Labshake search
Citations for Thermo Fisher :
3401 - 3450 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Plasmid expansion was performed in thermo-competent Escherichia coli (Omnimax T1 cells; Thermo Fisher) in lysogeny broth (LB ...
-
bioRxiv - Cell Biology 2021Quote: ... coli (K-12 strain), and Staphylococcus aureus (Wood strain, without protein A) (Molecular Probes), were washed 3 times with PBS by centrifugation and sonicated for 3 times at 50 KHz for 20 s ...
-
bioRxiv - Plant Biology 2022Quote: ... Vectors were transformed into Escherichia coli One Shot OmniMAX 2 T1 (ThermoFisher Cat# C854003). CRISPR-Cas9 expression vectors carrying gRNA_W or gRNA_F were transformed in Agrobacterium tumefaciens (GV3101) ...
-
bioRxiv - Molecular Biology 2024Quote: ... coli bacterial pellet using the Invitrogen™ PureLink™ RNA Mini Kit (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: Plasmid maintenance and amplification was carried out using Escherichia coli TOP10© (ThermoFisher Scientific). All PrP constructs were generated by standard PCR cloning techniques and are based on the coding region of mouse PRNP (GenBank accession number M18070 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... coli populations were dilution plated on LB + 0.5 % agar (Agar agar, ThermoFisher Scientific, India), incubated at 32 °C for 18 h ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Vectors were transformed into One Shot TOP10 chemically competent Escherichia coli (Thermo Fisher Scientific) cells as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: Plasmid maintenance and amplification was carried out using Escherichia coli TOP10© (ThermoFisher Scientific). All PrP constructs were generated by standard PCR cloning techniques and are based on the coding region of mouse PRNP (GenBank accession number M18070 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and transformed into One Shot TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific, www.thermofisher.com). Sequences were confirmed by Sanger sequencing ...
-
bioRxiv - Biophysics 2023Quote: ... coli BL21 (DE3) cells (44) and fluorescently labeled with AlexaFluor C5 maleimide (Molecular Probes) following the supplier’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... coli cultures were stained with CFSE (Cell-Trace CFSE Cell Proliferation Kit, Thermofisher, C34554) before infection of HCT116 and HT-29 cells at MOI 100 ...
-
bioRxiv - Microbiology 2023Quote: ... coli isolates were saved in cryovials containing Brucella broth (Thermo Fisher Scientific, Lenexa, KS) with 15% glycerol and subsequently stored at -80 °C for sequencing analysis.
-
bioRxiv - Molecular Biology 2023Quote: Single plasmids were chemically transformed into One Shot STBL3 chemically competent coli (Invitrogen C737303). Bacterial cultures were shaken at 225 rpm for one hour at 37°C and then plated on an ampicillin agar dish ...
-
bioRxiv - Neuroscience 2023Quote: Each plasmid was transformed into BL21 (DE3)pLysS chemically competent Escherichia Coli cells (Invitrogen, La Jolla ...
-
bioRxiv - Microbiology 2023Quote: ... coli-loaded paramecia were counted using an automated cell counter (Life Technologies Countess II) and a final concentration of 2*105 paramecia/mL in E3 medium was used to feed E ...
-
bioRxiv - Immunology 2023Quote: ... coli ATCC 25922 (3.75 x 106) were stained with Syto9 500 nM (Invitrogen/Thermo) and incubated with non-immune or TolC-immune serum (1:100 ...
-
bioRxiv - Microbiology 2024Quote: ... Escherichia coli and Escherichia fergusonii were grown in Lennox lysogeny broth (LB, Fisher Scientific) with aeration or on LB agar at 37°C ...
-
bioRxiv - Genetics 2024Quote: ... coli under appropriate conditions and purified using GeneJET plasmid miniprep kit (Thermo Fisher Scientific) using included instructions ...
-
bioRxiv - Genomics 2024Quote: ... Escherichia coli S17-1 was grown in Lennox L broth (Fisher Scientific, Hampton, NH) with aeration at 37° C ...
-
bioRxiv - Genomics 2024Quote: ... Escherichia coli S17-1 was grown in Lennox L broth (Fisher Scientific, Hampton, NH) with aeration at 37° C ...
-
bioRxiv - Biophysics 2021Quote: ... Control Drosophila melanogaster biotin-tagged kinesin-1 motors were labeled with streptavidin-conjugated Qdot 655 (Life Technologies, Carlsbad, CA) at a 1:4 motor:Qdot ratio as previously described [16 ...
-
bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
-
bioRxiv - Cell Biology 2020Quote: ... The pDONR201 clones were transferred into GFP or SFB tagged DEST vectors using the Gateway LR cloning system (Invitrogen). KDM5AΔPID deletion mutant was generated by PCR in pDONR201 vector following standard cloning method and then subcloned into Gateway destination vector derived from pcDNA5/FRT/TO plasmids containing the NLS and SFB- or GFP-tagged in N-terminal ...
-
bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
-
bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2020Quote: HEK293T cells grown on 100-mm dishes were cotransfected with Flag-tagged ASK1_KR and PKA or empty vector using Lipofectamine 2000 (Invitrogen). Twenty-four hours after transfection ...
-
bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...
-
bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
-
bioRxiv - Genomics 2020Quote: ... 20.25 μg 3×Flag-tagged dCas9 plasmid and 20.25 μg sgRNA plasmid were transfected into cells using Lipofectamine 3000 (Invitrogen). Two biological replicates were performed for each condition ...
-
bioRxiv - Cancer Biology 2021Quote: Control MCF-10A cells or cells expressing HA-tagged PEAK3 were cultured in -EGF medium (DMEM/F-12 (Gibco) supplemented with 0.5◻μg/ml hydrocortisone (Sigma) ...
-
bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
-
bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
-
bioRxiv - Biochemistry 2021Quote: N-terminal GST-tagged MBNL1 (amino acids 1-260)59 was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
-
bioRxiv - Biophysics 2021Quote: ... and the tagged SΔTM was purified by affinity chromatography using Ni-NTA agarose beads (Invitrogen™, Waltham, MA, USA) and size exclusion chromatography as described above.
-
bioRxiv - Microbiology 2021Quote: ... bacteria were tagged by Gfp or stained by cell membrane stain FM4-64 (10 µM final concentration, Molecular Probes) for 2 mins in the dark (without flow) ...
-
bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
-
bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
-
bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells at 40% confluence were transfected with 1 ug of GFP-tagged LRRK2 plasmid using Lipofectamine 2000 (Invitrogen, 11668019) as described for 12-15 hrs ...
-
bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
-
bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
-
bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... Purified A-C/EBP (30 - 40 μg) was tagged with Alexa Fluor 488 according to manufacturer’s protocol (Thermo fisher). Time lag expression of A-C/EBP protein was observed using EGFP tagged A-C/EBP plasmid (pCMV-A-C/EBP-EGFP ...
-
bioRxiv - Immunology 2019Quote: ... The labeled cells were counter-stained with a fluorophore-tagged secondary goat anti-rabbit IgG (H+L) antibody (Invitrogen), before acquisition on a LSR Fortessa (BD Biosciences) ...
-
bioRxiv - Biophysics 2020Quote: ... The C-terminally SBP-tagged DISP1-A or DISP1-A-NNN was captured using Streptavidin-agarose affinity resin (ThermoFisher). The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff ...
-
bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... captured dsRNA was detected by incubating with sulfo-tagged rat anti-mouse IgM antibody (Thermo Fisher Scientific, Waltham, MA) for 1h before washing and adding MSD GOLD Read Buffer A (Meso Scale ...
-
bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...