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Citations for Thermo Fisher :
3101 - 3150 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 000 cells were seeded onto a 6-well plate and reversely transfected with 25 nM of either non-targeting control siRNA (Horizon Discovery, D-001206-13-20) or SMARTpool siRNA targeting Znf706 (Horizon Discovery, M-021025-01-0005) using Lipofectamine RNAiMAX (Invitrogen, 13778150). The transfection media was exchanged after 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Cultures were stimulated with phorbol 12-myristate 13-acetate (PMA) (5 ng/mL, Alfa-Aesar) + Ionomycin (0.5 µg/mL, Thermo Fisher Scientific) and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL ...
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bioRxiv - Developmental Biology 2023Quote: Dechorionated Cab and Kaga embryos at the 12-13 somite stage were placed in Gibco CO2 independent medium (Thermo Fisher #18045054). Using a forceps and a scalpel ...
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bioRxiv - Pathology 2023Quote: ... Rhabdomyolysis-AKI (rhabdo-AKI) was induced in male mice aged 13-14wk were given intramuscular (IM) 50% glycerol (Invitrogen, Carlsbad, CA) in sterile water after water deprivation for 18hrs prior to injection ...
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bioRxiv - Cell Biology 2022Quote: ... The flow through fraction was then fractionated into 13 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher).
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bioRxiv - Biochemistry 2023Quote: ... then incubated overnight at 4C in 500 μL of primary antibody (1 μg/mL Ms anti myc, Invitrogen cat. #13-500) diluted in blocking / permeabilization buffer (PBS + 4% FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... A standard amount of protein (13-20ng) was denatured in a total volume of 40µl with LDS buffer and Reducing Agent (ThermoFisher Scientific, B0009) at 70°C for 10 min ...
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bioRxiv - Systems Biology 2023Quote: We conducted mass spectrometry analysis of 120 CSF samples using 13 batches of 11-plex tandem mass tag (TMT, Thermo Scientific) experiments ...
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bioRxiv - Cell Biology 2024Quote: Three hundred thousand INS1(832/13) or EndoC-βH3 cells were cultured in 12-well plates (Cat#150200, Nunc, Buckingham, England), and pre-incubated for two days ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected at about 70% confluency with either siGENOME non-targeting control siRNA (Dharmacon, D-001206-13-05) or indicated siGENOME SMARTpool siRNA (Dharmacon) using Lipofectamine RNAiMAX (ThermoFisher, 13778100) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Mechanically dissociated cerebral organoids were filtered through a 35 µm strainer into a low protein binding tube (cat#: 13-864-407; Fisher Scientific). Cell solution was centrifuged at 300g ...
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bioRxiv - Molecular Biology 2024Quote: ... patient-derived immortalized myoblasts and non-DUX4 expressing cells (54-6; 13 D4Z4 repeat units) were grown in Ham’s F-10 Nutrient Mix (Gibco, Waltham, MA) with 20% FBS (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 20-30 minutes in SuperBlock solution (ThermoFisher, Rockland, IL) at room temperature before overnight incubation at 4°C in 3% skim/tris buffered saline (TBS ...
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bioRxiv - Immunology 2021Quote: ... Anti-IL-2 Ab conjugated to PE (clone JES6-5H4) manufactured by Invitrogen, purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentration was determined using BCA protein assay kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using the BCA assay (Thermo Scientific, Rockford, IL, USA). Mitochondrial respiration of isolated mitochondria was measured within two hours of isolation.
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bioRxiv - Immunology 2022Quote: ... RNA purity and quantity were evaluated with Nanodrop spectrophotometer (Thermo Scientific, Rockford, IL). cDNA synthesis was performed using EvoScript Universal cDNA Master ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with a protease and phosphatase inhibitor cocktail (Thermo Scientific, Rockford, IL, USA) using a Potter-homogenizator ...
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bioRxiv - Immunology 2021Quote: ... and IL-12p70 production were measured using the cytokine kits purchased from Invitrogen and following the instructions from the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... and murine IL-6 were measured using commercial ELISA kits obtained from Invitrogen, Thermo Fisher Scientific (Lower detection limits ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM NaF) containing Halt protease cocktail inhibitor (Thermo Scientific, Rockford, IL, USA). Following ten minutes incubation on ice ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were determined by BCA protein assay (Thermo-Fisher Scientific, Rockford, IL). Protein samples were reduced with 10 mM TCEP and alkylated with 25 mM iodoacetamide before trypsin digestion in 1/40 enzyme/protein ratio at 37°C overnight.
