Labshake search
Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... The sections were counterstained with the nucleic acid stain Hoechst 33342 (1 μg/ml; Invitrogen) in PBS ...
-
bioRxiv - Developmental Biology 2024Quote: ... Fixed embryos were stained with 1 µL Sytox green (Invitrogen, Sytox Green Nucleic acid, S7020) in 1 mL PBST and incubated at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 1 x MEM Non-Essential Amino Acids Solution (Gibco; Cat. No. 11140-050), 1 x N2 supplement (Gibco ...
-
bioRxiv - Microbiology 2024Quote: ... Nucleic acids were extracted with equal volume of ultrapure phenol:chloroform:isoamylic alcohol (25:24:1) (ThermoFisher) followed by extraction with chloroform alone ...
-
bioRxiv - Microbiology 2019Quote: ... Rabbit anti-sonnei (1:100) (phase 1 and 2 sera, Fisher Scientific) or rabbit anti-flexneri (1:500 ...
-
bioRxiv - Genomics 2019Quote: ... ERCC spike-in control (Thermo Fisher, mix 1, dilution 1:2 millions) was added to each well (except for the negative control) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2 x 106 cells mixed 1:1 with Matrigel (Fisher Scientific, 356237) and were subcutaneously implanted into both flanks of the mice ...
-
bioRxiv - Immunology 2022Quote: ... Whole blood samples were diluted 1:1 with PBS+2%FBS (Gibco) and subjected to Ficoll (Cytiva ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:1000 2-Mercaptoethanol (ThermoScientific, 21985023) and 1:1000 Gentamicin (Gibco, 1570060). After collection ...
-
bioRxiv - Bioengineering 2021Quote: ... Lauric acid (dodecanoic acid, LA, 99%, Figure S1(c)) was obtained from Acros Organics. The phospholipids DOPC (1,2-dioleyoyl-sn-glycero-3-phosphocholine ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Formic acid (FA) and hydrochloric acid were purchased from Fisher Scientific (Portsmouth, NH, USA). Stock solutions were dissolved in water individually at a final concentration of 1 mg/mL ...
-
bioRxiv - Genomics 2021Quote: The metabolism of 4-hydroxyphenylacetic acid (p-hydroxyphenylacetic acid; Acros Organics, Product code: 121710250) and gentisic acid (2,5-dihydroxybenzoic acid ...
-
bioRxiv - Microbiology 2024Quote: ... Nucleic acid extraction was performed with the MagMAX CORE Nucleic Acid Purification Kit (ThermoFisher)81 ...
-
bioRxiv - Bioengineering 2021Quote: ... a 6 well plate containing 6 mL FACS Clean (Thermo Fisher, BD 340345), 6 mL FACS Rinse (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... and labelled with 0.7 μM 6-carboxyfluorescin dictate (6-CFDA; Thermo Fisher Scientific) for 30 minutes at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... high dose IL-6 stimulation received 100 ng/ml IL-6 (Gibco; PHC0066) or 100 pM acetic acid vehicle stimulation for either 3-or 24-hours and immediately collected for analysis ...
-
bioRxiv - Neuroscience 2022Quote: ... an internal solution containing 1 mM Fura-2 (K5-Fura-2, cell impermeable, Invitrogen F1200), 140 mM KCl ...
-
bioRxiv - Microbiology 2019Quote: Spotted protein arrays (5 x 6) were printed onto each well of Greiner high-binding 96-well plates (Cat# 655097, Thermo Fisher Scientific) using a sciFLeXARRAYER S12 (Scienion AG ...
-
bioRxiv - Molecular Biology 2021Quote: For DNA plasmids: 5×106 HEK293T cells were grown in 6-well plates and Lipofectamine™ 3000 Transfection Reagent (L3000001, ThermoFisher UK) was used to transfect the cells following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: Exon 2 of A3A and exons 5 and 6 of A3B with either 20 or 80 bp of flanking intronic sequences were synthesized (Thermo Fisher Scientific) and cloned in sense orientation using XhoI and NotI restriction sites of Exontrap vector pET01 (MoBiTec ...
-
bioRxiv - Microbiology 2019Quote: ... isolated for each sediment-depth explored along SSK42/5 and 6 were shotgun sequenced individually on an Ion Proton sequencing platform (Thermo Fisher Scientific) using 200 nucleotide read-chemistry ...
-
bioRxiv - Biophysics 2019Quote: ... the final dye to protein ratio was typically 5-6 dye molecules per protein molecule as measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). Cells in suspension culture dishes were labeled with 0.5 nM fluorescent IgE for typically 12-20 hours before imaging.
-
bioRxiv - Molecular Biology 2019Quote: ... which consisted of 2.6 μl NFW, 5 μl TATAA SYBR Green mix (TATAA, Sweden) and 0.4 μl of 10 μM primers (Thermo Fisher Scientific, USA). Cycling protocol consisted of initial enzyme activation at 95°C (t = 3 min) ...
-
bioRxiv - Biochemistry 2021Quote: ... The mixture was left to equilibrate for 5 min at RT before 6 μL aliquots were placed in crystal-clear vials (Fisher Scientific UK) and snap-frozen in liquid nitrogen ...
-
bioRxiv - Microbiology 2021Quote: ... was introduced into 5 × 106 C6/36 mosquito cells per well of a 6-well plate via Lipofectamine 2000 reagent (Thermo Fisher Scientific), following the product’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The specificity of this antibody has been previously validated by us and others by Western Blot, IF and IHC analyses (5, 6).The Anti-NeuN mouse monoclonal antibody (ThermoFisher MA5-33103) was used for IF ...
