Labshake search
Citations for Thermo Fisher :
3051 - 3100 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... followed by absorbance measurements at 450nm using a Multiskan FC Microplate Photometer (51119000; Thermo Scientific). As a positive control ...
-
bioRxiv - Plant Biology 2024Quote: ... and reading absorbance at 620 nm in a photometer (Multiskan FC, Thermo Scientific, United States) and using a glucose standard curve to compare the colorimetric response of the samples ...
-
bioRxiv - Immunology 2024Quote: ... containing 10% FCS (Biochrome, Cat. S0615-500ML) and 1% Penicillin/ Streptomycin (Thermo Fisher, Cat. 7001592). After sorting ...
-
bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies with HRP (ab97250 ...
-
bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
-
bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
-
bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
-
bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
-
bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
-
bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
-
bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
-
bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
-
bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
-
bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
-
bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
-
bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
-
bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... 9101) were purchased from Cell Signalling. Mouse monoclonal anti tot-ERK1/2 (Cat. Nr. 13-6200) was purchased from Thermo Fisher. Mouse monoclonal anti-α-Tubulin (Cat ...
-
bioRxiv - Developmental Biology 2020Quote: ... Embryos at the early segmentation stage (13 hpf) were treated similarly but incubated with 500 ul of 0.25 Trypsin (Gibco, Cat#15090046)/ EDTA instead of TrpLE (Gibco ...
-
bioRxiv - Biochemistry 2019Quote: ... The valve then switches to engage the passive split such that a majority of LC effluent is diverted to waste prior to the third dimension PC. 13 RP-SAX-RP fractions were analyzed. Either an LTQ Velos (Figs. 1, 7) or Q-Exactive HF (Thermo Scientific) machine was used to acquire MS/MS spectra in data-dependent mode as described (Zhang et al. ...
-
bioRxiv - Genomics 2020Quote: ... the membranes were washed three times in 13 ml PBS and the bound antibodies were detected using the SuperSignal West Femto maximum sensitivity substrate (Thermo Scientific) according to manufacturer’s protocol and the LI-COR Odyssey system (LI-COR Biosciences).
-
bioRxiv - Neuroscience 2021Quote: ... All cell populations were isolated in a single sort using a Becton Dickinson FACS Aria II using 13 psi pressure and 100-µm nozzle aperture and were collected in Neurosphere medium (NSM) [DMEM/F12 (Life Technologies) supplemented with 0.6% Glucose (Sigma) ...
-
bioRxiv - Neuroscience 2021Quote: ... and imaged or fixed on 13-15 DIV. Neurons were transfected as described in (Sheehan et al. 2016) using Lipofectamine 2000 (ThermoFisher/Invitrogen) on 9 DIV ...
-
bioRxiv - Biophysics 2021Quote: ... was applied to glow-discharged 300 mesh gold grids (CryoMatrix M024-Au300-R12/13) and vitrified using a FEI Vitrobot Mark IV (ThermoFisher Scientific), at 4 °C and 100% humidity with blot time of 1 s and blot force of 0 followed by flash-frozen in liquid ethane ...
-
bioRxiv - Cell Biology 2021Quote: ... Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific) following addition of 4 mg/mL polybrene (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
-
bioRxiv - Neuroscience 2022Quote: ... 40 μl of streptavidin magnetic beads were incubated with 4 μg of anti-CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, #13-1719-82, Thermo Fisher) for three hours on ice ...
-
bioRxiv - Microbiology 2022Quote: ... 1 ml aliquots of CSF from sixty-three PIH patients [13] were inoculated into BD BACTECTM Lytic Anaerobic Medium culture bottles supplemented with 1 ml of defibrinated horse blood (Thermo Scientific). Culture bottles were incubated in a BD BACTECTM FX instrument and monitored for bacterial growth for up to 14 days ...
-
bioRxiv - Genetics 2022Quote: ... Reverse transcription of 13 µl of total isolated RNA was achieved using the High Capacity RNA-to-cDNA kit (Applied Biosystems) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells (INS-1 832/13 and HEK-293T) were seeded onto poly-D-lysine coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a cell density of 70,000 cells/cm2 ...
