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Citations for Thermo Fisher :
3151 - 3200 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific, IL, USA) and a total of 100 μg of protein per sample was subjected for overnight trypsin digestion ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-mouse CLDN5 (Cat #34-1600, Thermo Fisher Scientific, Rockford, IL, USA) and Alexa 488-conjugated Phalloidin (Cat# A12379 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and integrity were evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, IL, USA), Qubit 2.0 Fluorometer together with the DNA High Sensitivity kit (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: Relative transcript levels of the inflammatory cytokines IL-1ß (ThermoFisher Scientific, Cat. # Mm00434228_m1), and TNFα (Cat ...
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bioRxiv - Cancer Biology 2021Quote: ... and Streptavidin Alexa Fluor 488 conjugate were purchased from Thermo Scientific (Rockford, IL). Aniline was from BeanTown Chemical Corporation (Hudson ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM EDTA and Halt Protease inhibitor cocktail (Thermo Scientific, Rockford, IL, USA). The cell lysates were fractionated using 4–12% NuPAGE gels (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... and 30 U/ml of interleukin-2 (IL-2; Thermo Fisher Scientific, PHC0026) at 37°C ...
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bioRxiv - Immunology 2021Quote: Cytokine concentration was measured by using IL-6 Mouse Uncoated ELISA Kit (Invitrogen) according to manufacturer’s instructions ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Blots were incubated with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, Rockford, IL) and exposed to Amersham Hyperfilm ELC (GE Healthcare) ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was assessed with a Nanodrop 2000 spectrophotometer (Thermo Scientific, IL, USA), by a Qubit 2.0 Fluorometer with the DNA High Sensitivity kit (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... HRP was activated using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL) and imaged with a FluorChem Q system (Protein Simple ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88 ...
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bioRxiv - Neuroscience 2022Quote: ... IL-6 (1:4-1:2 dilution, #KMC0061; ThermoFisher Scientific, Waltham, MA, USA), and NfL (1:4 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% non-essential amino acids (all Thermo Fisher Scientific, Rockford, IL, USA) at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... TNF alpha and IL-6 were quantified using the Procartaplex assays from Invitrogen. To assess functionality of KO in iPSC-derived myeloid cells ...
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bioRxiv - Microbiology 2024Quote: ... we used the Phusion™ Site-Directed Mutagenesis Kit (Thermo Scientific, Rockford, IL) with primers listed in SI Table S6 ...
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bioRxiv - Immunology 2024Quote: ... Wells were blocked with StartingBlock PBS Blocking Buffer (Thermo Scientific; Rockford, IL, USA) for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... CYP1B1 (rabbit polyclonal igG AB; #PA5-95277; 1:1000; Invitrogen, Rockford, IL, USA); secondary ...
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bioRxiv - Genomics 2023Quote: ... RNA purity was evaluated using a Nanodrop 2000 spectrophotometer (Thermo Scientific, IL, USA). DNA quantity was verified using a Qubit 2.0 Fluorometer and a broad range RNA kit (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... DNA purity was evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, IL, USA), DNA quantity with the DNA High Sensitivity kit on a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% non-essential amino acids (all Thermo Fisher Scientific, Rockford, IL, USA) at 37°C in a humidified atmosphere with 5% CO2 ...
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Disruption of DLL4/NOTCH1 Causes Dysregulated PPARγ/AKT Signaling in Pulmonary Arterial HypertensionbioRxiv - Cell Biology 2024Quote: ... Tissue sections were mounted with Prolong Diamond Antifade Mountant (ThermoFisher Scientific, Rockford, IL) and scanned using the Zeiss Axioscan Z1 (Zeiss ...
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bioRxiv - Immunology 2023Quote: ... and by the IL-27 Mouse ELISA kit (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and Alexa-Fluor 568-conjugated secondary antibodies were purchased from ThermoFisher (Rockford, IL). Polyinosinic:polycytidylic [poly (I:C)] ...
