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Citations for Thermo Fisher :
3351 - 3400 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Western blot analysis was conducted using cytoplasmic extracts from mouse brain cells made with a NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher). The mixture was centrifuged at 10,000□×□g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from HeLa cells (human) or melanocytes (mouse) melan-Ink4a (Shakya et al., 2018) was isolated using a GeneJET RNA purification kit (ThermoFisher Scientific) in the presence of β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... We simultaneously determined the concentrations of cytokines in supernatant samples using a customized 28-plex immunoassay kit (ProcartaPlex Mouse 28-plex, ThermoFisher Scientific), which employs magnetic beads for detection ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells from OT-I donors were enriched using the Dynabeads® Untouched™ Mouse T Cells kit (Invitrogen) and stained in 1ml DPBS with CellTrace Violet dye (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Cytokines were quantified from 1 ml right lung homogenate using the ProcartaPlex Mouse 12-Plex Mix&Match kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: RNA Isolation: RNA extraction and isolation of mouse podocytes was performed using the PicoPure RNA isolation Kit (Thermo Fisher Scientific, #KIT0204) according to manufacturer’s protocol (100 µL instead of 50 µL of lysis buffer was applied to extract RNA) ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated from cultured mouse neurons treated with xcTauOs (250 nM total tau) or vehicle for 6 hours using the mirVana isolation kit (Invitrogen, AM1560). RNA integrity was evaluated by capillary electrophoresis using an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... ribosomal RNA was depleted from the samples using the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific, Waltham, MA). All samples were sequenced on an Illumina Hi-Seq sequencer to produce 150 bp paired-end reads ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... from 150 μl plasma (single mouse or pooled) and the TG and cholesterol content were measured using colorimetric kits (Inifinity, Thermo Scientific, TG catalog #TR22421 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked again and incubated with 2-3 drops of an anti-mouse poly-HRP-conjugated secondary antibody from the Invitrogen Tyramide SuperBoost secondary staining kit (ThermoFisher, B40912). Samples were rinsed three times with 1X PBS and the tyramide working solution conjugated to AlexaFluor 488 was added and allowed to react for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched using negative magnetic selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D).
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bioRxiv - Neuroscience 2023Quote: Mouse embryonic fibroblast (MEF) cell lysates were processed using the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Initial isotyping of the antibodies in hybridoma supernatants was carried out using Pierce Rapid Antibody Isotyping Kits for mouse (Thermo Fisher). Antibodies were subsequently used as confluent supernatants for most techniques ...
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bioRxiv - Microbiology 2019Quote: ... goat-α-mouse-Alexa-488 and goat-α-mouse-Alexa-594 (Life Technologies). FISH was performed using a PNA probe specific to the telomeric sequence (CCCTAA)3 (TelC-Cy5 ...
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bioRxiv - Systems Biology 2024Quote: ... Incubations were performed with mouse anti-FLAG or mouse anti-V5 (ThermoFisher Scientific), and secondary anti-mouse IgG coupled with alkaline phosphatase (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and treated with 1x ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used were against mouse (Donkey anti-mouse, AF488; Invitrogen Cat#A21202) or goat (Donkey anti-goat AF488 ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Developmental Biology 2021Quote: ... in myoblast growth medium (HAM’s F-10 medium with 20% FBS, 10 ng/ml basic fibroblast growth factor (Thermo Fisher Scientific, PHG0263), penicillin (100 U/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... Mitogenic stimulus conditions were applied immediately after irradiation and consisted of control medium with the addition of 1 ng/mL TGF-β1 and 5 ng/mL basic fibroblastic growth factor (bFGF) (PHG9204 and PHG0264; Thermo Fisher Scientific). In additional experiments with human cartilage ...
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bioRxiv - Immunology 2022Quote: ... and then stained with anti–Foxp3 at 23°C for 30 min using a Foxp3/Transcription transcription factor buffer set (Invitrogen, ThermoFisher Scientific). Samples were acquired with BD FACSCelesta (BD Biosciences) ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were re-suspended in ECGM containing 10% FBS and 40 ng/ml basic fibroblastic growth factor (bFGF; Thermo Fisher Scientific-Invitrogen, UK) and seeded at 5 × 104 cells/well (n=6) ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... fixed and permeabilized cells following manufacturer’s instructions for the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA, USA), barcoded samples using mass-tag cellular barcoding reagents diluted in Maxpar Barcode Perm Buffer (Fluidigm ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies and the dilution factors were as follows: goat anti-rabbit IgG Alexa Fluor 488 conjugate (A-11034, Thermo Fisher Scientific) 1:250 ...
