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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...
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bioRxiv - Biochemistry 2023Quote: ... BODIPY-CE (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3- dodecanoate) was obtained from Life Technologies. Lipids (with the exception of NBD-LPC ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 3-4 days during the log phase of growth with Trypsin/EDTA solution (Gibco, R001100) following a PBS wash ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse cochleae were fixed in 4% PFA for 3 hours and microdissected in cold 20% RNAlater (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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bioRxiv - Neuroscience 2022Quote: ... each well-being finally filled with 3 mL of neurobasal phenol red-free medium (ref. 12348017, ThermoFisher) containing 0.5 mM GlutaMax (ref ...
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bioRxiv - Neuroscience 2021Quote: ... 3 % of homogenate was added to 1 mL of TRIzol Reagent (Fisher Scientific cat# 15-596-026) and RNA was isolated following the user guide referenced above ...
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bioRxiv - Genomics 2020Quote: ... TALE expressing ESCs were cultured in the presence of 3 μg/mL blasticidin (Invitrogen Cat# R210-01) and ESC lines stably expressing 3xHA-tagged open reading frames were cultured in the presence of 1 μg/mL puromycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... the growth medium was removed by aspiration and the cells were washed with 3 mL PBS (Gibco), before adding 3 mL of Cell Stripper (Corning) ...
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bioRxiv - Biophysics 2022Quote: ... The tether was then moved to Channel 3 containing 0.5 mg/mL salmon sperm DNA (Thermo Fisher) in HR buffer except for MCM/ORC salt stability assays ...
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bioRxiv - Bioengineering 2022Quote: ... Permethylated samples were combined in 1:3 ratio with 10 mg/mL 3,4-diaminobenzophenone (DABP, Acros Organics) matrix in 75% acetonitrile ...
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bioRxiv - Neuroscience 2022Quote: ... was repeatedly submerged in 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI) lipophilic dye (2.5 mg/ml, in 70 % ethanol, Thermo Fisher) and then slowly inserted into the cortex at an angle of 20 ° from the vertical (with respect to bregma ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... Wells were washed 3 times and incubated with 1 μg/ml biotinylated anti-human IgE (A18803, Invitrogen) in 1% skim milk for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... and layered onto a 3 ml bed of growth media with 0.5% low-melt agarose (Fisher Scientific). Cells were fed daily with 1 ml of growth media ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed 3 times with PBS and an aliquot of 1 ml of warmed TrypLE (Gibco) added to each well and incubated at 37°C for 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: Zebrafish larvae were anesthetized in 0.14 mg/ml ethyl 3-aminobenzoate methanesulfonic acid salt (#118000500, Acros Organics) and examined daily under a Zeiss Axio Zoom V16 fluorescent microscope for leakage of DBP-EGFP from the hyaloid vessels from 3 dpf – 7 dpf.
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bioRxiv - Immunology 2021Quote: ... with confluent bEnd.3 cell monolayers or pre-coated with collagen I (1 mg/ml, Life Technologies), and allowed to adhere for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... then 3 mL room-temperature 1X Dulbecco’s phosphate-buffered saline (DPBS) (14040141; Thermo Fisher Scientific, Waltham, MA) was added onto each well ...
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bioRxiv - Neuroscience 2022Quote: ... 3 % of homogenate was added to 1 ml of TRIzol Reagent (Fisher Scientific cat# 15-596-026) and RNA was isolated as per the user guide referenced above ...
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bioRxiv - Genetics 2024Quote: ... before being dissociated from the culture flask (T75 cm2) with 3 mL 0.05% trypsin/EDTA (Gibco™) for 5-10 minutes at 37°C depending on the cell line ...
