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Citations for Thermo Fisher :
3201 - 3250 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cut out protein bands were digested with trypsin and processed by a short reverse-phase LC-MS/MS on a Orbitrap Fusion Lumos Mass Spectrometer (Thermo Fisher Scientific). Proteins were identified using Proteome Discoverer 2.4 software (Thermo Fisher Scientific).
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: Desalted peptides were separated by nanoscale C18 reverse-phase liquid chromatography performed on an EASY-nLC 1200 coupled to an Orbitrap FusionTM LumosTM mass spectrometer (Thermo Fisher Scientific). Elution was carried out for a total run time duration of 265min (THP-1 and sorted pCAF proteome ...
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bioRxiv - Molecular Biology 2023Quote: ... coupled in line with an EASY-Spray PepMap RSLC C18 reversed-phase column (50 cm x 75 μm, 2 μm particle size, Thermo Fisher Scientific). The column was heated to 35°C and equilibrated with solvent A ...
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bioRxiv - Neuroscience 2023Quote: ... Dopamine content in µM was analyzed by reverse-phase LC-MS/MS custom assay in combination with an ABSciex 4000 QTrap® tandem mass spectrometer (Applied Biosystems/ MDS Analytical Technologies ...
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bioRxiv - Cell Biology 2023Quote: For mass spectrometry analysis reversed-phase chromatography was performed on a Thermo Ultimate 3000 RSLCnano system connected to a Q ExactivePlus mass spectrometer (Thermo Fisher Scientific) through a nano-electrospray ion source ...
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bioRxiv - Biophysics 2023Quote: Membrane potential was assayed by growing yeast to exponential phase in CSM containing 0.4% glucose followed by staining with 200 nM TMRE (Thermo Fisher Scientific T669) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated using vacuum centrifugation and separated into eight fractions using Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (84868, Thermo Scientific) according to the manufacturer’s instructions for TMT-labelled peptides ...
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bioRxiv - Immunology 2023Quote: ... and 6.6 µl were analyzed by an Ultimate 3000 reversed-phase capillary nano liquid chromatography system connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). Samples were injected and concentrated on a trap column (PepMap100 C18 ...
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bioRxiv - Microbiology 2023Quote: ... Two-hundred fifty microliters of log-phase culture were used to inoculate 50 mL of medium without antibiotic in a conical centrifuge tube (Thermo Scientific, 339652).
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bioRxiv - Microbiology 2023Quote: Stationary-phase cells from the growth curve cultures were diluted 1:10 into 0.85X phosphate buffered saline (PBS; Life Technologies, 70011-069) and transferred onto 1% agarose pads with 0.85X PBS to control for osmolality ...
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bioRxiv - Microbiology 2023Quote: ... and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID, Acclaim PepMap 100, C18, 2 µm, Thermo Scientific, MA, USA). Chromatography solvents were (A ...
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bioRxiv - Biochemistry 2022Quote: ... The three fractions were analyzed by reverse-phase chromatography tandem mass spectrometry on an Easy-nLC 1000 UPLC interfaced with a QE-Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) as described above ...
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bioRxiv - Biochemistry 2022Quote: ... and analyzed by reverse-phase chromatography tandem mass spectrometry on an Easy-nLC 1000 UPLC interfaced with a Orbitrap-Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a 2%–90% acetonitrile in 0.1% formic acid gradient over 120 min at 300 nl/min ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 100 ng of total sarcomeric protein extract (per sample) was separated using a MAbPac™ Capillary Reversed Phase HPLC Column (Thermo Scientific, 1500 Å pore size ...
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bioRxiv - Biochemistry 2023Quote: ... at a flow rate of 30 µl/min and separated using Acclaim PepMap C18 reversed-phase nano-LC columns (length, 500 mm; inner diameter, 75 μm; particle size, 2 μm; pore size, 100 Å; Thermo Scientific) at a flow rate of 0.25 µl/min ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared by 9ul per sample and separated using the High pH Reversed-Phase Peptide Isolation Kit (Thermo Fisher Scientific) as directed ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 μl were used to perform reversed-phase chromatography on a Thermo Ultimate 3000 RSLCnano system connected to a QExactivePLUS mass spectrometer (Thermo Fisher Scientific) through a nano-electrospray ion source ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting 96 fractions were recombined in a non-contiguous manner into 24 fractions and desalted using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific) followed by subsequent mass spectrometry analysis ...
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bioRxiv - Biochemistry 2023Quote: Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Cancer Biology 2023Quote: ... and washed for 3min on a C18 reverse phase precolumn (3μm particle size, 100 Å pore size, 75 m inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6μm particle size ...
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bioRxiv - Cell Biology 2023Quote: ... Those peptides were divided into several fractions using the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and the chromatographic separation was performed using an EASY-Spray C18 reversed-phase nano LC column (PepMap RSLC C18, 2 μm, 100A 75 μm x 25 cm, Thermo Fisher Scientific). The nanoLC was operating at 300 nl/min flow rate with a gradient (6-40% in 95 min and 5 min hold at 100% ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 1 μg of peptides were separated in 1 h gradients with reverse-phase chromatography being performed with an EASY-nLC 1000 ultra-high pressure system (Thermo Fisher Scientific), which was coupled to a Q Exactive Mass Spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Online chromatography was performed using the reverse phase liquid chromatography Dionex 3000 UHPLC system coupled to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). Each fraction was separated on a 50 cm x 75 µm Easy-Spray analytical column (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 600 μl of the lower phase was collected into a fresh 1.5 ml tube and vacuum dried on Speedvac (Thermo Scientific. Cat# SPD140DDA) before storage at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... resulting peptides were transferred to a glass vial and 3 µl were used to perform reversed-phase chromatography on a Thermo Ultimate 3000 RSLC nano system connected to a QExactivePLUS mass spectrometer (Thermo Fisher Scientific) through a nano-electrospray ion source ...
