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Citations for Thermo Fisher :
3251 - 3300 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Peptides were analyzed by reverse-phase chromatography-tandem mass spectrometry on an EasyLC1100 UPLC interfaced with an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a 0%–100% acetonitrile in 0.1% formic acid gradient over 90 minutes at 300 nl/minute ...
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bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The digested peptides were analyzed by nanoflow reverse-phase LC followed by tandem MS using a Q Exactive hybrid mass spectrometer (Thermo Fisher Scientific). The capillary reverse-phase HPLC-MS/MS system was composed of a Dionex U3000 gradient pump equipped with a VICI CHEMINERT valve ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were analyzed by reverse-phase chromatography-tandem mass spectrometry on an EasyLC nano HPLC interfaced with a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a 0%–100% acetonitrile in 0.1% formic acid gradient over 120 min at 300 nl/min ...
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bioRxiv - Physiology 2024Quote: ... Pictures of the wound area were taken before stimulation and 16 hours thereafter on a phase-contrast microscope (EVOS XL Core; Thermo Fisher Scientific). The width of the scratch was measured and quantified by NIH ImageJ software.
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bioRxiv - Biochemistry 2024Quote: ... at 300 nL/min was used to elute peptides from the capillary reverse-phase column (0.075 mm × 150 mm, Acclaim Pepmap 100® C18, Thermo Fisher Scientific). Eluted peptides were electrosprayed online at a voltage of 1.8 kV into the Orbitrap Elite mass spectrometer ...
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bioRxiv - Microbiology 2024Quote: TMT labeled peptides in each fraction were analyzed by nanoflow reverse phase chromatography coupled with tandem mass spectrometry (nLCMS/MS) on an Orbitrap-Fusion Lumos mass spectrometer (Thermo Fisher Scientific) interfaced with an EasyLC1000 UPLC ...
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bioRxiv - Microbiology 2024Quote: ... and 2 µl were analysed by an Ultimate 3000 reversed-phase capillary nano liquid chromatography system connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). Samples were injected and concentrated on a trap column (PepMap100 C18 ...
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bioRxiv - Microbiology 2024Quote: ... Procyclic of Cas9 and LmexNAT10-/+ cell lines were collected at logarithmic growth phase and incubated with 100 µM 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific A10044) for different periods of time (ranging from 30 min to 1.25 h ...
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bioRxiv - Microbiology 2024Quote: TMT labeled peptides in each fraction were analyzed by nanoflow reverse phase chromatography coupled with tandem mass spectrometry (nLCMS/MS) on an Orbitrap-Fusion Lumos mass spectrometer (Thermo Fisher Scientific) interfaced with an EasyLC1000 UPLC ...
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bioRxiv - Cell Biology 2024Quote: ... reversed-phase chromatography was performed on a Thermo Ultimate 3000 RSLCnano system connected to a Q-ExactivePlus mass spectrometer (Thermo Fisher Scientific) through a nano-electrospray ion source ...
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bioRxiv - Microbiology 2024Quote: ... was added to upper aqueous phase and centrifuged at 1500 x g for 5 minutes at 4°C.The upper aqueous layers were transferred to new MaXtract High-Density phase-lock tubes and incubated with RNase A at 25 µg mL−1 (EN0531, Thermo Fisher Scientific) at 150 r.p.m ...
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bioRxiv - Immunology 2024Quote: ... using a C18 reversed-phase column set at 35°C (75 μm i.d.× 150 mm, Pep Map™ RSLC C18, 2μm, 100Å, Thermo Fisher Scientific, ES904) connected to a Q-Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of the resuspended peptides were analyzed by reversed-phase nano–LC-MS/MS using a nano-Ultimate 3000 LC system and a Lumos Fusion mass spectrometer (Thermo Fisher Scientific). Flow rates were 400 nl/min ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Fisher Scientific, UK). In brief ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were separated by reverse phase HPLC on a Hypersil Gold C18 column (150 × 2.1 mm, 3 μm particle size, 175 Å pore size, Thermo Fisher Scientific) using a linear gradient (2-50% solvent B in 15 min) ...
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bioRxiv - Microbiology 2024Quote: ... The TMT-labeled peptide samples were loaded (2 µl) onto a C18-reversed phase Easy Spray column (50 cm long, 75 µm inner diameter, 2 µm particles; Thermo Fisher Scientific) with 100% nano buffer A ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... Pooled TMT-labelled peptides were cleaned and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to manufacturer’s instruction for TMT experiments ...
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bioRxiv - Systems Biology 2024Quote: ... Pictures of the wound area were taken at times 0 h and 16 h post migration using a phase-contrast microscope (EVOS XL Core; Thermo Fisher Scientific). The width of the scratch was measured and quantified by NIH ImageJ software.
