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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of all other primary antibodies was performed using a goat anti-mouse Poly HRP secondary antibody or goat anti-rabbit Poly HRP secondary antibody (Invitrogen B40961/2; 10-minute incubation). The HRP-conjugated secondary antibody polymer was detected using fluorescent tyramide signal amplification using Opal dyes 520 ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 hours at RT and followed by a goat anti-mouse Alexa Fluor 488 conjugated secondary antibody (1:500, Molecular Probes, cat# A-11029, RRID:AB_138404) for another 2 hours at RT ...
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bioRxiv - Pathology 2023Quote: Immunohistochemical staining for human claudin-2 was performed on formalin fixed paraffin embedded human pancreas tissue sections by using a mouse-anti claudin-2 (1:100, Invitrogen Cat# 32-5600, RRID: AB_2533085). Pancreas sections were deparaffinized ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were washed thrice with 1× PBS then stained with the secondary antibody goat anti-mouse Alexa Fluor® 555 Plus conjugate (2 μg ml−1, 1:1000-fold dilution; Cat No. A32727, Molecular Probes, ThermoFisher, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Membranes were then incubated with the primary antibodies (listed in Table 2) and HRP-linked secondary antibodies (anti-mouse or anti-rabbit IgG; Thermo Fisher Scientific, 10368172 and 11034). Clarity Western ECL (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed once with 2% HI-FBS S-MEM and further stained with PE-conjugated anti-mouse IgM (Thermo Fisher Scientific, #12-5790-81) for 30 minutes to detect the HTII-280 primary antibody ...
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bioRxiv - Immunology 2024Quote: ... incubated at room temperature for 2 hours with Alexa Fluor® 647-conjugated donkey anti-mouse IgG (H+L) antibodies (1:500, Invitrogen, # A-31571, RRID: AB_162542) and 0.8 µM Hoechst 33342 at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and FURA 2-AM (Fura-2-acetoxymethyl ester) from Invitrogen, CA ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μM oligodT (dT25) and 2 μM random hexamers (Invitrogen) or 50nM stem-loop specific primers (for miRNA expression analysis) ...
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bioRxiv - Bioengineering 2019Quote: ... 50 μM Gibco 2-Mercaptoethanol (2-ME) (Thermo Fisher, 21985023), 1x antibiotic-antimycotic (anti-anti ...
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bioRxiv - Cell Biology 2021Quote: ... A fluorescent derivative of 2-deoxyglucose (2-NBDG, Invitrogen, N13195) was added at a final concentration of 20 µM for a further 30 min incubation at 37 °C ...
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The structure of insulin granule core determines secretory capacity being reduced in type-2 diabetesbioRxiv - Physiology 2022Quote: ... Nucleobindin 2 and siRNA control #2 were purchased from ThermoFisher Scientific ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... 2 mM L-Gln and 50 μM 2-mercaptoethanol (Invitrogen).
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bioRxiv - Genetics 2022Quote: ... 2% B-27 and 1% N-2 supplements (Life Technologies) and supplements 20ng/ml BDNF ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were loaded with 2 μM Fura-2/AM (Invitrogen). Ratiometric Ca2+ imaging was performed at 340 and 380 nm in 0 or 2 mM Ca2+ Ringer’s solution using an IX81 microscope (Olympus ...
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bioRxiv - Cell Biology 2019Quote: ... 2 µg of DNA and 2 µl Plus reagent (Invitrogen) were added to 1 ml serum-free media in a centrifuge tube ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME, Thermo Fisher Scientific)) supplemented with 4 ng/ml bFGF ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM L-glutamine and 2% penicillin/streptomycin (ThermoFisher Scientific). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with 2 μM Fura-2 (F1221, Invitrogen) at 37℃ for 30 min dispersed in Margo solution with 0.2‰ w/v Pluronic F-127 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µL FastAP Thermosensitive Alkaline Phosphatase (2 U) (Thermo Scientific) was added and the solution was incubated at 37°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... A 2 × 2 cm piece of Parafilm M (Fisher Scientific) was carefully placed onto the tissue so that the gene panel mix was evenly spread across the tissue and no bubbles were introduced between the tissue and the film ...
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bioRxiv - Cell Biology 2024Quote: ... containing 2% of 2-mercaptoethanol (Thermo scientific Cat. No: 125470100) and heated at 70°C for 10 mins before freezing them at – 80°C ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME, Thermo Fisher Scientific), supplemented with 100 ng/mL Activin A (Nacalai Tesque) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μg mL−1 puromycin (58-58-2, ≥98%, Invitrogen) and 100 μg mL−1 hygromycin (31282-04-9 ...
