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Citations for Thermo Fisher :
3051 - 3100 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and Taqman gene expression assays (human tyrosinase [TYR] Hs00165976_m1; mouse TH Mm00447557_m1; Slc6A3 [DAT] Mm00438388_m1; Slc18a2 [VMAT2] Mm00553058_m1, Applied Biosystems) using standard procedures in a 7900HT Fast Real Time Instrument (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated using the DynabeadsTM untouched human CD8 T-cell isolation kit (Invitrogen), stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™ ...
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bioRxiv - Developmental Biology 2024Quote: ... and adapter ligation were performed using an Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The cDNA library was purified using Agencourt AMPure XP Reagent (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... Human primary Müller cells (P3) at 80% confluency were transfected with MT1 (assay ID: s194623, ThermoFisher Scientific), AKAP12 (assay ID ...
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bioRxiv - Genetics 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in DMEM supplemented with 10% fetal calf serum (Gibco). Cells were seeded with 300 000 cells/well in 6-well plates and transfected 24 hours later with 1 µg of each minigene construct mixed with 3 µL of Lipofectamine 2000 Reagent (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Human skin: Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco) digestion for 20h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... falciparum blood-stage parasites were cultured in 2.5% hematocrit human RBC in malaria culture media (MCM) comprising of 10.43g RPMI 1640 powder (Gibco), 25ml 1M HEPES (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... The silencer select siRNA targeting human ERK3 and the Silencer non-targeting Control #1 were purchased from Ambion.
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cell Biology 2020Quote: Normal human fibroblast cell line (AG08469, Coriell Institute for Medical Research) was maintained in Minimum Essential Medium (Gibco) and supplemented with 15% FBS (HyClone) ...
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bioRxiv - Cell Biology 2020Quote: Silencer Select siRNAs against human SNX4 and VPS35, and nontargeting control “scrambled” siRNA (predesigned, 4,390,844) were purchased from Ambion® (Thermo Fisher Scientific) ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin complex was reconstituted by co-expression in human FreeStyle 293-F cells (Thermo Fisher Scientific). The GIP-2 and γ-tubulin (TBG-1 ...
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bioRxiv - Genomics 2021Quote: Human BLaER1 cells (14) were kindly provided by Thomas Graf (CRG, Barcelona) and grown in RPMI medium (Invitrogen) supplemented with 10% heat-inactivated foetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2021Quote: The human episomal line of induced pluripotent stem cells (A18945, Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was maintained in feeder-free culture on Geltrex matrix (A1413202 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human CD171 (i.e. antibody against neural cell adhesion molecule L1CAM; RRID: AB_2043813; Thermo Fisher Scientific, Waltham, MA) or anti-human tetraspanin CD81 (Ancell Corporation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and any antigen–MS-Hu6 complex was captured by goat anti–human HRP–conjugated IgG (Invitrogen, Catalog # A18805).
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bioRxiv - Microbiology 2022Quote: ... falciparum was cultured in O+ human RBCs (Interstate Blood Bank, TN) supplemented with RPMI1640 medium along with 0.3-0.5% Albumax I (Invitrogen), 2g/L sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: ... Human HAP1 cells were obtained from Horizon Discovery and were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco), containing L-glutamine and 25 nM HEPES and supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... 19-805) of human ACE2 (GenBank NM_021804.1) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA) ...
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bioRxiv - Molecular Biology 2020Quote: ... we used TaqMan probes for human HTT and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs obtained from Applied Biosystems. Relative HTT gene expression was determined using the ddCt method ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: Human ISYNA1 isoform 2 (UniProt classification) was cloned into a pcDNA4/TO mammalian expression vector (Thermo Fisher Scientific). The cDNA was amplified by PCR (forward primer ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Genomics 2020Quote: ... LECs were stained with probes designed to target human LETR1 (Type 1 Probe, VA1-3018146, Thermo Fisher Scientific), human Malat1 (positive control ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor 647 anti-human CD137 (clone 4B4-1; dilution 1:100; Thermo Fisher Scientific; Cat no A51019); PE anti-human CD134 (clone OX40 ...