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bioRxiv - Biophysics 2020Quote: ... and 1% bovine serum albumin (BSA) and then incubated with biotinylated Epidermal Growth Factor (EGF) complexed to Texas Red™ Streptavidin (ThermoFischer/Molecular Probes Cat ...
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bioRxiv - Cancer Biology 2020Quote: ... with serum-free DMEM supplemented with basic Fibroblast Growth Factor (20 ng/mL) and B-27 supplement (1:50; Thermo Fisher Scientific). Media was supplemented on Day 4 ...
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bioRxiv - Cell Biology 2022Quote: ... Human iPSC lines were cultured feeder-free on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix and maintained in Essential 8 Flex Basal Medium (Thermo Fisher Scientific) supplemented with Essential 8 Flex Supplement (50x ...
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bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: iPSCs were cultivated for several passages under feeder-free conditions (in the Essential 8™ Medium on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific). 3-4 days before differentiation ...
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bioRxiv - Biophysics 2019Quote: ... iPSCs were cultured in the Essential 8™ Medium on a Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific) after transferring from the feeder layer ...
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bioRxiv - Immunology 2020Quote: ... containing macrophage colony-stimulating factor (M-CSF) was generated by culturing L929 cells in RPMI 1640 medium with L-Glutamine (Thermo Fisher Scientific) containing 10 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... was subjected to quantitative real-time PCR (qPCR) to measure the mRNA levels of cytokines and transcription factors using a SYBR Green PCR Master Mix (Applied Biosystems, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained for intracellular cytokines and transcription factors in 50 μL 1X eBioscience Permeabilization Buffer (ThermoFisher Scientific: 00-8333-56) at 4°C for at least 1 hr ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured and expanded in 6-well plates coated with Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (A1413202, Gibco Life Technologies) diluted 1:100 in DMEM (31966021 ...
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bioRxiv - Microbiology 2020Quote: ... or an equal volume of 100% acetone were mixed with an equal volume of Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific). The mixture was applied onto a glass slide ...
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bioRxiv - Molecular Biology 2022Quote: iPSCs cell line WTSli002 purchased from EBISC (European bank for induced pluripotent cells) were maintained on feeder-free conditions on Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific, A1413302) in Essential 8™ Flex Media Kit (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... and the pellet resuspended in Keratinocyte Serum Free Media supplemented with Epidermal Growth Factor 1-53 and Bovine Pituitary Extract (Life Technologies; 17005042). Cells were either live/dead sorted using SYTOX Blue Dead Cell Stain (ThermoFisher ...
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bioRxiv - Systems Biology 2022Quote: Primary human keratinocytes were cultured in Keratinocyte Serum Free Media supplemented with Epidermal Growth Factor 1-53 and Bovine Pituitary Extract (Life Technologies; 17005042). Generation of organotypic skin cultures were performed as described in Li and Sen ...
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bioRxiv - Immunology 2022Quote: ... or corresponding isotype control antibodies diluted in FACS buffer for 30 min at 4 ° After fixation and permeabilization for 30 min at 4 °C using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), resuspended cells were kept overnight at 4 °C ...
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bioRxiv - Systems Biology 2022Quote: ... culture media was switch to Keratinocyte Serum Free Media supplemented with Bovine Pituitary Extract and variable concentrations of Epidermal Growth Factor 1-53 (Life Technologies; 17005042) after one week for one additional week of culturing.
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bioRxiv - Synthetic Biology 2024Quote: ... All antibodies were diluted in blocking buffer as described in the Bioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00).
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bioRxiv - Microbiology 2023Quote: ... and pC-L (1 μg) that supply the trans-acting factors LCMV NP and L 70 using 10 μL of Lipofectamine 2000 (Thermo Fisher Scientific) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: Day 9 trunk NC cells derived from hESCs as described above were harvested and plated at a density of 500 cells/cm2 in plates pre-coated with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat# A1413202, Thermo Fisher Scientific) in the presence of DMEM/F12 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μM of Rock Inhibitor (Y-27632) on Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat# A1413202, Thermo Fisher Scientific). 48 hours later (D5) ...
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bioRxiv - Immunology 2023Quote: ... cells were instead fixed and permeabilized with a 1X FoxP3 Fixation/Perm buffer (eBioscience FoxP3/Transcription Factor Staining Buffer Set, Thermo Fisher Scientific) for 30 min at 4 °C ...