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Citations for Thermo Fisher :
3401 - 3450 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human EGF (Life Technologies), 20ng/mL recombinant human FGF-basic (Fisher Scientific)] ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary human hepatocytes (Gibco/Thermo Fisher Scientific) were thawed into a 24-well Collagen I coated plate (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary human hepatocytes (Gibco/Thermo Fisher Scientific) were thawed into a 24-well Collagen I coated plate (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and 5 mg/mL human insulin (ThermoFisher) freshly supplemented with 100 ng/mL IL-34 ...
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bioRxiv - Immunology 2024Quote: ... biotin anti-human IgA Fab (Invitrogen A24462) was added at a dilution of 1:100,000 for 2 hours at 4 C to detect human IgA ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Molecular Biology 2024Quote: Signaling via the intracellular Ca++ pathway was assessed in stably transfected HEK- HEK293/hPTH1R/glosensor (GP-2.3) cells using the calcium-sensitive fluorophore Fura2-AM (Invitrogen, Life Tech. Grand Island, NY, USA) [73 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 hours to induce trapping of PARP1 as previously described.13 Cellular fractionation was performed with the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific, 78840) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Final protein concentrations were adjusted to 13 μg/μl in lysis buffer and NuPAGE 4x LDS sample buffer (NP0007, Thermo Fisher Scientific) was added to a final concentration of 1x and lysates were denatured at 95°C for 5 min ...
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bioRxiv - Genetics 2021Quote: ... Differentiation efficiency was quantified using flow cytometry as described previously 59 using mouse monoclonal antibody against cardiac troponin-T (clone 13-11, Thermo Fisher Scientific). All iPS-CM cultures used in this study were >85% cTnT+ ...
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bioRxiv - Immunology 2019Quote: ... The tissues were then incubated overnight with various combinations of a ZIKV in-house antibody [13] (1:100) and CD 163 (Thermofisher; 1:50) at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 13 µL of each sample was electrophoresed on a 4%–12% Bis-Tris protein gel (NuPAGE Novex, (ThermoFisher Scientific, Waltham, MA, USA) for 35 minutes at 200 V using MOPS buffer in a PowerPac™ HC Power Supply (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... 30nM of the respective siRNAs were reverse transfected into 10,000 (for dose curves and proliferation assays) or in 4×105 SUV420H2 mutant clones using Lipofectamine RNAiMAX (13-778-150, Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were centrifuged for 5 min at 13 000 × g at 4°C and the concentration of protein was measured using a bicinchoninic acid assay (Thermo Fisher Scientific). Protein lysates were fractionated by SDS-PAGE and transferred onto PVDF membranes ...
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bioRxiv - Neuroscience 2020Quote: ... the labium was removed by forceps and immediately flash-frozen in DNA Lo-bind nuclease-free tubes (Fisher Scientific #13-698-790) contained in a CoolRack (Biocision #BCS0137 ...
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bioRxiv - Microbiology 2020Quote: Rabbit kidney cells (RK-13, ATCC, CCL37) and 293T cells (ATCC, CRL-3216) were routinely maintained in minimal essential medium (MEM) (Life Technologies, USA) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells seeded on 13 mm glass coverslips (Agar Scientific) were incubated with MitoTracker Red (mitochondrial marker, Invitrogen, 1 μM in DMEM) for 30 min at 37°C or co-transfected with PHPLCd-RFP (plasma membrane marker ...
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bioRxiv - Molecular Biology 2020Quote: RNA was reverse transcribed and amplified using a switching mechanism at the 5’ end of the RNA template 13 reaction with Maxima reverse transcriptase (Thermo Fisher Scientific), and oligos listed in Table S1 ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots of 13 μl were loaded on 3% agarose gels (Invitrogen UltraPure Agarose 1000, 1X TAE buffer, Invitrogen SYBR Safe Gel Stain) and electrophoresed for 30 minutes at 100 volts ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells were cultured on 13 mm round coverslips in 35 mm dishes and transfected with pAAV/sCSF1R using Lipofectamine 2000 (Thermo Fisher Scientific). One day after transfection ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: ... Ter119-positive differentiated cells were recognized using specific biotin-conjugated antibodies (13-5921-81) and removed from the cell suspension using Streptavidin-coupled magnetic beads (65001; both reagents from Thermo Fisher Scientific). To induce erythroid differentiation ...