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bioRxiv - Molecular Biology 2021Quote: ... T cells were either frozen or stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (ThermoFisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor genomic copy number was also assessed using the Genome-Wide Human SNP array 6.0 (Thermo Fisher Scientific). DNA prehandling and array hybridization were performed according to the manufacturer’s instructions (Affymetrix ...
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bioRxiv - Physiology 2020Quote: ... RNA isolation of human left ventricular biopsies was performed with TRIzol (Invitrogen/ThermoFisher Scientific Inc., Waltham, MA, USA). The cardiac tissue samples (0.3 – 5.4 mg ...
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bioRxiv - Bioengineering 2019Quote: Human adult fibroblasts were harvested and re-suspended at 1.5×106 cells/mL in dermal proliferation media (ThermoFisher, DMEM-High Glucose supplemented with 1% Pen/Strep ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Bound cynomolgus monkey antibodies were detected with alkaline phosphate-labeled goat-anti-human IgG (1:1000, 4HI1305, Invitrogen Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using Dynabeads Untouched Human T Cells Kit using manufacturer’s protocols (Thermo Fisher, Waltham, MA). Isolated T cells were kept in T cell media ...
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bioRxiv - Genomics 2019Quote: Immortalized human astrocytes (51) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) plus 10% fetal bovine serum (FBS; Invitrogen) on fibronectin-coated (F1141 ...
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bioRxiv - Immunology 2019Quote: ... and anti-human HLA-DR conjugated to eVolve 605 (clone LN3, cat. no. 83-9956-41, Affymetrix-Ebioscience). For bulk RNA-seq datasets ...
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bioRxiv - Microbiology 2019Quote: ... and human monocytic leukemia (THP-1) cells (TIB-202; ATCC) cell lines were maintained in RPMI medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... was analyzed using Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) microarrays after cDNA amplification using GeneChip WT Pico Reagent Kit (Affymetrix) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... were co-cultured on irradiated murine embryonic fibroblasts in human embryonic stem cell medium [DMEM/F12 (ThermoFisher Scientific), 20% Knockout Serum Replacement (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... Human U2OS cells and mouse 3T3 cells were counted using the Countess II automated cell counter (Thermo Scientific) and fixed with 2% formaldehyde using the Arima Hi-C Kit (Arima) ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Human embryonic kidney cells (293T; ATCC; ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), 10% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2021Quote: ... Both human and simian hepatocytes were maintained at 37 °C in 5% CO2 in William’s E medium (Gibco) supplemented with 10% fetal clone III serum (FCS ...
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bioRxiv - Neuroscience 2021Quote: ... and a mouse monoclonal antibody against human KMO (# 60029-1-Ig 1:1000, Thermo Fisher Scientific, Massachusetts, EUA). The membranes were washed and incubated for 1 h at room temperature with an HRP-conjugated secondary antibody (1:10000 ...
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bioRxiv - Biochemistry 2020Quote: CD3/CD28 Dynabeads were removed from stimulated primary human CD4+ T cells using a DynaMag-2 magnet (Invitrogen). For antibody staining ...
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bioRxiv - Bioengineering 2020Quote: The human fetal osteoblast (hOB) 1.19 cell line (ATCC) was expanded at 33.5°C in DMEM/F12 media (Gibco) containing 10% FBS (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Human phospho-tau [pT181] was measured using a commercially available ELISA per manufacturer’s instructions (Thermo Fisher Scientific, KHO0631). Guanidine extracted supernatants of hippocampus homogenates described above were utilized for the pTau ELISA.
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C). Also ...
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bioRxiv - Neuroscience 2020Quote: ... Total Tau, phospho Tau (pS396, pS199, pT231 and pT181) levels were analysed by Human ELISA Kit from Invitrogen. Cerebral organoids and supernatants (for Aβ ...
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bioRxiv - Cancer Biology 2021Quote: MMP1 in the secretome of cell lines was quantified with a MMP1 Human ELISA Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and then with the following secondary antibodies: goat anti-human IgG (H+L) Alexafluor 488 (Invitrogen, 1/200), goat anti-mouse IgG2b Alexafluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Fibroblasts were isolated from normal human skin and cultured in Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal calf serum ...