-
bioRxiv - Zoology 2022Quote: ... or 5,6,11-trideoxyTTX (10−5 M) together with a dextran conjugated with Alexa Fluor 555 (M.W. 10,000, 10−6 M; D34679, Thermo Fisher Scientific, MA, USA) for 5 min ...
-
bioRxiv - Immunology 2020Quote: ... or aged (26-28 mo) female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco, 11995-073) supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2021Quote: ... 5 x 105 HCT116 cells were seeded in a 6 well plate with Dulbecco’s modified Eagle medium (DMEM, Gibco®, Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... The syn-gRNA oligos and syn-gRNA-NTC were transfected using Lipofectamine RNAiMAX transfection reagent (5 µl per 6-well; Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... C57BL/6 P4-5 mice pups were rapidly decapitated and brains were processed in Hibernate-A medium (Thermo Fisher Scientific, A12475-01). Resulting tissue was enzymatically disassociated in buffer containing HEPES-HBSS with DNase (Worthington Biochemical LS002007 ...
-
bioRxiv - Developmental Biology 2022Quote: ... NC cells were aggregated into 3D spheroids (5 million cells/well) in Ultra Low Attachment 6-well culture plates (Fisher Scientific, 3471) and cultured in Neurobasal (NB ...
-
bioRxiv - Cell Biology 2023Quote: ... coated 6-well plates at 5×105 cells/well in RPMI 1640 with B27 and 10% knockout serum replacement (Thermo Fisher Scientific). Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies) ...
-
bioRxiv - Biophysics 2022Quote: ... EVs were stained with 5-(and-6)-Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE, hereinafter referred as CFSE) (Thermo Fisher, Catalog No. C1157) and separated as described previously [10] ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were seeded in 6-well plates and immediately transfected with a mixture of 10 μL Lipofectamine2000 (Thermo Fisher Scientific), 6 μL of 20 μM target-specific siRNA (or non-targeting siRNA as control) ...
-
bioRxiv - Immunology 2021Quote: ... 1 mM sodium pyruvate solution and 1% MEM non-essential amino acids solution(100X) (Gibco by Life Technologies, NY, USA). Murine monoclonal IgG1 anti-human CD13 (Mab C ...
-
bioRxiv - Immunology 2021Quote: ... 1 mM sodium pyruvate solution and 1% MEM non-essential amino acids solution(100X) (Gibco by Life Technologies, NY, USA). Murine monoclonal IgG1 anti-human CD13 (Mab C ...
-
bioRxiv - Bioengineering 2022Quote: ... To visualize ssDNA patches during time lapse imaging (figure 1) we stained ssDNA with 1-5x SYBR Gold nucleic acid gel stain (S11494, ThermoFisher) in 1x DPBS for 5-10 minutes and washed twice in 1x DPBS before introducing culture medium ...
-
bioRxiv - Biochemistry 2023Quote: ... cultured in Minimum Essential Medium (MEM) supplemented with 1 % sodium pyruvate and 1 % non-essential amino acids (Gibco, Invitrogen®). All media were supplemented with GlutaMAX® (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: ... to KSR base medium (Knockout-DMEM, 15% knockout-serum replacement, 1% Glutamax, 1% nonessential amino acids, 100 mM b-mercaptoethanol, (ThermoFisher)) ...
-
bioRxiv - Biochemistry 2023Quote: ... cultured in Minimum Essential Medium (MEM) supplemented with 1 % sodium pyruvate and 1 % non-essential amino acids (Gibco, Invitrogen®). All media were supplemented with GlutaMAX® (Gibco ...
-
bioRxiv - Bioengineering 2023Quote: ... was reduced on the 78 ± 3 nm cores using 1 ml of 1 mM L-ascorbic acid (AA) (Fisher Scientific), making core@shell structures ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 and HeLa cells were maintained in CA media (DMEM + 1% non-essential amino acids + 1% L-Glutamine + 10% FBS heat inactivated + 1.43 mM-β-mercaptoethanol) on 100 mm (Nunc) dishes ...
-
bioRxiv - Bioengineering 2020Quote: ... Bone marrow human macrophage (KG-1 ATCC® CCL246™) passages 2–5 were cultured in cell suspension flasks using IMDM medium (Gibco™ GlutaMAX™, HEPES) with 20% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... each well was washed twice in ice-cold PBS and the cells were scraped off in ice-cold PBS with added protease inhibitors (1:100 Halt, cat nb. 78429, Thermofisher; 5 mM NAM; 2 mM NaOV). The suspension was transferred to a tube and centrifuged for 10 min at 2000g at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... before they were transferred into 2° antibodies dilute (1:700 of Dylight-/Alexa-conjugated antibodies made in donkey purchased from Thermo Fisher Scientific in 5% BSA/PBST) for 2 hrs at RT ...
-
bioRxiv - Immunology 2022Quote: ... Harvested naïve CD4+ T cells were plated at a density of 1.5 × 105 cells/mL in 2 ml of complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies], and 0.05% (50μM) 2-ME [Sigma-Aldrich]) ...
-
bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...
-
bioRxiv - Neuroscience 2019Quote: ... The resulting suspension with all nuclei was stained with the fluorescent DNA intercalant marker DAPI (4’-6-diamidino-2-phenylindole dihydrochloride, Invitrogen, USA) diluted 1:20 from a stock solution of 20 mg/L and filled with PBS 0.1M to known volume ...
-
bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...