Labshake search
Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Then 2-5 µg of RNA were reverse transcribed using the Superscript III reverse transcriptase (Invitrogen) and oligo dT primer (Invitrogen) ...
-
bioRxiv - Molecular Biology 2024Quote: Unfragmented UHRR or HEK-293T RNAs (5 μg) were DNased with 2 U TURBO DNase (Invitrogen) and purified using an RNA Clean & Concentrator kit (Zymo Research ...
-
bioRxiv - Cancer Biology 2024Quote: A 5-ethynyl-2′-deoxyuridine (EdU) incorporation assay was performed according to the manufacturer’s protocol (Invitrogen). Hydrogel-embedded PCLS were incubated for 16 hours in complete culture media with 10 μM EdU ...
-
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... After 16 h incubation at 37°C in a 5% CO2 chamber (Cytoperm 2, ThermoFisher Scientific), the culture supernatants were collected and stored at –20°C until further analysis.
-
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... After 24 h incubation at 37°C in a 5% CO2 chamber (Cytoperm 2, ThermoFisher Scientific), the culture supernatants were collected and stored at –20°C until further analysis.
-
bioRxiv - Genetics 2024Quote: ... Fungal nuclei were stained 5 min before imaging with 2 µg/ml Hoechst 33342 (Invitrogen™) in water in the dark ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 µg of peptides in 5 % (v/v) formic acid (approximately 10 µL) was injected onto an Acclaim™ PepMap™ C18 nano-trap column (Thermo Scientific). After washing with 2 % (v/v ...
-
bioRxiv - Microbiology 2020Quote: ... aliquots were diluted 1:2 and 1:5 in 2M urea solvent and their total protein concentration was measured using a Pierce Bicinchoninic Acid Protein Assay (Thermo Scientific, Waltham, MA) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
-
Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: Cortical neurons at DIV10-14 were treated for 24 hours with 10 mM glutamine or 5 mM aspartic acid in Neurobasal-A medium (Invitrogen, Cat# A24775-01) supplemented with 1 mM pyruvate and 1 mM lactate ...
-
bioRxiv - Molecular Biology 2023Quote: ... Dried TMT-labelled peptides were reconstituted in 5 uL of 0.1% formic acid and on-line eluted into a Fusion Tribrid mass spectrometer (Thermo Scientific, San Jose, CA) from an EASY PepMapTM RSLC C18 column (2μm ...
-
bioRxiv - Neuroscience 2021Quote: ... Sodium pyruvate (2 mM), MEM amino acids solution (50X, without L-Glutamine) and MEM vitamins solution (100X) were added from concentrates (Fisher Scientific, UK). The pH was adjusted to 7.5 (osmolality ~308 mmol kg-1).
-
bioRxiv - Neuroscience 2021Quote: ... Neural precursors were manually picked and transferred to 25 cm2 flasks in NIM medium supplemented with B27 and ascorbic acid (NIM + 2% B27 supplement (Gibco, 17504-044), 0.1% ascorbic acid (Sigma Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... the rats were injected with ketamine (50 mg/kg; i.p.) before intra-rectal administration of 2 ml acetic acid (AA) (Fisher Scientific, Massachusetts, USA), 4% (v/v ...
-
bioRxiv - Microbiology 2022Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The peptides were then vacuum dried and resuspended in 20 μl of 2% ACN and 0.1% formic acid for loading onto the Q-Exactive™ HF Mass Spectrometer (Thermo Scientific™).
-
bioRxiv - Microbiology 2022Quote: ... Roughly 1/3 of the plate (2-3 loopfuls) were added to 400 µl 1x Tris-Ethylenediaminetetraacetic acid (EDTA) pH 7.5 (Thermo Scientific, Waltham, USA) and heated at 80 ºC for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: A dual system was developed to trace both 0,57 mg/mL FITC-Dextran and 2 μM of the long-chain fatty acid analogue BODIPY-C12 (558/568) (ThermoFisher, cat. #D3835) in one well ...
-
bioRxiv - Systems Biology 2023Quote: Samples were resuspended in 4% formic acid/2% acetonitrile solution and analyzed on an Q-Exactive Plus MS system (Thermo Fisher Scientific) equipped with an Easy nLC 1200 ultra-high pressure liquid chromatography system (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... Extraction of SARS-CoV-2 RNA was performed using the MagMax Viral/Pathogen Nucleic Acid Isolation kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
-
bioRxiv - Biochemistry 2023Quote: The peptides were resuspended in 0.1% formic acid in water and 2 μg loaded onto a UltiMate 3000 RSLCnano System attached to an Orbitrap Exploris 480 (Thermo Fisher Scientific). Peptides were injected on an Acclaim Pepmap trap column (Thermo Fisher Scientific #164564-CMD ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were concentrated onto the trap column at 5 μL/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap 480™ mass spectrometer (Thermo Fisher Scientific) equipped with a FAIMS Pro interface at 300 nL/minute ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) at 150V for 2h using NuPAGE 2-(N-morpholino)ethanesulfonic acid SDS running buffer (NP0002, Thermo Fisher Scientific), NuPAGE Antioxidant (NP0005 ...