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bioRxiv - Biophysics 2021Quote: ... The nucleic acid-specific fluorochrome SYTO 13 green fluorescent nucleic acid stain in 5 mM solution in DMSO (Molecular Probes, Eugene, OR) was used in all experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit antibody to pSMAD 1/5/8 (1:100; CST 9516) and mouse antibody to beta-amyloid (1:100; Invitrogen 13-200) were diluted in the same 3% blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We removed the GS linker adjacent to the HiBiT tag from HiBiT-FZD7 [13] using GeneArt site-directed mutagenesis (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Molecular Biology 2022Quote: Eluted RNA samples from the in vitro transcription reactions were diluted based on concentrations measured on a Nanodrop spectrophotometer (13-400-519, Thermo Fisher Scientific) and denatured at 70°C for 2 minutes and snap-cooled on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with 250 pmoles of antisense oligonucleotide (ASO)22 and 10 uL of Lipofectamine RNAiMAX (Fisher Scientific, 13-778-030) per well according to manufacturer’s instructions for forward transfection ...
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bioRxiv - Cell Biology 2024Quote: ... after which the samples were centrifugated at 13 000 rpm for 15 min to remove residual debris and transferred into 250 µl autosampler microvials (Thermo Fisher Scientific). Samples were then loaded into an Easy-nLC 1200 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were washed and incubated with 30 μl mouse anti-total-Ub detection antibody (1:500, P4D1, Thermo Fisher, 13-6078-82) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were treated by 4% paraformaldehyde for a post-fixation and were stained by SYTO 13 (Cat. No. S7575, Thermo Fisher Scientific) for nuclei staining.
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bioRxiv - Immunology 2024Quote: ... for 13 hours at 37 °C and 5% CO2 in the presence of Brefeldin A (3 µg/mL; ThermoFisher; Waltham, MA, USA) for the last 10 hours of stimulation ...
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bioRxiv - Molecular Biology 2024Quote: ... over 60 min and 28% to 45% B over 13 min at 350 nl/min delivered by an Easy1200 nLC system (Thermo Fisher Scientific) with a total 90 min method length.
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bioRxiv - Immunology 2022Quote: ChIP-Seq experiments were performed as previously described (Li et al., 2017; Lin et al., 2012) using the following antibodies: anti-STAT5B (Invitrogen 13-5300), anti-H3K27Ac (Diagenode) ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes and microglia/macrophage cells were labeled using rat anti-GFAP (1:300; Cat# 13-0300, Thermo Fisher Scientific, Waltham, MA, U.S.A) and rabbit anti-IBA1 (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were blotted at 100% humidity for 3 s (13°C, 0 s drain time, blot force -3 to +2) with a Vitrobot Mark IV (Thermo Fisher Scientific) and plunged into liquid ethane ...
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bioRxiv - Cancer Biology 2023Quote: ... In total, 13 fractions were collected for each batch, dried in a vacuum concentrator (SpeedVac SC110 Savant, (Thermo Fisher Scientific, Bremen, Germany)) ...
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bioRxiv - Immunology 2023Quote: ... The debris was washed with PBS (5 min, 13 000 x g, RT) and labeled for 1 hour with pHrodo Red succinimidyl ester (Thermo Fisher Scientific) with 2 μL of a 10 mM stock per 10×106 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA of all samples was then amplified using 13 PCR cycles (Veriti 96-Well Thermal Cycler, Applied Biosystems, Thermo Fisher Scientific). After a cleanup (SPRIselect ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA of all samples was then amplified using 13 PCR cycles (Veriti 96-Well Thermal Cycler, Applied Biosystems, Thermo Fisher Scientific). After a cleanup (SPRIselect ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were labeled with 1 μg/mL mAb 13-2 (rOmpB) (53) and 1 μg/mL rabbit polyclonal αTGN46 (Invitrogen, PA5-23068) in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were sliced into 20-μm sections using a cryostat (temperatures: sample holder −13°C, blade −12°C) (ThermoFisher CryoStar™ NX70) and mounted on superfrost slides (Fisher #1255015) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR assays were performed using TaqMan Gene Expression Master Mix and the probes human NEMO/IKBKG (Hs00415849_m1) and human GAPDH (Hs03929097_g1) (ThermoFisher Scientific). Amplifications were run in a Bio-Rad CFX96 real-time PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... The second incubation were performed with secondary antibody (Anti-Human IgG-Cy3, Jackson ImmunoReserarch; Anti-Human IgM-Cy5, Invitrogen) diluted in ratio 1:1000 in incubation buffer with 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: Human monocytic U937 cells (ATCC) and human embryonic kidney 293T cells (ATCC) were cultured in RPMI 1640 medium (Gibco), supplemented with 5-10% fetal calf serum (BioWest) ...
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bioRxiv - Cancer Biology 2021Quote: Human HCC cell lines (Huh7, HepG2) and Human HeLa were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco-BRL) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... Human AFP secreted into the sera of animals were determined by the Human AFP Elisa Quantification Kit (Invitrogen, EHAFP) following manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... In the analysis of the Woroniecka Human Diabetic Kidney Disease dataset (2011, Affymetrix Human Genome U133A 2.0 Array platform) 33 and the European renal cDNA bank (ERCB ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...