols were done according to the instructions of the kit ...
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bioRxiv - Immunology 2021Quote: ... and anti-Spike IgG was quantified using a Human SARS-CoV-2 Spike (Trimer) IgG ELISA Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Human retinal pigment epithelial ARPE-19 cells (ATCC® CRL-2302™) were cultivated in DMEM/F12 (Gibco) with 10% fetal bovine serum (Lonza) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Addgene No. 154106) are previously described. Human codon-optimized S (GenBank No. YP_009724390.1) was subcloned into pCEP4 (Invitrogen) from pUC57-2019-nCoV-S(Human ...
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bioRxiv - Cell Biology 2020Quote: ... anti-human Ig Alexa Fluor 488 (A11013) or anti-rabbit Ig Alexa Fluor 568 (A11036; Thermo Fisher Scientific), according to primary antibody species.
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bioRxiv - Cell Biology 2020Quote: The human bronchial epithelial cell (HBEC6-KT) line was cultured in Keratinocyte Serum-Free Medium 1X (Gibco®) according to the manufacturers directions and supplemented with P/S (VWR® ...
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bioRxiv - Cancer Biology 2021Quote: ... Monoclonal human osteosarcoma cell (U2OS 3XFlag-HaloTag-Atg9A, see below) were cultured in RPMI media (Gibco/ThermoFisher, A4192301) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... Monoclonal human osteosarcoma cell (U2OS 3XFlag-HaloTag-Atg9A, see below) were cultured in RPMI media (Gibco/ThermoFisher, A4192301) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with FACS buffer and stained with goat anti-Human IgG Alexa Fluor 647 (Invitrogen) or goat anti-Mouse IgG Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in DMEM+GlutaMAX-I (Thermo Fisher Scientific) supplemented with heat-inactivated 8% FBS.
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed using buffer sets of Human Regulatory T-cell Staining Kit (eBioscience/Thermo Fisher Scientific), following the manufacturer’s protocol ...