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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The resuspended peptides in sample loading buffer (2% acetonitrile and 0.05% trifluoroacetic acid) were separated and analysed on an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific) coupled online to either a Q Exactive HF or an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Then the pellets were resuspended in 10 mL of sterile water and transferred to a 15 mL falcon tube containing 2 g of acid washed glass beads (0.5 mm of diameter, Thermofisher, G8772-100G). Cells were then disrupted using a Fastprep system (MP Biomedicals) ...
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bioRxiv - Biophysics 2022Quote: ... Electron microscopy grids were prepared in 2% (w/v) phosphotungstic acid (pH 7.5) and were examined in a Talos L120C transmission electron microscope (FEI-Thermo Fisher Scientific). Nanodisc samples were frozen in aliquots and stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptide extracts obtained were dissolved in 17 μl of 0.05% trifluoroacetic acid in 2% acetonitrile and analyzed by online nanoLC using an Ultimate 3000 RSLCnano LC system (Thermo Scientific Dionex) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
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bioRxiv - Pathology 2024Quote: ... the peptide was reconstituted in 2% acetonitrile/0.1% formic acid (FA) and analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific) coupled online to an UltiMate 3000 RSLCnano System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Q-Exactive plus Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...
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bioRxiv - Microbiology 2023Quote: C18 purified digests were re-suspended in Buffer A* (2% acetonitrile, 0.01% trifluoroacetic acid) and separated on a Ultimate 3000 UPLC (Thermo Fisher Scientific) with a two-column chromatography set up composed of a PepMap100 C18 20 mm x 75 μm trap and a PepMap C18 500 mm x 75 μm analytical column (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Eclipse Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface both at 300 nl/minute via the analytical columns using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... iRBCs were washed twice with media to remove extracellular merozoites before being aliquoted in triplicate into a 96-well U-bottomed plate and stained with the nucleic acid dye SYTO-61 (2 μM) (Thermo Fisher) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: Proteome samples were re-suspended in Buffer A* (0.1% trifluoracetic acid, 2% acetonitrile) and separated using an Ultimate 3000 UPLC (Thermo Fisher Scientific) equipped with a two-column chromatography set up composed of a PepMap100 C18 20 mm x 75 μm trap and PepMap C18 500 mm x 75 μm analytical column (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: Dried peptides were suspended in 0.1% formic acid (FA) with 2% ACN and analyzed by LC-MS/MS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) connected to an EASY nLC 1200 (buffer A ...
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bioRxiv - Biophysics 2024Quote: ... 2 μL of the total primary stock was pipetted into 500 μL neat (70%) nitric acid (Trace Metal Grade, Fisher Scientific) in a 10 × 75 mm borosilicate glass tube (Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Fractionated samples were reconstituted with 2% acetonitrile/0.1% formic acid and analyzed on an EASY-nLC 1200 system (Thermo Fisher Scientific) coupled to an Orbitrap EclipseTM TribridTM Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 mL HisTrap column was washed with 10 column volumes of wash buffer (2× GIBCO 14200-075 PBS, 5 mM Imidazole, pH 7.5) followed by 6 column volumes of elution buffer (2× GIBCO 14200-075 PBS ...
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bioRxiv - Biochemistry 2019Quote: ... 300 μm × 5 mm, 5 μm, 100 Å, separation column: C18, Acclaim PepMap, 75 μm × 500 mm, 2 μm, 100 Å, Thermo Fisher Scientific). After loading the sample on the precolumn ...
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bioRxiv - Biochemistry 2024Quote: ... 300 μm × 5 mm, 5 μm, 100 Å, separation column: C18, Acclaim PepMap, 75 μm × 500 mm, 2 μm, 100 Å, Thermo Fisher Scientific). After loading the sample on the pre-column ...
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bioRxiv - Biochemistry 2022Quote: ... a 1 ml click reaction containing 5 μl 1 mM Azide-488 (Invitrogen), 100 μl 20 mg/ml sodium ascorbate ...
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bioRxiv - Molecular Biology 2021Quote: ... claudin-5 (1:100) and occludin (1:50) (all from Thermo Fisher Scientific) at 4 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5-1 μg DNA or 1-5 μl Lipofectamine 2000 (11668-030, Invitrogen) was diluted in Opti-MEM at room temperature (22°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 ng of end-labeled pNG1175 DNA was mixed with 1 µL streptavidin-functionalized paramagnetic beads (M-270 Dynabeads, diluted 1:6 in 1X PBS with 0.1% BSA; Invitrogen) in 10 µL PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSC at day 6-7 after passaging were treated with a 1:1 mixture of TrypLE Select (Life Technologies) and 0.5 mM EDTA/phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... and an oxygen-scavenger system (60□mg ml−1 glucose oxidase, 6□mg□ml−1 β-ME (Gibco, 21985023)) were used for all STORM imaging experiments.
