Labshake search
Citations for Thermo Fisher :
3301 - 3350 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
-
bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... Inserts were then subcloned into pCS2+ N’ HA tagged vectors that had been converted into Gateway® (Invitrogen) destination vectors ...
-
bioRxiv - Molecular Biology 2020Quote: ... at a 1:5000 dilution and endogenous LC3 was tagged with an LC3B/MAP1LC3B primary antibody (ThermoFisher, L10382) used at a dilution of 1:500 for 72 hours at 4°C ...
-
bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the HA-glmS-tagged parasite lines were coated on diagnostic slides (Epredia; Thermo Fisher Scientific; Waltham, US) and air-dried ...
-
bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
-
bioRxiv - Evolutionary Biology 2023Quote: Lamprey embryos were injected with MYC-tagged lamprey (pou5) or Xenopus (pou5f3.1, pou5f3.2, pou5f3.3) mRNA and a FITC dextran tracer (ThermoFisher AAJ6360622) into one blastomere at the two-cell stage at the same concentration used for injections in Xenopus ...
-
bioRxiv - Microbiology 2024Quote: ... goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001) was used ...
-
bioRxiv - Biochemistry 2024Quote: Construct containing Avi-tagged HormR/GAIN domains of ADGRL3 was expressed in High Five cells (Thermo Fisher, B85502) grown in Insect-Xpress medium (Lonza ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were either transduced to include a fluorescent reporter or fluorescently tagged using Hoechst (NucBlue Live ReadyProbes, Invitrogen) or general membrane cell tracker (Cellvue Claret Far Red ...
-
bioRxiv - Cell Biology 2023Quote: ... strep tagged APC/C was pre-incubated on ice with streptavidin conjugated Alexa Fluor 700 (ThermoFisher Scientific S21383) for 30 minutes ...
-
bioRxiv - Bioengineering 2024Quote: GFP-HUVECs were added to well 1 and GFP-HUVECs co-tagged with Hoechst (NucBlue Live ReadyProbes, Invitrogen) were added to wells 2-4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
-
bioRxiv - Biochemistry 2022Quote: Cloning of full-length N-terminal strepII-tagged hItln1 into the pcDNA4/myc-HisA vector backbone (Life Technologies) was described previously (14) ...
-
bioRxiv - Cell Biology 2023Quote: ... To visualise the cell surface with Alexa488-tagged wheat germ agglutin (Thermo Fisher Scientific, UK, 1:2000 dilution) an incubation was done prior to permeabilization ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genes encoding tagged and untagged SOSIP.664 Env homotrimers were expressed by transient transfection of Expi293 cells (ThermoFisher). Env heterotrimers were purified from co-transfections involving a 20:1 expression plasmid DNA ratio of untagged to tagged Env constructs ...
-
Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
-
bioRxiv - Molecular Biology 2023Quote: FLAG-tagged β-catenin variants were expressed in transient transfection assays using the pCDNA3.1 vector (Thermo Fisher Scientific). A plasmid expressing human β-catenin containing a S33Y mutation (pCDNA3-S33Y ...
-
bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5’ ends were synthesized by Thermo Scientific, while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... Tagged ribosomes were then eluted by incubating the beads with 200 µg/mL HA peptide (Thermo Fisher Scientific) for 15 minutes at 30°C with constant agitation ...
-
bioRxiv - Cell Biology 2024Quote: ... incubated with fluorescent-tagged anti-rabbit-Alexa 550 or anti-mouse-Alexa 488 secondary antibodies (Thermo Fisher Scientific) and DAPI for 1 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with plasmids encoding fluorescence-tagged PARP2 or XRCC1 using Lipofectamine 2000 (Invitrogen, Cat. 11668019) or Lonza 4D-Nucleofector™ X ...
-
bioRxiv - Neuroscience 2021Quote: ... The paraffin-embedded retina was stained with hematoxylin and eosin (H&E) (Thermo Fisher Scientific) to evaluate the cellular structure of the retina ...
-
bioRxiv - Neuroscience 2021Quote: ... cortical tissue was dissected from E15.5 embryos and kept on ice in Hibernate-E (GIBCO) supplemented with 2% B27 (GIBCO ...
-
bioRxiv - Developmental Biology 2022Quote: ... were maintained in SF900 media (Lif e Technologies) supplemented with 1x Antibiotic-Antimycotic (Life Technologies) at 25°C ...
-
bioRxiv - Microbiology 2020Quote: ... were removed from the final pool using a 2% E-gel size-select II (ThermoFisher). Quality of the pool was assessed with the Bioanalyzer DNA 1000 chip (Agilent Technologies ...
