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Citations for Thermo Fisher :
3451 - 3500 of 3690 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... genomic DNAs were eluted with 50 μl of double-distilled water supplemented with 1 μl of 10 U/μL RNase I (Thermo Fisher Scientific, USA) and stored at −80°C for later experiments.
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bioRxiv - Microbiology 2020Quote: ... DNA was purified by phenol-chloroform extraction followed by isopropanol-sodium acetate precipitation and then resuspended in 50 μl nuclease-free water (Thermo Fisher, #10977-023). Extracted DNA was further diluted to 10 ng/ul and subjected to qPCR targeting of the EBV BALF5 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... An aliquot of the tryptic digest (in 2 % acetonitrile/0.1% formic acid in water) was analyzed by LC/MS/MS on an Orbitrap Fusion™ Tribrid™ mass spectrometer (Thermo Scientific™) interfaced with a Dionex UltiMate 3000 Binary RSLCnano System ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were placed on coverslips and were washed with PBS and Milli-Q water and then were mounted on glass slides with ProLong Gold mounting medium (Thermo Fisher Scientific, P36934). Cells were analyzed with a Nikon C2 confocal microscopy system (Nikon).
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bioRxiv - Microbiology 2022Quote: ... The RNA was suspended in 50 μL of RNAse-free water and checked using a NanoDrop™ 2000 (Thermo Scientific, Waltham, MA, USA). We kept the tubes containing the precipitate material with TRIzol® for DNA extraction conducted following the manufacturer’s protocol to confirm via PCR that mosquitoes had acquired infection after feeding on the infected canary.
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Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanismbioRxiv - Biophysics 2022Quote: NanoLC-MS/MS analysis was performed using a nanoAcquity Ultra-Performance-LC (Waters, Manchester, UK) coupled to a Q-Exactive Plus Orbitrap mass spectrometer (Thermo Scientific, Illkirch, France). Peptides were trapped on a nanoACQUITY UPLC precolumn (C18 ...
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bioRxiv - Biochemistry 2022Quote: ... Data were acquired using a NanoAcquity UPLC (Waters Corporation, Milford, MA) coupled to an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer ...
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bioRxiv - Systems Biology 2022Quote: Identification and quantification of nucleotides were performed using an ACQUITY UPLC chromatography system (Waters, UK) coupled online to a high-resolution Orbitrap mass spectrometer (Q-Exactive Focus, Thermo Fisher Scientific, Germany). For chromatographic separation ...
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bioRxiv - Plant Biology 2021Quote: ... Digested samples were diluted in distilled water at 0.65% (w/v) HNO3 and analyzed using an iCAP RQ quadrupole mass instrument (Thermo Fisher Scientific GmbH, Germany). Elements (24Mg ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples were eluted in 50-100 µl DES water and DNA concentrations were determined using the Qubit™ dsDNA HS Assay Kit (Invitrogen, life technologies) on Qubit®2.0 (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... The isolated RNA concentration was calculated and normalized with RNase-free water and reverse transcribed into cDNA using SuperScript II Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA, USA). After 10-fold dilution ...
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bioRxiv - Microbiology 2022Quote: ... as per the manufacturers’ instructions (Waters P/N WAT023590 for the pilot study; Thermo Scientific P/N 60109-001 for the larger study). The total eluate was dried completely using a vacuum concentrator (Savant SpeedVac or Eppendorf concentrator ...
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bioRxiv - Neuroscience 2022Quote: ... the distilled water was absorbed and a drop of the neuronal tracer rhodamine (1% in distilled water, Dextran, Tetramethylrhodamine, 3000MW, Anionic, Lysine Fixable, Thermo Fisher, Massachusetts, US) was applied and covered with vaseline to avoid dehydration in a total of 28 adult insects ...
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bioRxiv - Immunology 2022Quote: ... The pellets were dried at 37°C on a heat block followed by resuspending the pellets in 100 μL water (RNA) or TE buffer (DNA) (Thermo Fisher, Cat# 12090015). Tubes were placed back in the 37°C heat block and were shaken at 500 rpm for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... acetic acid in sterile water for 10 min then measured at an absorbance of 550 nm (Abs550nm) using a Multiskan Spectrum microplate reader (Thermo Fisher Scientific, USA). Each replicate’s biofilm capacity was measured in 7 technical replicates.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... This primer mix was further diluted with 0.9 µL of UltraPure water then combined with 6 µL of PowerUp™ SYBR™ Green (ThermoFisher, Waltham, USA) following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... eluted in 10 μl water and its quality was verified by ds-DNA concentration measurement with Qubit ds-DNA High Sensitivity assay (Thermo Fisher Scientific, USA) and BioAnalyzer (Agilent 2100 ...