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bioRxiv - Cell Biology 2024Quote: ... a 6-well was coated with 1 mL of a 1:200 solution of either Geltrex (Gibco™ A1413202) or Cultrex (Bio-Techne 3445-005-01 ...
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bioRxiv - Cell Biology 2024Quote: ... coverslips with the gel attached were transferred to a well of a 6-well plate with 1 ml of digestion solution (0.5% Triton X100, 1 mM EDTA pH 8 (AM9260G, Ambion), 50 mM Tris pH 8 (AM9855G ...
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bioRxiv - Bioengineering 2022Quote: ... 100 U/mL of streptomycin) (HyClone),1% (v/v) MEM non-essential amino acid (Gibco). Human monocytic U937 cells (ATCC ...
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bioRxiv - Molecular Biology 2020Quote: ... for 0.5–1 hr at 37°C and re-purified using Acid-Phenol/Chloroform (Ambion). In some cases ...
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bioRxiv - Genetics 2020Quote: ... 0.15 μL of SYBR Green I Nucleic Acid Gel Stain diluted 1:100 (Thermo Fisher) and 5 μL of High-Fidelity 2X PCR Master Mix ...
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bioRxiv - Immunology 2020Quote: ... 1% minimum essential medium nonessential amino acids (100x MEM NEAA; Gibco Cat No 11140-035) and 1 mM sodium pyruvate (100 mM ...
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bioRxiv - Immunology 2020Quote: ... 1% 100X non-essential amino acids) plus 1μg/ml puromycin and 10μg/ml blasticidin (Invitrogen)) ...
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bioRxiv - Microbiology 2020Quote: ... 50 U.mL−1 penicillin/streptomycin and non-essential amino acids (all reagents from Invitrogen, UK). All cells were maintained in a 5 % CO2 atmosphere at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... approximately 50 ng of nucleic acid was mixed with either RNase A (Ambion; 1 μg), DNase I (Zymo Research ...
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bioRxiv - Immunology 2022Quote: ... before being quenched with 25 μL of 1N hydrochloric acid (Fisher Scientific, cat. # SA48-1). Plates were analyzed on a BioTek plate reader at 450 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... before being quenched with 25 μL of 1N hydrochloric acid (Fisher Scientific, cat. # SA48-1) and read on BioTek plate reader at 450 nm wavelength ...
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bioRxiv - Genetics 2022Quote: ... TO-PRO-3 Iodide carbocyanine monomer nucleic acid stain (1:1000, Thermo Fisher, cat# T3605) was used to stain DNA ...
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bioRxiv - Immunology 2022Quote: ... with 0.1% formic acid in water as solution A (LS118-1, Optima, Thermo Scientific, USA) and 0.1% formic acid in acetonitrile (LS120-1 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 1% MEM Non-essential amino acids solution (11140-050, ThermoFisher, Waltham, MA, USA) and 10% FBS (SH30396.03 ...
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bioRxiv - Molecular Biology 2022Quote: ... The sections were counterstained with the nucleic acid stain Hoechst 33342 (1 µg/ml; Invitrogen) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% (v/v) non-essential amino acids (catalog number 11140050, Gibco / Thermo Fisher Scientific). Differentiation into a co-culture of non-dividing cells that exhibit neuronal and astrocytic characteristics was initiated with the removal of growth factors and heparin salt from media ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% (v/v) non-essential amino acids (catalog number 11140050, Gibco / Thermo Fisher Scientific). Differentiation into a co-culture of non-dividing cells that exhibit neuronal and astrocytic characteristics was initiated with the removal of growth factors and heparin salt from media ...
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bioRxiv - Biophysics 2024Quote: ... 10% acetic acid microwaved for 1 minute and then stained with Colloidal Blue Stain (Invitrogen) overnight and de-stained in water and scanned the following day ...
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bioRxiv - Neuroscience 2024Quote: ... the tissues were counterstained with the nucleic acid stain Hoechst 33342 (1 μg/ml; Invitrogen) in PBS ...
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bioRxiv - Immunology 2024Quote: ... Caco2 cells culture medium was supplemented with 1% MEM Non-Essential Amino Acids (Gibco 11140035). All viral inoculations and infections were made in DMEM containing 2% FCS (D2).
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bioRxiv - Cancer Biology 2023Quote: ... and 1% minimum essential medium (MEM) Non-Essential Amino Acids 100x (NEAA, Gibco, #11140-035). For MDA-MB-231 with stable shRNA-mediated MCT4 KD (described in (Andersen et al. ...
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bioRxiv - Biochemistry 2023Quote: ... the cytosolic fraction was diluted with 1% nitric acid (trace metal grade, Thermo Fisher Scientific). The mitochondrial fraction was briefly digested with concentrated nitric acid (trace metal grade ...