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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... were purchased from EBISC (European Bank for Induced pluripotent Stem Cells) and maintained on Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific) in Essential 8™ Flex Media Kit (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... with two DT-A gene expression cassettes (maize polyubiquitin 1 promoter + DT-A + rice hsp 16.9a terminator and rice elongation factor-1a promoter + DT-A + rice hsp 16.9b terminator) by a Gateway LR clonase II reaction (Thermo Fisher Scientific), yielding pKOD4/Psog1-GUS_HPT (Figure 1A ...
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bioRxiv - Pathology 2022Quote: ... in 1X Perm buffer (eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set, ThermoFisher, #00-5523-00, in the dark, 45min., RT). Cells were washed in 1X Perm buffer (350g ...
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bioRxiv - Pathology 2022Quote: ... RT) washed in 1X Perm buffer (eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set, ThermoFisher, #00-5523-00, 350g, 5min., RT) and incubated with anti-Ter119 (4µg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Five million C4-2 or two million SK-OV-3 cells in serum free media were mixed one to one with growth factor reduced Matrigel® (Invitrogen) in a total volume of 200 μl and injected in the hind flank using a 23- or 27-gauge needle ...
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bioRxiv - Cell Biology 2022Quote: ... The transcripts of lysosome biogenesis genes and their transcription factors were amplified from the cDNA in QuantStudio 6 Flex real-time PCR system (Applied Biosystems). As a control 18S rRNA or β-Actin were amplified along with the lysosomal genes ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed at room temperature using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific, Waltham, MA) as directed by the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... 100 μg/mL streptomycin with either 110 U/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) (Leukine; Sanofi) and 282 U/mL interleukin-4 (IL-4) (Affymetrix, eBioscience) for DCs or 280 U/mL GM-CSF for macrophages ...
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bioRxiv - Immunology 2020Quote: ... and then fixation and permeabilization using the eBioscience Foxp3/Transcription Factor Staining Buffer Set according to manufacturer’s instructions (Thermo Fisher Scientific). Antibodies used are listed in Table S3 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by fixation in 2% PFA (panel 1 and 3) for 15 min or overnight fixation with eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (00-5523-00, Invitrogen) (panel 2) ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... They were cultured using Keratinocyte-SFM medium with 50 µg/mL bovine pituitary extract and 5 ng/mL epidermal growth factor (ThermoFisher Scientific) in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Genomics 2019Quote: ... Primary bone marrow cells were grown in “C10” media as previously described47 and supplemented with macrophage colony stimulating factor (M-CSF) (ThermoFisher Scientific) at final concentration of 10 ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... diluted by a factor of five into fresh HL-60 media containing one drop of the nuclear dye NucBlue (R37605, Thermo Fisher) per 500 μL ...
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bioRxiv - Cell Biology 2021Quote: ... generated from reprogrammed dermal fibroblasts by retrovirus carrying the Yamanaka gene factors) was maintain on Matrigel-coated culture dishes in Essential 8 Flex Medium (ThermoFisher Scientific). The protocols for culturing cell lines and differentiating the hiPSCs into HBs ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then seeded in laminin-coated tissue culture plates and cultured in the presence of 50 ng/μL nerve growth factor (NGF) (Thermo Fisher) unless otherwise noted.
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bioRxiv - Developmental Biology 2021Quote: ... Pancreatic progenitors differentiated from hESCs at the end of Stage 4 using the described pancreatic differentiation protocol were dissociated into single cells with TrypLE and resuspended in a 3:1 mixture of growth-factor reduced Matrigel and expansion medium (DMEM/F12 Glutamax (Thermo Fisher), 10 μM ROCK inhibitor Y-27632 ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... Monocytes were differentiated into pro-inflammatory (Mφ1) or anti-inflammatory (Mφ2) macrophages with 5 ng/ml of granulocyte-macrophage colony- stimulating factor (GM-CSF; Life Technologies-Invitrogen) or 50 ng/ml macrophage colony- stimulating factor (M-CSF ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were then fixed and permeabilized using Invitrogen’s Transcription Factor Staining Buffer Set (Thermo Fisher Scientific; Cat. No.: 00-5523-00) according to manufacturer’s instructions and stained with Nur77 (12.14 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: BAL and NT samples were stained in 96-wells plates using the FoxP3 / Transcription factor staining buffer set (eBioscience, Thermo Fisher). Cells were stained with α-CD4-APC (02 ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were cultured in basement membrane extract (BME, Trevingen) and overlaid with growth medium consisting of murine recombinant epidermal growth factor (EGF 50 ng/ml, Life Technologies), murine recombinant Noggin (100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Cancer Biology 2022Quote: Organoid growth was evaluated in growth factor–free (GF-free) medium (Advanced DMEM/F12 [Gibco, Thermo Fisher Scientific, Waltham, MA, USA] supplemented with 1x GlutaMax [Gibco]) or StemPro medium (StemPro hESC [Invitrogen] ...