-
bioRxiv - Immunology 2023Quote: ... After 18 h cells were mock transfected or transfected with 2-5A (20 μM) and stained for total cells with SytoTM 60 red fluorescent nucleic acid stain (250 nM) (ThermoFisher Scientific, #S11342) (converted to blue by IncuCyte software ...
-
bioRxiv - Microbiology 2023Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
-
bioRxiv - Cell Biology 2023Quote: ... Confirmation of PCR reaction was carried out on a 1% w/v agarose in 1x TBE (89 mM tris, 89 mM boric acid, 2 mM EDTA) gel containing 1x SYBR™ Safe DNA gel stain (Invitrogen™) using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs) ...
-
bioRxiv - Bioengineering 2019Quote: ... denaturing gels were run with 6 μL per well of a 1:1 mixture of sample and Gel Loading Buffer II with formamide (Ambion) in a 10% TBE-Urea ...
-
bioRxiv - Physiology 2021Quote: ... we incubated the slices for 50 minutes at RT in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03% Pluronic F-127 (w/v) ...
-
bioRxiv - Physiology 2022Quote: ... The prepared slices were stained in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03 % Pluronic F-127 (w/v) ...
-
bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
-
bioRxiv - Neuroscience 2022Quote: ... embryoid bodies were embedded in 15 µL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation media consisting of 1:1 DMEM-F12 and Neurobasal media (Gibco), with addition of 0.5% N2 supplement (Life Technologies) ...
-
bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged once a week (∼80% confluence/well) at 1:4-1:6 ratios using 1mg/ml collagenase (Gibco) to detach colonies and onto fresh ir-MEF plates.
-
bioRxiv - Cancer Biology 2023Quote: ... per 1×104 SiHa and 1×103 HeLa cells/6-well culture dish were transfected with Lipofectamine RNAi-Max (Invitrogen). Efficient knock down was confirmed by western blot analysis ...
-
bioRxiv - Cell Biology 2023Quote: ... 1.2 M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 µg/mL DAPI (4’, 6-diamidino2-phenylindole; Molecular Probes). Cells were imaged with a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
-
bioRxiv - Bioengineering 2020Quote: ... CD8+ T cells were cultured in a 1:1 mix of Aim 5 (Gibco) and RPMI ...
-
bioRxiv - Cell Biology 2022Quote: ... and Renilla luciferase at a 1:30:1:5 ratio using Lipofectin (Thermo Fisher) according to manufacturer’s protocol ...
-
bioRxiv - Physiology 2020Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 15% FBS (Atlas Biologicals) ...
-
bioRxiv - Physiology 2020Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 15% FBS (Atlas Biologicals) ...
-
bioRxiv - Immunology 2020Quote: ... 1×106 cells were incubated with 5□μg/mL of Indo□1 AM (Invitrogen) and 0.5□μg/mL of pluronic F□127 (Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... media was changed to 1:1 KSR:N2B media with puromycin (5 μg/ml, Gibco). On day 3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 10% FBS (Cytiva HyClone) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 10% FBS (Cytiva HyClone) ...
-
bioRxiv - Cell Biology 2020Quote: ... 2% BSA and 2 mM EDTA at 1 x 106 cells per 100 μl: anti-ITGA7 APC 1:500 (Invitrogen MA5-23555) and anti-SCA-1 FITC 1:50 (BD Pharmingen 557405) ...
-
bioRxiv - Genetics 2020Quote: ... A single gRNA and single strand HDR oligo were co-transfected along with the gRNA (1-2 μg of each plasmid) into 1-2 × 106 WT ESCs using Lipofectamine 2000 (Invitrogen #1168-019) in a single well of a 6 well plate ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
-
bioRxiv - Bioengineering 2024Quote: ... and HITI insert were first mixed with a ratio of 2:2:1 with the reagent P3000 (ratio of DNA: P3000 of 1:2) in Opti-MEM™ Reduced Serum Medium (Gibco™), and then added to Opti-MEM™ Reduced Serum Medium (Gibco™ ...
-
bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The following fatty acid azides were used: C15-az (15-azidopentadecanoic acid) from Life Technologies and C17-az (17-azidoheptadecanoic acid ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Uric acid concentration was measured using the Amplex Red Uric Acid/Uricase kit (ThermoFisher A22181) following the manufacturer instructions ...