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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The cells were passaged every 3-4 days after they achieved confluency of 80-90% using Trypsin 0.25% EDTA (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... the cell cultures were washed 3 times with 37 °C DPBS+ and fixed using 4% w/v paraformaldehyde (PFA; Affymetrix) in PBS for 2.5 h ...
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bioRxiv - Genetics 2022Quote: ... LC-MS/MS based proteomics datasets (4 groups × 3 replicates) were then generated in a lab (code: NVG) using Q Exactive HF-X mass spectrometer (ThermoFisher). To eliminate confounding factors such as experimental sample-processing order with sample group ...
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bioRxiv - Neuroscience 2022Quote: ... slices for optical recordings were incubated for 30 minutes in oxygenated Krebs solution added to a 3% Di-4-ANEPPS (Invitrogen) stock solution and mixed with an equal volume of fetal bovine serum (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... mRNA samples of 4 ng were subjected to DNaseI treatment and 3’ dephosphorylation using FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) and T4 PNK (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... then equal quantities of protein lysate (3-10 µg) were separated by SDS-PAGE using 4–12% gradient gels (Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Genomics 2024Quote: ... and transfer plasmids were transfected at a mass ratio of 2:3:4 with Lipofectamine 3000 (Thermo Fisher Scientific L3000001) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell bodies were removed by centrifugation (1,150 g, 3 min, 4°C; Sorvall Legend XTR, Thermo Fisher Scientific). A sucrose cushion (10 ML of 25% sucrose in HMS ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2023Quote: ... For differential glucose concentration treatments between day 2 and 3 and between day 3 and 4, N2B27 was made using DMEM/F12 without glucose (L0091-500, Biowest) and Neurobasal without glucose (A2477501, ThermoFisher). For 1X and 3X glucose concentrations ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Neuroscience 2023Quote: ... Media was partially renewed every 3-4 days with neuronal differentiation media 2 (NDM2: 1:1 DMEM/F12:NB (Gibco), glutamax (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: ... islets from 3-4-week-old control and β-LonP1KO mice were handpicked and then dispersed with trypsin (Thermo Scientific). Islet cells were counted and incubated with adenovirus expressing an empty vector control (Ad-RIP2 EV ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 nM LysoTracker Red DND-99 (Thermo Fisher Scientific)) ...
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bioRxiv - Systems Biology 2023Quote: ... 4 μL 5× phusion HF buffer (Thermo Scientific), 4 μL 5 M Betaine (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug dilution series (1:3) were prepared in PDAC organoid culture medium containing 1 μg/ml Hoechst (Invitrogen) and 1 μg/ml PI (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... CVL was performed by washing the cervicovaginal cavity with 3 mL of phosphate buffered saline (PBS, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Bioengineering 2020Quote: ... the collagen solution was prepared by mixing 50% (v/v) of 3 mg/mL type 1 collagen (ThermoFisher) with 2.5% of 1N NaOH ...
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bioRxiv - Neuroscience 2019Quote: ... 0.5 μl of DiI (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate, 2.5 mg/mL in dimethyl sulfoxide, Thermo Fisher Scientific) were injected into the stratum moleculare of the dentate gyrus of the hippocampal filed using a motorized microinjector (Stoelting ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured neurons were incubated with 3 μg/mL of the ratiometric calcium indicator Fura-2 AM (Life Technologies) in external recording solution for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEPs were cultured with 10 μg/mL 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindo-carbocyaninelabeled Ac-LDL (Thermo Fisher Scientific) at 37 °C for 5 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... two small pieces (~ 10 mg) were placed in 3 mL of oxygenated DMEM CO2 independent media (ThermoFisher #18045088) at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2 mg/ml proteinase-K (Invitrogen; 25530049) for 10 min at 55 °C ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 μg plasmid and 1.5 μl Fugene HD (m/v=1:3) were diluted and incubated in 0.25 ml OptiMEM (Gibco). After 5 min ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was isolated using the Invitrogen GeneCatcher gDNA 3–10 ml Blood Kit (Life Technologies, Carlsbad, CA) or Gentra PureGene (Qiagen ...
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bioRxiv - Immunology 2021Quote: 1 × 106 TBK1.1 spores were inoculated into a flask containing 3 ml RPMI 1640 medium with HEPES (Gibco) containing 2% glucose in a 37°C shaker at 100 rpm in triplicate ...
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bioRxiv - Immunology 2021Quote: B cells (3×106) were pelleted by centrifugation and resuspended in 0.3 ml of Trizol (Thermo Fisher Scientific) and RNA spike-in (generated by in vitro transcription ...
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bioRxiv - Genomics 2021Quote: ... Fragment mRNA was then incubated with 0.5 μl of Random primers (3 mg/mL) (Thermo Fisher Scientific 48190011), 0.5 μl of 50mM Oligo dT primer ...
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bioRxiv - Immunology 2022Quote: ... Primary B cells were plated at 2-3×106 cells/ml in primary B cell medium (DMEM (Gibco) containing 10% FBS (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were collected and washed with 1 x HBSS containing 1% fetal bovine serum then suspended in 1.0 ml of 1 x HBSS containing 2.5μg/ml of Fluo-3-AM dye and 2.5μm of the anion carrier inhibitor probenecid (Thermo Fisher Scientific) and incubated for 45 min at room temperature on an orbital shaker in the dark ...
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bioRxiv - Microbiology 2024Quote: ... we followed the vendor supplied protocol of the His-Pur Ni-NTA Purification Kit (3 ml, Thermo Fisher). The flow through ...
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bioRxiv - Bioengineering 2024Quote: A stock solution of collagen type I (collagen I, rat tail Gibco, 3 mg/mL – cat #A10483-01) was prepared on ice with 10X PBS ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using 0.5 - 3 mL 3.5 MWCO Slide-A-Lyzer™ Dialysis Cassette (Thermo Fisher Scientific) at 4°C for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and were coated with 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate stain (DiI stain, 2.5 mg/ml, Thermo Fisher, D282) diluted in one part of ethanol and 9 parts of water ...
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bioRxiv - Microbiology 2023Quote: ... of confluent MDCK cell monolayers overlaid with 3 ml of Leibovitz’s L-15 medium with L-glutamine (Gibco), 0.2 mM HEPES buffer ...
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bioRxiv - Bioengineering 2022Quote: Cultured neurons were washed 3 times with 1 mL of pre-warmed serum free Neurobasal medium (Gibco, 21103049) supplemented with 4% B-27 (Gibco ...