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Citations for Thermo Fisher :
3101 - 3150 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... PCSK9 peptides were conjugated to Qß bacteriophage VLPs using succinimidyl 6-((ß-maleimidopropionamido)hexanoate) (SMPH; Thermo Fisher Scientific), as described previously60 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 50 cm (2 µm particle size) EASY-Spray column (Thermo Fisher Scientific, UK), which was assembled on an EASY-Spray source (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were fractionated using high-pH liquid-chromatography on a micro-flow HPLC (Dionex U3000 RSLC, Thermo Scientific). 45µg of pooled and purified TMT labelled peptides resuspended in Solvent A (5mM ammonium-bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific). Samples were loaded onto a trap column (Acclaim PepMap 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were separated on an in-house 50 cm column (360 μm od, 75 μm id, Fisher Scientific) packed with ReproSil-Pur C18 (120 Å pore size ...
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bioRxiv - Plant Biology 2023Quote: Peptides were analyzed with 5 different MS methods on a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific), summarized in Supplementary Dataset S7 ...
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bioRxiv - Developmental Biology 2023Quote: Raw mass spectrometry data was analyzed for peptide identification and quantification with Proteome Discoverer 2.5 (Thermo Fisher Scientific), using the SequestHT search engine against the Homo sapiens UniProt database (UP000005640 ...
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bioRxiv - Systems Biology 2023Quote: Tryptic peptides were separated by reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with an Acclaim C18 PepMap RSLC column using a 3-42% acetonitrile gradient over 60 minutes ...
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bioRxiv - Microbiology 2023Quote: Peptides were separated on a C18 nano-column (PepMap 100 C18 series. Thermo Fisher Scientific. Waltham, MA, USA) using an Ultimate 3000 nano-HPLC ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were trapped on a 2 cm nanoViper precolumn (i.d. 75 μm; C18, Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2:98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... peptide samples were desalted and subjected to analysis using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). For perturbation experiments (either coRIC or total proteome) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Aliquots of 30 µg peptides were labelled using TMTpro 18-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting tryptic peptides in 10 μL solution was loaded onto a 2-cm trap column (Thermo Scientific) and separated on a 50-cm EASY-Spray analytical column (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: Desalted SILAC-labeled peptides were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) equipped with a Thermo EASY-nLC 1200 LC system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were separated by reverse-phase chromatography on an 25-cm Easy Spray column (Thermo Fisher Scientific, USA) connected to an EASY-nLC 1200 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were loaded onto a trap column (PepMap 100 C18, 5 μm, 5 × 0.3 mm, Thermo Fisher Scientific) at a flow rate of 10 μL/min using 0.1% TFA as loading buffer ...
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bioRxiv - Microbiology 2023Quote: ... about 300 μg tryptic peptides were separated using a nanoflow DIONEX Ultimate 3000 RSLC nano System (ThermoFisher Scientific) with an XBridge Peptide BEH C18 column (300 Å ...
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bioRxiv - Microbiology 2023Quote: ... Peptide separation was carried out using an Acclaim PepMap trap column (75 μm × 2 cm; Thermo Fisher Scientific) and an EASY-Spray ES902 column (75 μm × 250 mm ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were trapped on a µPrecolumn C18 PepMap100 (5μm, 100 Å, 300 μm×5mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Cell Biology 2023Quote: Peptides were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a 2 cm nanoviper Precolumn (i.d. 75 μm, C18 Acclaim PepMapTM 100, Thermo Scientific) at a flow rate of 3.0 µL/min in buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were analyzed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) in data-dependent mode ...
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bioRxiv - Developmental Biology 2024Quote: ... On-line peptide separation was performed on a 15-cm EASY-Spray™ C18 column (ES75150PN, Thermo Fisher) by applying a linear gradient of increasing ACN concentration at a flowrate of 200 nL/min ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated using an Ultimate 3000 nano rapid separation liquid chromatography system (Dionex, ThermoFisher Scientific, MA, USA) equipped with a trapping cartridge (Precolumn C18 PepMap100 ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting peptides were extracted from gel fragments and analyzed using Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) combined with UltiMate 3000 RSLC nano-flow HPLC (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of the reconstituted peptides were separated on an EASY-nLC™ 1200 chromatography system (Thermo Scientific) coupled to a Q Exactive HF-X Orbitrap LC-MS/MS System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: The purified peptides were analyzed using the reverse phase liquid chromatography system Easy-nLC 1200 UHPLC (Thermo Scientific) coupled to an Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated on a 25-cm PepMap C18 column packed with 2-μm particles (Thermo Fisher Scientific) using the mobile buffer consisted of 0.1% FA in ultra-pure water with an eluting buffer of 0.1% FA in 100% ACN with a 60 min linear gradient of 5 to 25% ACN/0.1% FA at a flow rate of 300 nl/min.