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bioRxiv - Biochemistry 2020Quote: ... Urine metabolite separation and analysis was conducted using a Waters ACQUITY H-class LC system coupled with a LTQ-Orbitrap Velos Pro mass spectrometer (Thermo Fisher Scientific, MA). The following 18-min gradient on a Waters HSS C18 column (3.0 × 100 mm ...
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bioRxiv - Plant Biology 2020Quote: ... Resulting peptides were purified using Sep-Pak Vac tC18 100 mg cartridge (Waters, Milford, MA, USA), as described previously (Groen et al., 2013) completely dried in a Speed Vac concentrator (Thermo Scientific, Bremen, Germany) and analysed by tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Pathology 2021Quote: ... from the individuals of interest were recovered from the 42 °C water bath (without Sta-On tissue-adhesive) using Polysine® Slides (Thermo Fisher Scientific) and left to dry for 24 hours ...
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bioRxiv - Pathology 2021Quote: ... For each tumor sample 1000 ng of cDNA was diluted to 55 uL with nuclease-free water and 55 uL of TaqMan gene expression master mix was added (Life Technologies, cat # 4352042). A total of 100 uL of sample was added to each of the 8 ports on a single card ...
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bioRxiv - Molecular Biology 2021Quote: The mycelia were harvested and washed with cold water followed by freezing in liquid nitrogen and added 1ml TRIzol (Ambion, cat. no. 135405) then stored at −80 L until use ...
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bioRxiv - Developmental Biology 2021Quote: ... The temperature of the water flowing through the imaging chamber was measured using a digital thermometer (Fisher Scientific Dual Thermometer 15-077-26) and maintained between 26-28° C by adjusting the temperature of the heat block.
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bioRxiv - Genomics 2021Quote: ... and 3.8 μL of water) to determine the additional number of PCR cycles needed for optimal cDNA amplification (Applied Biosystems QuantStudio 7 Flex). We then prepared PCR reactions per total number of barcoded beads collected for each sNucDrop-seq run ...
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bioRxiv - Cell Biology 2021Quote: ... which was diluted from a stock 10mM EdU in distilled water from the Invitrogen Click-iT Edu Alexa Fluor 488 imaging kit (Invitrogen, cat. no. C10338). Worms were washed two times in PBS + 0.1% TritonX for 1-2 minutes each then plated onto either NGM or NGM with 1mM or 10mM auxin plates ...
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bioRxiv - Neuroscience 2021Quote: ... Fish were euthanized by immersion in ice water and pituitaries were collected and stored at −80 °C in 300 μl of TRIzol® (Invitrogen, Carlsbad, USA) with 6 zirconium oxide beads (Bertin Technologies ...
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bioRxiv - Immunology 2020Quote: ... NPs were first dispersed in milliQ water (1:100 dilution) and sonicated for 15 min in a bath sonicator (Thermo Fisher Scientific, USA). Dispersed samples were further processed following the procedure as mentioned above for OMVs ...
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bioRxiv - Microbiology 2020Quote: The fungal culture was dispensed in 100 mL distilled water (1:10 ratio) and shaken on a rotary shaker (MaxQ 2000 Open-Air Platform Shaker, Thermo Fisher Scientific, USA) at 240 rpm at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were eluted into 14 μl nuclease-free water and the RNA concentration was measured using Qubit HS RNA assay (Thermo Fisher Scientific #Q32852). Samples were stored at −20°C ...
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bioRxiv - Bioengineering 2021Quote: ... Rapamycin and PEG-PCL were dissolved in tetrahydrofuran and added drop by drop into MillQ water under ultrasonication (Sonic Dismembrator Model 500, Fisher Scientific, Waltham, MA) at 20% amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was removed and RNA pellets dried for 10 minutes prior to reconstitution in Ultrapure DNAse/RNAse-free distilled water (Thermo Fisher Scientific, 10977023) supplemented with DTT (1mM ...
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bioRxiv - Molecular Biology 2023Quote: ... a 15-μg aliquot of the trypsin and GluC HeLa digest was loaded onto an Acquity UPLC M-Class CS C18 column (Waters, Etten-Leur, Netherlands) for offline reversed-phase low pH fractionation using a Dionex Ultimate 3000 HPLC system (Thermo Scientific, Bremen, Germany). Peptides were loaded using buffer A (0.1% FA in water ...
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bioRxiv - Developmental Biology 2023Quote: ... Separation was accomplished by reversed phase chromatography with an Acquity UPLC HSS T3 (Waters) on a Vanquish™ Flex Quaternary UHPLC system (Thermo Fisher Scientific). Mass spectrometry was performed on a Quantiva™ triple quadrupole mass spectrometer interfaced with an H-ESI electrospray source (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... The larvae were washed 2 additional times with 40 mls of sterile water followed by 3 washes with 40 mls of sterile PBS (Gibco™ PBS [10X]). Larvae were counted under a dissecting microscope using a McMaster slide and stored in vented U-shaped 275 ml tissue culture flasks (Polystyrene tissue culture flasks with vented caps ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA pellets were dissolved in an appropriate volume of RNase free water (supplemented with 0.4 U/μl RNase inhibitor [RiboLock, Thermo Scientific, Cat.-No. EO0384]). RNA concentration was measured using NanoDrop (PeqLab).