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bioRxiv - Microbiology 2024Quote: The TMTpro-labeled peptides were fractionated into 12 concatenated fractions by reversed-phase chromatography in high pH buffers on a Dionex UltiMate 3000 (Thermo Fisher Scientific) equipped with a nanoEasy M/Z Peptide ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... TMT-labeled samples were fractionated into 8 fractions prior to LC-MS/MS analysis using the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific), following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... the ferricrocin concentration of the lower aqueous phase was measured at a wavelength of 434 nm using a nano spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were desalted on a reverse-phase PepMap 100 C18 μ-precolumn (5 mm, 100 Å, 300 mm i.d. × 5 mm, Thermo Scientific) and separated on a 50 cm EasySpray column (75 mm ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL of mid-log phase strain cultures (OD600 = 0.1, 50 μL in 96-well plates of Corning, Thermo Scientific Varioskan Flash) were stabbed into the plates containing modified Hv-Ca + 0.4% agar motility medium supplemented with additional trace elements ( per liter ...
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bioRxiv - Neuroscience 2024Quote: ... two µL of each sample was injected on a reversed-phase HPLC C18 column (Accucore C30 100 × 2.1 mm, 2.6 µm, Thermo Scientific, Waltham, MA, USA) using an Ultimate 3000 HPLC system (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Mass spectrometric analysis-chromatographic separation and label-free quantification: Tryptic peptides were separated by reverse phase nano-HPLC using an Ultimate 3000 RSLCnano System (Thermo Fisher Scientific) with a uPAC Trapping column (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting 96 fractions were recombined in a non-contiguous manner into 24 fractions and desalted using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific) followed by subsequent mass spectrometry analysis.
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bioRxiv - Cancer Biology 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Biochemistry 2024Quote: Peptide samples were separated using online reversed-phase nanoscale capillary LC on a Dionex UltiMate 3000 nanoRSLC chromatography system (Thermo Fisher Scientific) and analysed by electrospray ionization tandem mass spectrometry (MS ...
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Cancer Biology 2024Quote: ... The contents of the 18 tubes were pooled together and dried under vacuum prior to peptide separation using the High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplified cDNA was cleaned with solid-phase reversible immobilization (SPRI) beads and then quantified using a Qubit® 2.0 fluorometer (ThermoFisher; Waltham, MA). For the preparation of 3’ gene expression dual index libraries ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was performed using the SuperScript II Reverse Transcriptase kit (Thermofisher). RT-qPCR was performed using Fast SYBR Green Master Mix (Thermofisher ...
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bioRxiv - Cancer Biology 2021Quote: ... using Gateway™ LR Clonase™ II Enzyme mix (Invitrogen, 11791-020), forming the vector pINDUCER20-PPARGC1A-3xFLAG.
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bioRxiv - Cell Biology 2020Quote: ... 300 ng were reverse transcribed using Superscript II reverse transcriptase (LifeTechnologies/Invitrogen) using random hexamers (LifeTechnologies/Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... 0.7 mM dNTPs with 10 U SuperScript II reverse transcriptase (Invitrogen, 18064022). The obtained cDNA products were purified using Mag-Bind Total Pure NGS beads (Omega Bio-Tek ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The cells were counted using Countess II Automated Cell Counter (ThermoFisher Scientific). The total live cell count/ml was recorded as the final number of cells and the growth rate was calculated as Nf = N0 2ηt ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from isolated RNA using SuperScript II Reverse Transcriptase (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and synthesized cDNA from the resulting mRNA with SuperScript II RT (Invitrogen), using a primer containing a unique molecular identifier (UMI ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA was reverse transcribed with Superscript II (Invitrogen). Real time PCR was performed using primers 5’-ACGTGATGAGGAGGTTCT-3’ (sense ...
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bioRxiv - Developmental Biology 2020Quote: ... First strand cDNA synthesis was carried out using SuperScript II RT (Invitrogen). Quantitative real-time PCR primers were designed so that one of each primer pair was exon/exon boundary spanning to ensure that only mature mRNA was amplified ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was reverse transcribed to cDNA using SuperScript II Reverse Transcriptase (Invitrogen) using the provided random hexamer primers ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were counted using the Countess II FL Automated Cell Counter (ThermoFisher). Routine light microscopy was performed using the EVOS FL Cell Imaging System (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... following the protocol of SuperScript II reverse transcriptase (Thermo Fisher Scientific #18064014). Primers were designed against Rora isoform ENSMUST00000113624 (fwd ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction mixture contained 1X Accuprime PCR buffer II (Invitrogen, Carlsbad, CA), 1 unit Accuprime HiFi taq ...
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bioRxiv - Genomics 2020Quote: ... Cell viability was assessed via Trypan blue staining (Countess II, ThermoFisher #C10228), with >90% viability required for both PRO-seq and ChIP-seq experiments.
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bioRxiv - Genomics 2020Quote: ... BP reaction was performed using the BP Clonase II enzyme mix (Invitrogen) according to the manufacturer’s instructions.