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bioRxiv - Physiology 2024Quote: ... Peptides (360 ng) were loaded (Acclaim PepMap100 C18 3µm beads with 100 Å pore-size, Thermo Fisher Scientific) and separated (1.9-µm particle size C18 ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated on a 25-cm PepMap C18 column packed with 2-μm particles (Thermo Fisher Scientific) using the mobile buffer consisted of 0.1% FA in ultra-pure water with an eluting buffer of 0.1% FA in 100% ACN with a 90 min linear gradient of 11 to 37% ACN/0.1% FA at a flow rate of 300 nl/min.
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were gradient eluted with a flowrate of 200nL/min into an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific) mass spectrometer ...
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bioRxiv - Neuroscience 2024Quote: ... 700 fmol of standard synthetic AQUA peptides per mg of protein amount (Technologies, Thermo Fisher Scientific, Waltham, USA) were added to the endogenous peptide mixture and peptides were dried in a centrifugal Savant SpeedVac (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... After purification using SepPak C18 columns (Waters; WAT054955) the peptides were TMT-labeled (Thermo Fisher Scientific; 90061;TH266884) and fractionated with the High pH Reversed-phase Fractionation Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide extraction was performed using the EasyPep™ MS Sample Prep Kits (Thermo Fisher Scientific Inc, Massachusetts, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The peptides were analyzed by an Orbitrap Fusion Lumos tribrid mass spectrometer with an ESI source (ThermoFisher Scientific) coupled online to the nano-LC and operated in positive polarity ...
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bioRxiv - Cancer Biology 2024Quote: ... suspended in Formic Acid (FA) 0.1% and dosed with the quantitative fluorometric peptide assay (ref. 23290, Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated by reversed-phase chromatography using a 50-cm MicroPac Nano C18 column (Thermo Fisher Scientific) with mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile ...
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bioRxiv - Microbiology 2024Quote: TMT-labelled tryptic peptides were subjected to HpRP fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher) equipped with a 2.1 mm internal diameter x 25 cm long ...
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bioRxiv - Microbiology 2024Quote: ... Peptide sequences were determined by matching protein databases with the acquired fragmentation pattern by Sequest (Thermo Fisher Scientific). All databases include a reversed version of all the sequences ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were analysed by liquid chromatography coupled to mass spectrometry (LC-MS) using a Easy1200 nLC (Thermo Scientific) coupled to a tribrid Orbitrap Eclipse with FAIMSpro mass spectrometer (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Tagged ribosomes were then eluted by incubating the beads with 200 µg/mL HA peptide (Thermo Fisher Scientific) for 15 minutes at 30°C with constant agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides directly sprayed into an Orbitrap Fusion Lumos mass spectrometer equipped with a nanoFlex ion source (ThermoFisher Scientific). Full scan MS spectra (350-1400 m/z ...
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bioRxiv - Synthetic Biology 2024Quote: LC-MS/MS analyses of the digested peptides were performed on an EASY-nLC 1200 (Thermo Fisher Scientific) coupled to a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Acidified eluted peptides were analyzed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) in the data-dependent mode ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were analysed by nano HPLC-MS/MS with a Ultimate 3000 nanoRSLC (Thermo Fisher Scientific, Darmstadt, Germany) operated in a two- column setup (2 cm PepMap C18 trap ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS before staining with primary antibodies diluted in blocking buffer and a mixture of 1:1000 of Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Invitrogen), 1:5000 Dapi (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed X3 with PBS and incubated in the dark for 60 minutes at RT with Alexa Fluor 594 goat anti-rabbit IgG (H+L) (1:500 dilution in blocking buffer) (Invitrogen). Coverslips were washed X3 with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μm thick vibratome sections were postfixed with 4% PFA for 15 min at RT followed by blocking in 10% normal horse serum (Gibco) and 0.2% Triton in PBS overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... we incubated the samples in blocking solution with fluorescent dye-conjugated secondary antibodies and the nuclear counterstain DAPI (ThermoFisher Scientific) if applicable at 4 °C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... Developmental Studies Hybridoma Bank) was used at 1:30 in blocking solution and goat α-mouse Alexa568 secondary antibody (Invitrogen) at 1:1,000 in blocking solution ...