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bioRxiv - Neuroscience 2020Quote: ... Bicinchoninic Acid (BCA) protein assay kit was obtained from Thermo Scientific (Rockford, IL). ProSignal Blotting Film was purchased from Genesee Scientific (El Cajon ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Cancer Biology 2021Quote: ... The same medium with addition of 10ng/ml IL-6 (Gibco, Ref: PHC0066) was used for primary MM plasma cell cultures ...
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bioRxiv - Cell Biology 2021Quote: ... Pierce™ Anti-HA Agarose (26181) were purchased from Thermo Scientific (Rockford, IL). Deubiquitinases (DUBs ...
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bioRxiv - Microbiology 2021Quote: Quantification of IL-1β secretion in cell supernatant was measured by ELISA (Invitrogen, IL-1 beta Mouse Uncoated ELISA Kit ...
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bioRxiv - Microbiology 2021Quote: ... Tryptic peptides were further desalted using C18 spin columns (Thermo Fisher Scientific, IL). Samples were then lyophilized and reconstituted in 0.1% trifluoroacetic acid to be analyzed in quadruplicates without fractionation for quantitation purposes.
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific, IL, USA) and a total of 100 µg of protein per sample was subjected for trypsin digestion ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized by chemiluminescence (Pierce Supersignal West Dura, Thermo Scientific, IL, USA) and quantified with ChemiDocTM MP imaging system (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: ... and purified using the MEGAclear Transcription Clean-Up kit (Thermo Fisher, Chicago, IL) as described by Vilarino et al10 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with protease and phosphatase inhibitor cocktail (PPI; Thermo Scientific, Rockford, IL, USA) and EDTA (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc, Rockford, IL, USA) in PBS and immediately imaged using a Zeiss Imager (larval FB ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ and IL-17A were measured with uncoated ELISA kits from Invitrogen in accordance with manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... Proteins were denatured using NuPAGE LDS Sample Buffer (Thermo Fisher Scientific, Rockford, IL). The proteins were resolved by SDS-PAGE on 4-12% NuPAGE gels using NuPAGE MOPS buffer (NuPage gels ...
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bioRxiv - Cell Biology 2021Quote: ... The blots were visualized using SuperSignal-enhanced chemiluminescence (Thermo Scientific, Rockfold, IL, USA) and chemiluminescence function on a GE Healthcare Amersham AI 600 RGB imager ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of IL-2 standard (Invitrogen eBioscience, catalog no. 14-8021-64) or supernatants are added to the wells ...
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bioRxiv - Physiology 2021Quote: ... pH – 7.5) with phosphatase and protease inhibitors (HALT Thermo Scientific, Rockford, IL, USA) using a tissue homogenizer (Bullet Blender ...
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bioRxiv - Immunology 2020Quote: ... IgM (Il/41-PE-Cy7, eBioscience) and live/dead Near-IR (Life Technologies). A secondary staining step was performed using streptavidin-BUV395 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit anti-SARS-CoV nucleocapsid polyclonal antibody (PA1-41098, Thermo Scientific, Rockford, IL) diluted 1:2,000 in blocking buffer was allowed to bind overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and counterstained with Richard-Allan Scientific Modified Mayer’s Hematoxylin (Thermo Scientific, Rockford, IL) and 0.2M lithium carbonate (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Maturation was induced by a combination of IL-1β (10 ng/ml; Invitrogen), IL-6 (100 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... or IL-2 Mouse Uncoated ELISA Kit (Thermo Fisher Scientific, 88-7024-76) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and IL-10 were analyzed by ELISA as described by the manufacturer (ThermoFisher).
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bioRxiv - Cell Biology 2019Quote: ... and were imaged in FluoroBrite™ DMEM media (Thermo Fisher Scientific, Rockford, IL) containing 5% FBS and 20 mM HEPES at 37°C ...
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bioRxiv - Immunology 2019Quote: ... emulsified in 2 mg alum (Imject™ Alum, Thermo Fisher Scientific, IL, USA). Non-allergic control animals (PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-mouse CLDN5 (Cat #34-1600, Thermo Fisher Scientific, Rockford, IL, USA), rabbit anti-mouse LYVE-1 (Cat # 11-034 ...
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bioRxiv - Microbiology 2019Quote: ... Protein was quantitated by bicinchoninic acid assay (Thermo Scientific Pierce, Rockford, IL, USA) and analyzed with SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... and collected in Superfrost® Plus slides (Thermo Fisher Scientific, Rockford, IL, USA). For laser microdissections ...
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bioRxiv - Microbiology 2020Quote: ... and anti-IL-2 (No. 25-7021; eBioscience/Affymetrix, Santa Clara, CA, USA) antibodies at room temperature for 30 min ...