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bioRxiv - Immunology 2024Quote: ... followed by interleukin 2 (IL-2) (20 ng/mL, Gibco) stimulation for 5 days.
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bioRxiv - Bioengineering 2024Quote: ... 2-Nitrobenz Oxadiazol Amino Deoxyglucose (2-NBDG, Invitrogen, Cat# N13195) for 30 minutes (figure 4(a)) ...
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bioRxiv - Cell Biology 2023Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... with 2 pulses of 950V lasting 2 ms by Invitrogen Neon Transfection system (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... 2 mL sample was preserved in 2 mL RNAlater (Invitrogen), stored at 4°C until all national samples were collected (usually within a few days) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 % penicillin-streptomycin) supplemented with 2% N-2 supplement (Gibco), 1% B-27 supplement serum free (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were then enriched via negative selection using Dynabeads Untouched Mouse CD4 T cells Kit (Thermo Fisher Scientific) according to manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... mice which were maintained on tamoxifen diet for 4-5 wks and from CD45.1 mice (as described above) which were subjected to T cell depletion using Dynabeads FlowComp Mouse Pan T (CD90.2) Kit (ThermoFisher Scientific). BM cells from WT or FL mice were mixed with the BM of the competitor donor at 1:1 ratio and then transplanted by intravenous injection into lethally irradiated CD45.1 mice (900 rad) ...
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bioRxiv - Microbiology 2020Quote: Luminex profiling was performed on whole blood that was allowed to clot for 20 minutes and then spun down using a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and then stained with triple-washed anti-IgG magnetic beads (Untouched Mouse T Cells Kit, Thermo Fisher) rotating at 4°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: Using a subcloned and sequenced PCR fragment (162 bp – Table S1) of the mouse 5-HT2AR cDNA (Zero Blunt TOPO PCR Cloning kit, Invitrogen), high specific-activity RNA probes were produced from a linearized plasmid (HindIII ...
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bioRxiv - Microbiology 2022Quote: Chemokine and cytokine profiles of serum and lung samples were assessed using Immune Monitoring 48-plex mouse ProcartaPlex Panel kits (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... containing a 1/250 dilution of Alexa 488-conjugated anti-Spike antibody (GTX632604 conjugated using the Zenon Alexa Fluor 488 Mouse IgG Labeling Kit, ThermoFisher) and washed 4 times in FACS buffer ...
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bioRxiv - Physiology 2021Quote: ... RNA samples were prepared from the mouse tissues using the PureLink RNA Mini Kit (Invitrogen by ThermoFisher Scientific, catalog #12183025). Briefly ...
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bioRxiv - Immunology 2020Quote: Peripheral T cells were isolated from mouse spleen by a CD4+ T-cell negative (Stem Cell) or positive selection kit (Invitrogen). Enriched CD4+ T cells were further sorted for either YFP+ (Foxp3+ ...
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bioRxiv - Neuroscience 2020Quote: ... were designed against the mouse Auts2 coding sequence using the BLOCK-iT Pol II miR RNAi Expression Vector Kit guidelines (Invitrogen). Auts2 miRNA constructs were subcloned into the pCL20c-trL7 ...
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bioRxiv - Immunology 2020Quote: Splenic T cells from MRL/Lpr mice were purified with Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit and dissocated from beads as per manufacture’s instructions (ThermoFisher). Purified T cells were staind with PE-conjugated anti-CD138 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... For direct labeling of the anti-CC10 antibody Zenon Alexa Fluor 546 mouse IgG1 kit was used according to the manufacturer’s recommendations (Invitrogen, 25004). Stained cryosections were analyzed on an epifluorescence microscope (Zeiss Imager.M2 ...
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bioRxiv - Immunology 2021Quote: ... S98I to the SIRPα domain which was then subcloned and fused to mouse IgG1 N297A via GeneArt Seamless Plus Cloning Assembly Kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Barcoded libraries were prepared using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit as per the manufacturer’s protocol and sequenced using an Ion S5 system (ThermoFisher Scientific). Differential gene expression analysis was performed using the Transcriptome Analysis Console (TAC ...
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bioRxiv - Immunology 2021Quote: MesLNs were collected and CD4+ T cells isolated using the Dynabeads™ Untouched™ Mouse CD4 Cells Kit (Thermo Fisher) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... naïve OT-I and OT-II cells were obtained by negative selection using the Dynabeads Untouched Mouse CD4 cell kit (ThermoFisher). Flow cytometry indicated >85% enrichment in all cases ...