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Citations for Thermo Fisher :
3151 - 3200 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... coli strains DH5α and BL-21-AI were obtained from Invitrogen (Carlsbad, CA). Isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... coli colonies were grown at 37°C shaking in Miller’s lysogeny broth (Invitrogen) until reaching an OD600 of 0.1 when they were induced with a final concentration of 10 mM arabinose to express Csm1-6 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by second strand cDNA synthesis with Eschericcia coli DNA pol I (Invitrogen) and RNAse H (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The pET-28b clone was expressed in Rosetta (DE3) Escherichia coli cells (Invitrogen). The cells were grown to mid log phase ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3)pLysS (Thermo Fisher Scientific). 1-4 liters of cells were grown in Luria-Bertani (LB ...
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bioRxiv - Immunology 2022Quote: ... coli cells containing a baculovirus shuttle vector and helper plasmid (DH10Bac cells; ThermoFisher) to obtain colonies containing recombinant bacmids ...
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bioRxiv - Plant Biology 2022Quote: ... coli clones were first induced with 0.1 mM IPTG (Thermo Fisher Scientific, 15529019) at 16°C for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were performed using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen). Hfx ...
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bioRxiv - Microbiology 2022Quote: ... coli DNA digested with FastDigest HindIII and NotI (Thermo Fisher Scientific, MA, USA) was used as a template in ddPCR reactions optimized for 16S rRNA ...
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bioRxiv - Microbiology 2024Quote: ... coli strains were performed using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... Final products were electroporated into DH10ß electrocompetent Escherichia coli cells (Thermo Scientific, EC0113) and purified to achieve a highly diverse plasmid library
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bioRxiv - Plant Biology 2023Quote: ... Expression of 6His-CPK3 was induced in Escherichia coli strain BL21-AI (Invitrogen) with 0.2% (m/v ...
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bioRxiv - Plant Biology 2022Quote: ... coli BL21 cells using HisPur™ Ni-NTA Spin Columns (Thermo Fisher Scientific). Protein size and purity was assessed by SDS-PAGE (4-12% gradient gel ...
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bioRxiv - Biochemistry 2022Quote: ... coli DH5α competent cells according to the TOPO® Cloning reaction protocol (Invitrogen). Positive transformants were selected on LB-agar-ampicillin (50 μg.mL-1) ...
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bioRxiv - Microbiology 2023Quote: ... overnight Escherichia coli CAN-50 growth in Luria-Bertani (LB, ThermoFisher, Massachusetts, USA) broth equivalent to 109 CFUs was spun down at 16,000 xg ...
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bioRxiv - Bioengineering 2023Quote: ... coli expression were generated using Invitrogen GeneArt web service (Thermo Fisher Scientific, USA). iTerm-PseKNC30 was used for identification of transcriptional terminators ...
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bioRxiv - Biochemistry 2023Quote: ... coli using Strings DNA Fragments Synthesis Service (Thermo Fisher Scientific, Waltham, MA, USA) (Supplementary Data S3) ...
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bioRxiv - Molecular Biology 2023Quote: ... and transformed into One Shot TOP10 Chemically Competent Escherichia coli (ThermoFisher Scientific, www.thermofisher.com). The restriction enzyme-epitope-coding sequence fragment was excised by restriction enzyme digestion ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli and cultured them in LB Miller Broth (Thermo Fisher Scientific, Waltham, MA) with 100 μg/mL Carbenicillin (Gold Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... coli cells and were purified from extracts using glutathione agarose (#16100, Thermo Fisher) as described previously (61) ...
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bioRxiv - Microbiology 2023Quote: ... coli DH5ɑ were washed once in phosphate buffered saline solution (PBS; Thermo Scientific), and pelleted ...
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bioRxiv - Microbiology 2023Quote: ... coli was carried out using Invitrogen TRIzol Reagent (Thermo Fisher Scientific; Cat. # 15596018). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... coli cells was done with DreamTaq polymerase (Thermo Fisher Scientific GmbH, Dreieich, Germany). Phire Plant Direct PCR Master Mix (Thermo Fisher Scientific GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... coli was purified using the GenJET Plasmid Miniprep kit (Thermo Scientific, Waltham, USA). Chromosomal DNA from Hm ...
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bioRxiv - Genetics 2024Quote: ... coli OP50 topically spotted with 150µL of 0.5M ethylenediaminetetraacetic acid (EDTA) (ThermoFisher, AM9260G) or 150µL of water ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli bacterial transformation with Invitrogen XL-1 Blue competent cells (ThermoFisher, Carlsbad, CA) was used to isolate new DNA constructs ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligation mixture was then transformed into DH5α Escherichia coli (ThermoFisher Scientific, USA) grown on LB plates with 100 μM Ampicilin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Biochemistry 2020Quote: ... optiMEM (1X) reduced serum medium and heat-inactivated fetal bovine serum (HI-FBS) were from Gibco chemicals ...
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using a pcDNA 3.1-V5/His TOPO-TA cloning kit (Invitrogen K480001) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
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bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
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bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...