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Citations for Thermo Fisher :
3101 - 3150 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... with a Falcon II direct electron detector (Thermo Fisher Scientific, USA) at the National Research Centre “Kurchatov Institute” ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen, Waltham, MA) with the addition of Actinomycin D ...
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bioRxiv - Bioengineering 2024Quote: ... PCR conditions: 25 µL 2x Platinum Superfi II Mastermix (Thermo Fisher), 19 µL water ...
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bioRxiv - Cell Biology 2024Quote: ... 2023) using the Gateway LR Clonase II Enzyme Mix (Thermo Fisher). The resulting COG4-mAID-3myc pLenti plasmid was then transformed into Stbl3 competent cells following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... coupled to a nanoflow LC system (Easy nLC II, Thermo Scientific). Peptide fractions were loaded onto a PepMapTM RSLC C18 nanocapillary reverse phase HPLC column (75 µm × 25 cm ...
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GRASP55 Safeguards Proper Lysosome Function by Controlling Sorting of Lysosomal Enzymes at the GolgibioRxiv - Cell Biology 2024Quote: ... and converted to cDNA using Superscript II (#18064014, Thermo Fisher Scientific). For the construction of the GNPTAB expression vector pcDNA4/TO/hGNPTAB-Myc-His ...
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bioRxiv - Developmental Biology 2024Quote: ... and counted on the Countess II FL Automated Cell Counter (Invitrogen). For hESCs and cyiPSCs 1.25 x 105 cells/cm2 and for mEpiSCs 6.25 x 104 cells/cm2 were seeded onto Matrigel coated plates in UPPS media supplemented with 10 µM Y-27632 (R&D Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μl of Super Script II RT from Invitrogen (18064-014) was added and incubated for 50 minutes at 42°C ...
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bioRxiv - Biophysics 2024Quote: Sf21 cells were cultured in SFM Sf-900 II medium (Gibco) at 28 °C to a density of 4.5×106 cells/ml and then were co-infected with GPBAR1 ...
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bioRxiv - Cell Biology 2023Quote: ... Muscles were minced with a sterile razor blade and transferred into digestion medium containing collagenase II and dispase II (Fisher Scientific, 17-101-015; 17-105-041) for enzymatic dissociation of mononuclear cells from muscle fibers in a rotating water bath at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were subjected to reversed-phase ion-pairing chromatography coupled by negative mode electrospray ionization to a stand-alone orbitrap mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... for 120min at 300nl/min was used to elute peptides from the capillary reverse-phase column (0.075 x 250mm, Pepmap®, Fisher Scientific, 164941). Data were acquired using the Xcalibur software (version 4.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... ONs were purified using preparative-scale reverse-phase high-performance liquid chromatography (HPLC) on a 250×4.0 mm Hypersil C18 column (Thermo Fisher Scientific; USA) with detection at λ=260 nm and a linear 7.5–25% acetonitrile gradient in 0.1 M ammonium acetate buffer over 45 min at 50 °C ...
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bioRxiv - Biochemistry 2022Quote: ... approximately 0.15 μg of peptides were run by online reversed phase chromatography on an Agilent 1290 UHPLC or Dionex UltiMate 3000 (Thermo Fisher Scientific) coupled to a Thermo Scientific Orbitrap Fusion mass spectrometer ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides were separated by a reversed-phase analytical column (PepMap RSLC C18, 2 μm, 100 Å, 75 μm X 25 cm) (Thermo Scientific). Flow rate was set to 300 nl/min at a gradient starting with 3% buffer B (0.1% FA ...
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bioRxiv - Molecular Biology 2020Quote: ... Oil red O and Alizarin Red S stained cells were imaged using phase contrast microscopy with an EVOS XL Core Cell Imaging System (Thermo Fisher Scientific).
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A Novel Mistranslating tRNA Model in Drosophila melanogaster has Diverse, Sexually Dimorphic EffectsbioRxiv - Molecular Biology 2021Quote: ... Peptides were separated by reverse-phase chromatography and online analyzed on a hybrid quadrupole orbitrap mass spectrometer (Orbitrap Exploris 480; Thermo Fisher Scientific) operated in data-dependent acquisition mode as described in Berg et al ...