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bioRxiv - Microbiology 2023Quote: ... and Alexa-Flour 568-conjugated secondary antibodies were obtained from ThermoFisher (Rockford, IL). Human tumor necrosis factor-α (TNF-α ...
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bioRxiv - Microbiology 2023Quote: ... and quantified using Pierce 660nm Protein Assay Reagent (ThermoFisher Scientific, Rockford, IL, USA). Next ...
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bioRxiv - Genetics 2023Quote: ... containing Pierce protease inhibitor mini tablets (cat. A32953, Thermo Fisher Scientific, IL, USA) and PhosStop™ tablets (cat ...
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bioRxiv - Immunology 2023Quote: ... antibodies were combined with IL-33red in Iscoves’s Modified Dulbecco’s Medium (IMDM, Gibco) overnight before the treatment of the cells for 24 hours.
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bioRxiv - Cell Biology 2023Quote: ... IL-10 mRNA levels were determinated using gene specific primers (Hs00961622_m1; ThermoFisher Scientific). As reference the expression of housekeeper genes GAPDH (Hs02758991_g1) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PierceTM HA epitope tag antibody was obtained from Thermo Scientific (Rockford, IL, USA). The rabbit polyclonal phosphosite-specific µ-opioid receptor antibodies anti-pT370 (7TM0319B) ...
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bioRxiv - Cell Biology 2023Quote: ... After incubation with peroxidase-conjugated secondary antibody (Thermo Fisher Scientific, Rockford, IL, USA), signals were revealed with enhanced chemiluminescence (ChemiDoc MP ...
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bioRxiv - Cancer Biology 2024Quote: The total RNA was extracted with TRizol (Thermo Fisher Scientific, Rockford, IL, USA) following the manufacturer’s instructions and the concentration ...
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bioRxiv - Neuroscience 2024Quote: ... Bicinchoninic Acid (BCA) protein assay kit was obtained from Thermo Scientific (Rockford, IL). ProSignal Blotting Film was purchased from Genesee Scientific (El Cajon ...
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bioRxiv - Biochemistry 2024Quote: ... UHPLC/MS Optima acetonitrile was obtained from Fisher Scientific (Hanover Park, IL, USA).
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 594 conjugated secondary antibody (A-11058; Thermo Fisher Scientific, Rockford, IL), along with DAPI ...
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bioRxiv - Immunology 2024Quote: ... 1 µg/mL anti-murine IL-2 (Cat# 14-7022 Invitrogen Carlsbad, CA), 20 ng/mL murine IL-6 (Cat# 216-16 Peprotech Cranbury ...
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bioRxiv - Neuroscience 2024Quote: ... IL-6 serum levels were measured using an ELISA kit (88-7064, Invitrogen). The assay was performed following the manufactureŕs instructions and guidelines ...
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bioRxiv - Immunology 2024Quote: ... and IL-17A BV711 (#407-7177-82, Thermo Fisher Scientific, Houston, TX, USA). Cells were assessed using a 4-laser Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... The blots were washed and incubated with secondary antibodies (Thermo Scientific, IL, USA). The protein bands were detected by the standard chemiluminescence method (Thermo Pierce Femto ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Cell Biology 2021Quote: HEK293T (ATCC® CRL-3216™) and S1/S2 DKO HEK293 cells were maintained in cell culture in 1X DMEM (41965-039, Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An inducible D540K hERG HEK293 cell line (T-REx D540K hERG HEK) was generated using the Flp-In T-REx system (Thermo Fisher Scientific, MA, USA). The D540K hERG mutant was made site-directed mutagenesis from the wild-type (WT ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321; Thermo Fisher Scientific, Waltham, MA) at a density of ∼0.5-2 x105 cells per well and grown to ∼80-90% confluence with standard media ...
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bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant single-strand AAV vectors were produced using the ultracentrifugation method described in a previous paper were produced using HEK293-T cells (HCL4517; Thermo Fisher Scientific; Waltham, MA), as described previously26 ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...