-
bioRxiv - Immunology 2020Quote: ... cell materials derived from SSChigh group (day 13) which was enriched in large numbers of terminally differentiated CD8+ T cells were labelled with CellTrace Far Red (Invitrogen, C34564) following the manufacturer protocol ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... were purchased from micromod Partikeltechnologie GmbH (Germany) and characterized by dynamic light scattering (DLS) and zeta potential measurements as described previously.13 Heat-inactivated FBS (ThermoFisher Scientific) was centrifuged at 16,000g for 3 min to remove any insoluble aggregates and the supernatant was mixed with nanoparticles at final concentrations of 90% (v/v ...
-
bioRxiv - Genetics 2021Quote: ... followed by microglial differentiation media for 13 days (consisted of: DMEM/F12 HEPES no phenol red, 2% ITS-G (Life Technologies), 1% N2 supplement (Life Technologies) ...
-
bioRxiv - Molecular Biology 2021Quote: ... the membranes were probed with the following primary antibodies overnight at 4°C with gentle shaking: anti-p53 (13-4100; 1:500) and anti-p21WAF1 (AHZ0422; 1:250) antibodies were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: Biofilms were developed on plastic coverslips (diameter = 13 mm, thickness = 0.2 mm) (Nunc Thermanox TM, Thermo Fisher Scientific, Waltham, MA, USA) in the presence or absence of sub-MICs of TC at 37°C for 24 h ...
-
bioRxiv - Cell Biology 2022Quote: ... Huh7 YFP-TIA1 Neo PKR Blr cells and Huh7 PKR Blr cells (PKROE)(13) were additionally supplemented with 5 μg/ml blasticidine (Life Technologies). Huh7 GADD34 Puro cells (13 ...
-
bioRxiv - Neuroscience 2021Quote: ... 13) anti-Lectin antibody (#DL-1174, Vector, Burlingame, CA, USA) and anti-CD144 antibody (#14-1441-82 Invitrogen, Carlsbad, CA, USA) to label vascular and endothelia.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Murine myoblasts (C2C12) were purchased from Public Health England at Passage 13 and seeded into either T-75 flasks (Biolite, Thermofisher Scientific), 6 well plates (Biolite ...
-
bioRxiv - Microbiology 2022Quote: Cell suspensions containing formaldehyde-fixed AnTat1.1E 90–13 GFP::PAD1utr parasites were stained with a PBS solution containing 0.005 mg/mL Hoechst 33342 (Thermo Fisher Scientific) for 20 min at 4°C.
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... THP1 cells were differentiated into macrophage-like cells by treatment with 10nM phorbol 12-myristate 13-acetate (PMA) for a period of 72h. Lipofectamine2000 (cat no. #11668019) from Thermo Fisher Scientific or Helix-IN reagent (cat no ...
-
bioRxiv - Immunology 2022Quote: Cells were seeded in a 24-well plate on sterile 13 mm coverslips and fixed for 10 minutes with cold 4% w/v formaldehyde (Fisher Scientific) and cold 8% w/v formaldehyde in HEPES buffer ...
-
bioRxiv - Neuroscience 2024Quote: WT and PINK1 KO HEK293E cells were previously described [13] and were cultured in Dulbecco’s modified Eagle medium (Thermo Fisher, 11965118) supplemented with 10% fetal bovine serum (Neuromics ...
-
bioRxiv - Cell Biology 2023Quote: ... medium containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific) following addition of 4 mg/ml polybrene (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C for 18 hours with primary antibodies: E-Cad (13-1700, Thermo Fisher, HECD-1, 1:100) and CK-14 (RB-9020 ...
-
bioRxiv - Cell Biology 2024Quote: ... day 10 and day 13 cells from the Laminin EHT culture were detached from the adherent layer using Accutase (Life Technologies’) at 37°C for 5’ ...
-
bioRxiv - Immunology 2024Quote: ... non-sun-exposed skin as previously described(13) and used at passages 2-6 and grown in Epilife medium (Gibco, #MEPI500CA) with added human keratinocyte growth supplement (10ul/ml medium) ...