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bioRxiv - Microbiology 2023Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Fisher Scientific) over a 150 min organic gradient ...
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bioRxiv - Biochemistry 2023Quote: ... The digested peptides were separated by online reversed-phase chromatography on an Agilent 1290 Ultra-high performance LC (UHPLC) or Dionex UltiMate 3000 (Thermo Fisher Scientific) coupled to a Thermo Scientific Orbitrap Fusion mass spectrometer ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Eluted phosphopeptide samples from both enrichments were pooled and fractionated into 14 fractions by increasing ACN concentration from 7 % to 50 % using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC) with a Dionex Ultimate 3000 UPLC system (Thermo Fisher Scientific) on a reversed-phase XBridge BEH C18 column (3.5 μm ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μl of each sample was applied to an Ultimate 3000 reversed-phase capillary nano liquid chromatography system connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). The samples were injected and concentrated on a PepMap100 C18 trap column (3 μm ...
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bioRxiv - Developmental Biology 2023Quote: Cells in S-phase were detected by EdU-incorporation using the Click-ItTM EdU Alexa FluorTM 647 Imaging Kit (C10340, Thermo Fisher Scientific) following the supplier instructions ...
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bioRxiv - Plant Biology 2023Quote: Vacuum-dried organic phases were processed using ultra-performance liquid chromatography (on a C8 reverse-phase column) coupled with Fourier transform mass spectrometry (Q exactive mass spectrometer; Thermo Fisher Scientific) in positive and negative ionization modes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Two microliters of each fraction was resolved on a nanocapillary reverse-phase column (75 µm internal diameter by 50 cm; PepMap RSLC C18 column, Thermo Fisher Scientific) at a flowrate of 300 nL/min using 0.1% formic acid/acetonitrile gradient system (2 to 22% acetonitrile in 110 min ...
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bioRxiv - Cell Biology 2023Quote: A two-step RNA isolation protocol was followed for total RNA extraction: Organic phase isolation was performed with TriReagent®/ bromo–3–chloropropane (BCP) (Thermo Fisher Scientific/Molecular Research Center ...
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bioRxiv - Cell Biology 2023Quote: ... The enriched phosphopeptides were dried down and fractionated according to manufacturer’s instructions using High pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, 84868) for a final 6 fractions and subjected to C18 StageTip desalting prior to MS analysis.
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bioRxiv - Biochemistry 2023Quote: ... Dried peptides were resuspended in LC-Load and used to perform reversed-phase chromatography on a Thermo Ultimate 3000 RSLCnano system connected to a QExactivePLUS mass spectrometer (Thermo Fisher Scientific) through a nano-electrospray ion source ...
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bioRxiv - Molecular Biology 2023Quote: ... The peptide lysates were initially trapped on a C18-reverse phase trapping column (Acclaim PepMapTM 100, 75 μm × 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), and then separated on a C18-reverse phase analytical column (Double nanoViper™ PepMap™ Neo ...
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bioRxiv - Plant Biology 2023Quote: Oligosaccharides were purified by a two-step solid-phase extraction (SPE) using C18 (HyperSep C18-96, 50 mg bed weight; Thermo Fisher Scientific) and porous graphitic carbon (PGC ...
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bioRxiv - Molecular Biology 2023Quote: Obtained peptides were separated by nano-flow reversed-phase liquid chromatography coupled to a Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). Raw data files were processed for protein identification using MaxQuant ...
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bioRxiv - Microbiology 2023Quote: ... A 10 µl volume of phage lysate was added to a log-phase culture of the Klebsiella host strain in LB supplemented with 5 mM CaCl2 (Thermo Fisher Scientific) and 5 mM MgCl2 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Peptides in the compiled sample were fractionated (8 fractions) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat: 84868; Thermo Fisher Scientific). Prior to liquid chromatography–mass spectrometry (LC-MS ...
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bioRxiv - Cancer Biology 2023Quote: ... and the labelled samples were pooled prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Fisher Scientific). Fractions were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... and purified by solid-phase extraction (SPE) using HyperSep SPE columns (500 mg/10 mL, 40–60 μm, 70 Å; Thermo Scientific). FAHFAs were eluted with ethyl acetate and dried (24) ...
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 µl were analyzed by a Ultimate 3000 reversed-phase capillary nano liquid chromatography (LC) system connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). Samples were injected and concentrated on a trap column (PepMap100 C18 ...
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bioRxiv - Neuroscience 2023Quote: ... The TMT labeled peptide pools were separated into eight fractions with the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific), evaporated ...
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bioRxiv - Cell Biology 2024Quote: Peptides were analyzed by reverse-phase chromatography-tandem mass spectrometry on an EasyLC1100 UPLC interfaced with an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a 0%–100% acetonitrile in 0.1% formic acid gradient over 90 minutes at 300 nl/minute ...