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 50 uL of water and transferred into 9 mm Clear Glass Screw Thread Vials (1.7 mL Tapered Base,Thermo Scientific™ SKU C40009) with Vial Caps (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2023Quote: ... at 400 nL/min with a gradient of 4-30% with mobile phase B (Mobile phases A: 0.1% FA, water; B: 0.1% FA, 80% Acetonitrile (Thermo Fisher Scientific Cat No: LS122500)) over 160 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Trapping was performed at 20 μL/min for 2 min in loading solvent A (0.1% TFA in water/ACN (99.5:0.5, [v/v]) on a 5 mm trapping column (Thermo Fisher Scientific, 300 μm internal diameter). After flushing from the trapping column ...
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bioRxiv - Neuroscience 2023Quote: ... The combined lower layer was then washed with 300 μl of 1 M KCl followed by 300 μl of water and vacuum dried completely (Savant SpeedVac SPD130DLX vacuum concentrator, Thermo Fisher Scientific, USA) to obtain the dried lipid extract.
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bioRxiv - Genomics 2023Quote: ... were thawed in a 37 °C water bath for 3 minutes and then mixed with 37 °C thawing media containing: RPMI Medium 1640 (Life Technologies #11875-085) supplemented with 5% heat-inactivated fetal bovine serum (Life Technologies #16000044) ...
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bioRxiv - Microbiology 2023Quote: ... Cells in coverslips were rinsed with milli-Q water and mounted on glass slides using Prolong Gold anti fade mounting medium (Thermo Fisher Scientific, P101144) and stored at 4°C in the dark until imaging.
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bioRxiv - Bioengineering 2023Quote: ... 500 ng of cDNA were combined into a solution with 55 μL of nuclease free water and 55 μL TaqMan Universal PCR Master Mix (Thermo Fisher cat. 4305719). The combined solution was loaded into the gene array cards ...
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bioRxiv - Immunology 2023Quote: ... were pre-wet with 35% ethanol in sterile MiliQ water for 1 min and coated with 5 μg/mL of monoclonal mouse anti-pig IFN-γ antibody (clone P2F6, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... dissolved in 50 μL 0.1% formic acid and analyzed after a 1 h run on a 0.075 mm x 250 mm C18 Waters CSH column interfaced with an Orbitrap Eclipse mass spectrometer (Thermo Scientific, Waltham, Massachusetts, US). The instrument operated in data-dependent acquisition mode to perform bottom-up proteomics and the generated data were analyzed with Mascot (Matrix Science ...
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bioRxiv - Neuroscience 2022Quote: ... RNA samples were resuspended in a final volume of 12 μl of nuclease-free water and quantified by absorbance at 260 nm in a spectrophotometer (NanoDrop 2000, Thermo Fisher Scientific, USA). One microgram of total DNase-treated RNA was reverse-transcribed in a 20-μl reaction volume using M-MLV (Thermo Fisher Scientific ...
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Tryptophan specialized metabolism and ER body-resident myrosinases modulate root microbiota assemblybioRxiv - Plant Biology 2022Quote: The LC-MS system consisted of UPLC with a photodiode-array detector PDAeλ (Acquity System; Waters) hyphenated with a high-resolution QExactive hybrid MS/MS quadrupole Orbitrap mass spectrometer (Thermo Scientific, http://www.thermofisher.com/). Chromatographic profiles of metabolites and the quantitative measurements were obtained using water acidified with 0.1% formic acid (solvent A ...
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bioRxiv - Biochemistry 2024Quote: ... Dried extract was reconstituted in 1 mL of LC-MS-grade water and desalted by Sola HRP SPE 30mg/2MI 96-well plate 1EA (Thermo Scientific #60509-001). Desalting cartridges were cleaned three times with 1 mL of methanol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Deionized Milli-Q water up to a resistivity of 18 MΩ L cm was purified with a purification system (Barnstead EASYpure RoDi ultrapure water purification system, Thermo scientific, Ohio, USA).
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bioRxiv - Molecular Biology 2024Quote: ... samples were washed with ultrapure water and then negatively stained for 2 min using VitroEase™ Methylamine Tungstate Negative Stain (Thermo Scientific Co.) and allowed to dry for 15 min ...
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bioRxiv - Cell Biology 2024Quote: An aliquot of the tryptic digest (in 2 % acetonitrile/0.1% formic acid in water) was analyzed by LC/MS/MS using an Orbitrap Fusion™ Tribrid™ mass spectrometer (Thermo Scientific™) interfaced with a Dionex UltiMate 3000 Binary RSLCnano System ...