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bioRxiv - Molecular Biology 2024Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... supernatant was overlaid onto a 3 ml 60% iodixanol cushion and centrifuged (Thermo Scientific Sorvall WX Ultra) at 100000 g (SW28Ti rotor ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then loaded onto 3 mL of pre-equilibrated Ni-NTA resin (Thermo Fisher Scientific). The column was washed with 30 mL of lysis buffer followed by 90 mL of wash buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... Single cells were prepared in 3 ml enzyme-free dissociation buffer (Gibco, Thermo Fisher Scientific, Waltham, MA) incubated in a 37 °C water bath for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Samples were individually mixed with an equal volume of 1 µg/ml of 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI; Invitrogen), incubated for 15 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2mg/ml proteinase-K (Invitrogen; 25530049) for 10 minutes at 55°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Single cells were prepared in 3 ml enzyme-free dissociation buffer (Gibco, Thermo Fisher Scientific, Waltham, MA) incubated in a 37 °C water bath for 10 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 3 mL of the clarified lysate were incubated with 1800 U of RNase I (Ambion cat# AM2294) for 1 h at room temperature with gentle rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... Single colonies were picked up and inoculated in 3 mL LB Broth with Miller’s modification (ThermoFisher Scientific) at 37 °C shaking at 250 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... and fixed with 0.5 mL methanol-free paraformaldehyde 4% (Thermo Scientific™ Pierce™) for 30 min at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... All cell lines were cultured in a humidified 5% CO2 incubator at 37 °C with media supplemented with 100 U/ml penicillin and 100 µg/ml streptomycin (Life Technologies). K562 was cultured in RPMI-1640 (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 x 107, or 5 x 107 PFU/mL (MOI of 5, 50, or 100 respectively) in CO2-independent medium (Gibco Life Technologies) supplemented with 0.1% (w/v ...
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bioRxiv - Genomics 2024Quote: ... 2 ug/mL (for K562 cells) and 20 ug/mL (for iPSCs) puromycin (GIBCO/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% sodium pyruvate solution 100 mM (Thermofisher). Undifferentiated cells were cultured at 33 °C in the presence of 10U/mL murine IFN-gamma (R&D systems) ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were loaded onto a trap column (Acclaim PepMap 100 C18, 75 μm × 20 mm, 3 μm particle, 100 Å pore size, Thermo Fisher Scientific) at a flow rate of 5 μl/min and separated on an analytical column (Acclaim PepMap RSLC C18 ...
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bioRxiv - Cancer Biology 2019Quote: An aliquot of each final fraction was loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Cancer Biology 2020Quote: ... an aliquot of each enriched sample was injected onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, 3 μm, 100 Å, Thermo Scientific) coupled to an analytical column (EASY-Spray PepMap column ...
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bioRxiv - Molecular Biology 2020Quote: ... and loaded onto a reverse phase trap column (Acclaim PepMap 100, 75µm x 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, U.S.A.) using an Ultimate 3000 for 50 µL at a flow rate of 10 µL min-1 ...
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bioRxiv - Plant Biology 2021Quote: ... The desalted peptides were then separated using a 15 cm pre-packed reverse phase analytical column (Acclaim PepMap 100, 75μm x 15 cm, C18, 3 μm, 100 Å, ThermoFisher, Waltham, MA, U.S.A) using a 45 min linear gradient from 5% to 40% solvent C (80% acetonitrile ...
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bioRxiv - Plant Biology 2021Quote: ... The analytical column was an Acclaim™ PepMap™ 100 C18 column (75 μm × 150 mm, 100 Å, 3 μm, Thermo Scientific, USA). The mass spectrometer was operated in data-dependent acquisition mode ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were loaded onto an Acclaim Pepmap 100 C18 trap column (75 μm x 2 cm nanoViper, 3 μm, 100 Å) (Thermo Fisher Scientific) at 2 μL/min ...
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bioRxiv - Cell Biology 2023Quote: Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was loaded with 100 µL of a 5 µM Fluo-4 AM (Molecular Probes) solution in imaging buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... Extract was precleared by incubating overnight with 5 ug of mouse polyclonal IgG (Invitrogen, #31903) in the presence of 100 ug RNase A (Fisher scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and aqueous phase was precipitated with 1 volume of isopropanol and 5 ug Glycogen (Invitrogen) at -80 °C for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... 100 μL of 5 μg/mL fibrinogen from human plasma conjugated to AF488 (Invitrogen) was added to each well ...
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bioRxiv - Systems Biology 2022Quote: ... and the resulting supernatant (100 ng/ml) was incubated for 4 h at 37°C together with 5 mM glutamine (Gibco, Cat# 25030-081), 1 mM phosphoribosyl pyrophosphate (Sigma ...