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bioRxiv - Systems Biology 2020Quote: ... Up to 1mg peptide was fractionated by reverse-phase high pressure liquid chromatography (HPLC) on an Dionex UltiMate 3000 HPLC system (Thermo Scientific, USA) equipped with an XBridge® BEH C18 Sentry Guard Cartridge pre-column (130Å ...
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bioRxiv - Synthetic Biology 2020Quote: Five independent 10x phase contrast images were taken for each condition on each day using an EVOS FL inverted fluorescence microscope (Invitrogen, model AMF4300). Cell diameter (in points ...
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bioRxiv - Biochemistry 2020Quote: ... The peptides were then separated on a reversed-phase C18 analytical column (HPLC column Acclaim® PepMap 100, 75 μm I.D., 50 cm, 3 μm pore size; Thermo Fisher Scientific) with a gradient of 4 - 90% B over 70 min at a flow rate of 300 nl min−1 ...
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bioRxiv - Biochemistry 2020Quote: ... the peptides were first loaded onto a reversed-phase C18 pre-column (μ-Precolumn C18 PepMap 100, C18, 300 μm I.D., particle size 5 μm pore size; Thermo Fisher Scientific). 0.1% formic acid (v/v ...
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bioRxiv - Immunology 2021Quote: TMT-labelled tryptic peptides were subjected to pH reversed-phase (HpRP) fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher Scientific) equipped with a 2.1 mm internal diameter (ID ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were separated by nano-flow reversed-phase liquid chromatography coupled to a Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) using HCD fragmentation ...
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bioRxiv - Bioengineering 2020Quote: ... HTM cell morphology and growth characteristics were monitored by phase contrast microscopy using an LMI-3000 Series Routine Inverted Microscope (Laxco; Thermo Fisher Scientific). Once confluent ...
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bioRxiv - Bioengineering 2020Quote: ... Both methanol-treated media samples and intracellular metabolite extracts were analyzed by reversed-phase ion-pairing liquid chromatography (Vanquish UPLC; Thermo Fisher Scientific) coupled to a high-resolution orbitrap mass spectrometer (Q-Exactive plus Orbitrap ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were separated by nano-flow reversed-phase liquid chromatography coupled to a Q Exactive Hybrid orbitrap mass spectrometer (Thermo Fisher Scientific). The peptides were trapped onto a C18 PepMap 100 pre-column (inner diameter 300 mm × 5 mm ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm ...
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bioRxiv - Plant Biology 2020Quote: ... Vacuum-dried organic phases were processed using ultra-performance liquid chromatography (on a C8 reverse-phase column) coupled with Fourier transform mass spectrometry (exactive mass spectrometer; Thermo Fisher Scientific) in positive and negative ionization modes ...
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bioRxiv - Microbiology 2020Quote: Phase contrast and fluorescence microscopy was done on cells using Fisherbrand™ frosted microscope slides (Thermo Fisher Scientific Cat# 12-550-343) and 22 mm x 22 mm ...
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bioRxiv - Systems Biology 2021Quote: ... coupled with a customized LC system as previously described.15 The peptide fractions from high pH reversed-phase LC fractionation were analyzed an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled with a similar customized LC system ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were injected into a DIONEX UltiMate 3000 high-performance liquid chromatography (HPLC) system using a C18 reverse-phase Hypersil GOLD column (3 µm pore size, 150×4.60 mm; Thermo Fisher Scientific, Germany). The column oven temperature was set to 40 °C and UV-VIS absorbance spectra at 190-400 nm ...
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bioRxiv - Neuroscience 2020Quote: High pH reversed-phase peptide fractionation kit was used to perform desalting and fractionation as per manufacturer’s protocol (ThermoFisher, Cat. No. 84868). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... consisting of a reversed-phase trap column (Acclaim PepMap100 C18, 5 μm, 100 Å, 300 μm i.d. × 5 mm, Thermo Fisher Scientific) and a reversed-phase analytical column (30 cm ...
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bioRxiv - Molecular Biology 2021Quote: Cells were imaged both at low and high confluency using the EVOS FL Auto imaging system in phase contrast with a 40x and 400x objective (ThermoFisher Scientific, AMEP4680). Cell phenotypes and cell sheet patterns were characterised for all species and compared to human cells ...