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bioRxiv - Bioengineering 2022Quote: ... and stained with primary antibodies (1:200 CD31; Dako IS610 or 2.5 μg/mL von Willebrand Factor viii; Invitrogen MA5-14029) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed in FACS buffer and fixed and permeabilized with the Foxp3 / Transcription Factor Staining Buffer Set (00-5523-00, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed once with FACS buffer and subsequently fixed for 30 minutes with fixation/permeabilisation buffer of eBioscience Foxp3/transcription factor staining buffer set (ThermoFisher Scientific). They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4 ...
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bioRxiv - Cancer Biology 2024Quote: ... with 50,000 mouse endothelial cells (harvested from mouse lungs by CD31 selection and expanded in vitro as previously described 67) and resuspended in 50 µl of Geltrex™ reduced growth factor basement membrane matrix (A1413301, Gibco). 100 µl of 1:1 GFP+:endothelial cell mixture was plated on transwell inserts with 0.4 µm pores and allowed to solidify for 30 mins in a humidified CO2 incubator (EOE ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Intranuclear and intracellular marker were stained after fixation and permeabilization of cells with the FoxP3/Transcription Factor staining buffer set (Thermo Fisher) or Cytofix/Cytoperm (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Cells that received an intracellular stain were fixed and permeabilized using the FOXP3/Transcription Factor staining buffer set (Thermo Fisher Scientific). Flow cytometry data were collected on the LSRFortessa X-20 ...
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bioRxiv - Immunology 2024Quote: ... Parallel co-cultures were kept for one week to assess expression of FOXP3 and HELIOS (Stability) by intracellular staining using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience, ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were first surface stained as described above and fixed (eBioscience Transcription Factor Staining Set; Thermo Fisher Scientific, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Individual strips with a small amount of medium were then carefully moved to pre-chilled micro-centrifuge tubes and then mixed with cold reduced growth factor basement membrane matrix Geltrex (Thermo Fisher). The dilution of Geltrex by the introduced medium was less than 20% ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized and stained with indicated antibodies using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific). Data were acquired on a BD LSRII or Fortessa cytometer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The collection of HDR enhancing factors fused to MS2 protein were constructed based on the pcDNA™3.1 vector backbone (Thermo Fisher). The following expression vectors were amplified by PCR ...
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bioRxiv - Neuroscience 2023Quote: ... and 62.5 ng of cDNA was amplified using TaqMan probes for vascular endothelial growth factor alpha (Vegfa) and 18S as endogenous control (Life Technologies), and SYBR green probes for cd93 ...
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bioRxiv - Cancer Biology 2023Quote: ... per condition with 200 μl serum-free DMEM supplemented with 20 ng/ml basic fibroblast growth factor (Invitrogen, Grand Island, NY), 10 □ng/ml epidermal growth factor (BioSource ...
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bioRxiv - Cell Biology 2023Quote: ... 30,000 fibroblasts/mesenchymal cells per well) in modified MTEC medium diluted 1:1 in growth factor-reduced Matrigel (cat. no. CB-40230A, Thermo Fisher). Modified MTEC culture medium is composed of SABM with insulin ...
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bioRxiv - Cell Biology 2023Quote: Contact activation and tissue factor-induced thrombin generation times (TGT) were performed in Nunclon Delta Surface 96 well microtiter plates (Thermo Scientific).31 The assay was performed in 25 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... (74) were cultured in keratinocyte serum-free media (KSFM) supplemented with bovine pituitary extract and human epidermal growth factor (Thermo Fisher). HCT116 human colon carcinoma lines were obtained from ATCC and maintained following their recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... spheres were plated as neural progenitors onto a 10cm dish precoated with growth factor reduced (GFR) Matrigel (CB-40230A, Fisher Scientific). Neural progenitors were allowed to form neuronal rosettes and sustained in N2B27 media alone for days 8–15 ...
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were grown also on plates coated Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Immunology 2023Quote: ... the cells were fixed/permeabilized overnight at 4°C in dark using eBioscience FOXP3 transcription factor staining buffer set (cat. #00-5523-00; Thermo Fisher) and FOXP3 monoclonal antibody (1:25 dilution ...
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bioRxiv - Immunology 2023Quote: ... surface-labelled cell suspensions were fixed using eBioscience Foxp3/Transcription Factor Staining Buffer Set or eBioscience IC Fixation Buffer (both from Thermo Fisher). Cell suspensions were stained with following antibodies at 4°C for 30 min ...