-
bioRxiv - Immunology 2019Quote: ... The concentration of E/S was measured using the Nanodrop 1000 spectrophotometer (Thermo Fisher Science) and aliquoted before storing at −80°C.
-
bioRxiv - Genetics 2019Quote: ... Samples were then pooled together and run on a 4% agarse E-Gel (Life Technologies), and the 140-160 nt length bands were excised and purified using MiniElute Gel Extraction Kit ...
-
bioRxiv - Systems Biology 2021Quote: R1/E mouse embryonic stem cells (mESCs) were cultured in DMEM (high glucose, pyruvate, Gibco) supplemented with 16 % FBS (Gibco) ...
-
bioRxiv - Neuroscience 2022Quote: ... Cortices of E-18 Wistar rat embryos were dissociated in trypsin with 0.25% EDTA (Gibco) and counted with a hemocytometer as described previously [32] ...
-
bioRxiv - Neuroscience 2021Quote: ... We dissociated cortices of E-18 Wistar rat enzymatically in trypsin with 0.25% EDTA (Gibco), followed by trituration ...
-
bioRxiv - Immunology 2019Quote: ... The concentration of E/S was measured using the Nanodrop 1000 spectrophotometer (Thermo Fisher Science) and aliquoted before storing at - 20°C.
-
bioRxiv - Cell Biology 2020Quote: ... Anti-E-cadherin (2.5 µg/mL, clone 36, BD Transduction Laboratories or SHE78-7, Invitrogen), anti-KI-67 (1:400 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR products were then run on E-Gels EX 2% Agarose (Cat# G402002) from Invitrogen and amplicons of about 586 bp were extracted using the Qiagen QIAquick Gel Extraction Kit (Cat# 28706) ...
-
bioRxiv - Molecular Biology 2021Quote: ... pronase E (0.4 mg/mL) and collagenase IV (0.8 mg/mL, Thermo Fisher Scientific, 17104019) (Step 2) ...
-
bioRxiv - Immunology 2020Quote: ... cut into 10 μm sections of thickness using a Shandon Cryotome E cryostat (Thermo Scientific), and mounted on Starfrost® adhesive slides (Knittel Glass) ...
-
bioRxiv - Microbiology 2021Quote: ... The FTHFS amplicons were purified by E-Gel® iBase™ Power System (Invitrogen 2012) and E-Gel® EX with SYBR® Gold II ...
-
bioRxiv - Cancer Biology 2020Quote: ... The amplified sequences were purified on E-Gel® EX 4% Agarose gels (ThermoFisher # G401004), and sequences representing RNA smaller than 200 nt were extracted from the gel ...
-
bioRxiv - Developmental Biology 2022Quote: ... Libraries were then run on an E-Gel EX 2% agarose gel (Cat. # G402022, Invitrogen) for 10 min using the E-Gel Power Snap Electrophoresis System (Invitrogen) ...
-
bioRxiv - Genomics 2022Quote: ... 23 libraries (Supplementary Table 1) underwent a second round of size selection (Invitrogen E-Gel) to remove excessive primer dimers ...
-
bioRxiv - Bioengineering 2022Quote: ... All PCR products were validated using E-Gel EX-Gels with 2% Agarose (Thermo Fisher).
-
bioRxiv - Cell Biology 2022Quote: ... resuspended in Williams Medium E (MilliporeSigma, Burlington, MA) with plating supplements (CM3000; Thermo Fisher Scientific) and plated at a density of 50,000 cells/cm2.
-
bioRxiv - Biochemistry 2022Quote: ... adherent HEK293T and LRRC8B,D,E-/- cells were grown in high glucose DMEM medium (Gibco), supplemented with 10% FBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... different CNS regions were dissected and kept in ice-cold Gibco Hibernate-E medium (ThermoFisher) until further processing ...
-
bioRxiv - Genomics 2022Quote: ... Libraries were then run on an E-Gel EX 2% agarose gel (Cat. # G402022, Invitrogen) for 10 min using the E-Gel Power Snap Electrophoresis System (Invitrogen) ...
-
bioRxiv - Genomics 2022Quote: ... we fill the sample wells of a 2% E-Gel EX Agarose Gels (ThermoFisher G401002) with 16 µL of Ultrapure water and 4 µL of qPCR reaction ...
-
bioRxiv - Neuroscience 2023Quote: ... The brains were removed quickly and placed into ice-cold Hibernate E (Thermo Fisher Scientific). The forebrain was dissected ...