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bioRxiv - Biophysics 2020Quote: Phase diagrams of non-phosphorylated and phosphorylated NSARS-CoV-2 at different concentrations were determined using a NanoDrop spectrophotometer (ThermoFisher Scientific, Invitrogen). Increasing concentrations of polyU (0 - 2 µM ...
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to reverse-phase LC separation on a 60-min gradient and analyzed on a Q Exactive HF-X (Thermo Fisher Scientific). Data-dependent fragmentation used collision-induced dissociation ...
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bioRxiv - Cancer Biology 2021Quote: The collected SCX fractions were reconstituted in 5% FA and 5% ACN (MS analysis solution) and analyzed in duplicate with 120 min linear gradients on an UltiMate 3000 RSLCnano reversed phase chromatographic platform (Thermo Fisher Scientific) coupled to a Q Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were separated on reversed phase C18 columns (trapping column: Acclaim PepMap 100, 300 μm × 5 mm, 5μm, 100 Å (Thermo Fisher Scientific); separation column ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were fractionated into a final eight fractions using a Pierce High pH reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868), according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... A portion of the peptides from the samples were pooled and fractionated using a Pierce High pH Reversed-Phase (HPRP) Peptide Fractionation Kit (Thermo Fisher Scientific) to generate a spectral library ...
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bioRxiv - Microbiology 2022Quote: ... The peptides were injected into a reverse phase C-18 column (PepMap RSLC C18, 2 μm, 100 Å, 75 μm × 50 cm, Thermofisher Scientific) and separated by a gradient flow of solvent B (0.1% formic acid in 80/20 acetonitrile/water ...
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bioRxiv - Cancer Biology 2022Quote: ... The TMT-labelled samples were subjected to fractionation using basic-pH reversed-phase liquid chromatography on a Dionex Ultimate 3000 HPLC system (Thermo Fisher Scientific) using a Gemini C18 column (250 mm × 3 mm ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting 96 fractions were recombined in a non-contiguous manner into 24 fractions and desalted using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific) followed by subsequent mass spectrometry analysis.
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bioRxiv - Cancer Biology 2022Quote: ... at 300 nL/min was used to elute peptides from the capillary reverse-phase column (0.075 mm × 150 mm, Acclaim Pepmap 100® C18, Thermo Fisher Scientific). Eluted peptides were electro-sprayed online at a voltage of 1.5 kV into the Q-Exactive+ mass spectrometer ...
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bioRxiv - Microbiology 2020Quote: The desalted peptides were analyzed by a reversed phase liquid chromatography electrospray ionisation tandem mass spectrometry (RP-LC-ESI-MS/MS) in an Ultimate 3000 nLC (Thermo Fisher Scientific) coupled to the Orbitrap Fusion® Lumos® Tribrid® (Thermo Fisher ...
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bioRxiv - Plant Biology 2021Quote: ... and an equivalent of 0.8–1.0 µg of peptides was separated using a reversed-phase column and analyzed on a Q-Exactive Plus or Q-Exactive HF spectrometer (Thermo Fisher Scientific). MaxQuant version 1.6.0.16 (Cox and Mann ...
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bioRxiv - Plant Biology 2021Quote: The dried aqueous phase was measured using ultra-performance liquid chromatography coupled to an exactive mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) in positive and negative ionization modes ...
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bioRxiv - Cancer Biology 2021Quote: ... and a heated (50 °C) reversed-phase analytical column (Acclaim PepMap RSLC C18, 2 µm, 100 Å, 75 µm × 150 mm, nanoViper, Thermo Scientific) coupled online with the MS ...
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bioRxiv - Cancer Biology 2021Quote: ... at 0.6X SPRI (solid-phase reversible immobilization) followed by a 1X SPRI and quantified using a Qubit dsDNA High Sensitivity assay kit (Thermo Fisher Q32854). Library preparation was performed using Nextera XT DNA tagmentation (Illumina FC-131-1096 ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated by reversed-phase chromatography using a Dionex U3000 HPLC coupled to a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a binary buffer system comprising 0.1% formic acid (buffer A ...