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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The amplified products were checked on 2% agarose electrophoresis and purified using Purelink PCR purification kit (Invitrogen, United States). The purified amplicons were quantified on Qubit 2.0 using qubit dsDNA BR assay kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... putida from 2 mL of overnight LB medium cultures with the PureLink Genomic DNA Mini kit (ThermoFisher Scientific Co.). Library construction ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 2 µg of total RNA using a TRUE Script Reverse Transcription Kit (+gDNA Eraser) (Invitrogen). Quantitative real-time PCR was performed on an ABI Quant Studio™ 6 Flex system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of total RNA was used to synthesize cDNA using the High Capacity Reverse Transcriptase kit (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of total RNA was used to synthesize cDNA using the High Capacity Reverse Transcriptase kit (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was purified from 2 ml of cultures using the GeneJET Genomic DNA Purification Kit (K0722, Thermo Scientific) in accordance with manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Fura-2 fluorescence was calibrated with defined Ca2+ buffers generated with the Ca2+ Calibration Kit #1 (Invitrogen, Waltham, USA).
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bioRxiv - Plant Biology 2023Quote: ... determined by qPCR with the KAPA kit (Biosystems. Boston, MA, USA) and confirmed with a 2-point Qubit 2.0 fluorometer (Invitrogen). The library was denatured with 10 µL of 0.2N NaOH and diluted in a solution of denatured PhiX ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 3’ end RNA was synthesized by in vitro transcription using an mMESSAGE mMACHINE T7 kit (Invitrogen) with a DNA template (5’- TAATACGACTCACTATAGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAUGACGTAGCATGCTACGCG -3’ ...
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bioRxiv - Molecular Biology 2022Quote: ROS levels were detected using the chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) general oxidative stress indicator kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 2 µg of RNA template using the High Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems). For the qPCR reaction mixture KAPA SYBR FAST Master Mix (2x ...
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bioRxiv - Plant Biology 2024Quote: The cDNA samples were synthesized from 2 µg of RNA using a High-capacity cDNA synthesis kit (Applied Biosystems). RT-qPCR was performed using the ABI SYBR green kit as per manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% [v/v] 2-mercaptoethanol) and then quantified with the Pierce BCA protein assay kit (Thermo Fisher Scientific). The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... RNA (2 μg) was reverse transcribed using random primers with a RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific). Quantitative PCR was performed in triplicate using the SYBR Green method (Life Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... Tris (2-carboxyethyl) phosphine hydrochloride (TCEP.HCl) and Vybrant™ CFDA SE Cell Tracer Kit were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNAs were prepared from 2 μg RNA with random hexamers using the High-Capacity cDNA Synthesis Kit (Thermo Scientific), followed by RT-PCR to identify the BCR::ABL1 fusion transcripts using a previously described protocol (17) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis from 1.5 or 2 μg RNA was performed using the High Capacity cDNA RT Kit (Applied Biosystems). Quantitative RT-PCR experiments were run on a C1000 Touch Thermal Cycler (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of the total RNA was converted into cDNA using the SuperScript II Reverse Transcriptase kit (Invitrogen™). qPCR was performed on CFX384 Real- Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared with 2 µg of total RNA using RevertAid first strand cDNA synthesis kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... 2 µg of RNA was used for cDNA synthesis using a high-capacity cDNA reverse transcription kit (Applied Biosystems). PCR products were checked using Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was prepared from 2 µg RNA using the High-capacity cDNA Reverse Transcription kit (Applied Biosystems, Cat# 4368813) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were blocked using PBS supplemented with 3% bovine serum albumin (PBSA; Thermo Fisher Scientific, Waltham, MA). THL-treated virus was added to blocked KZ52-coated plates and allowed to adsorb at 37°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times before incubation with 1x TMB ELISA substrate solution (eBioscience; Thermo Fisher Scientific, Inc.) and the reaction was stopped with 1 M H2SO4 ...
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bioRxiv - Neuroscience 2020Quote: ... Human phospho-tau [pT181] was measured using a commercially available ELISA per manufacturer’s instructions (Thermo Fisher Scientific, KHO0631). Guanidine extracted supernatants of hippocampus homogenates described above were utilized for the pTau ELISA.
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bioRxiv - Neuroscience 2020Quote: The level of Aβ in the tissue was assessed by ELISA as per the manufacturer’s instructions (KHB3442, Invitrogen). The resulting measurements were normalized for tissue weight.
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bioRxiv - Microbiology 2022Quote: Headless HA ELISAs were performed using 96-well Immunlon 4HBX flat-bottom microtiter plates (no. 3855; Thermo Fisher) coated with 0.5 ug/well of streptavidin (no ...
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bioRxiv - Neuroscience 2021Quote: ... were prepared for ELISAs by coating each well with 50ul of 1.5ug/ml HT7 monoclonal antibody (Thermo Fisher) the night before use ...
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bioRxiv - Neuroscience 2020Quote: ... whole brain lysates were prepared as for ELISA and protein concentration determined using a BCA assay (Thermo Fisher). Wells were loaded with 30 µg of total protein which was resolved by SDS-PAGE under reducing conditions and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: ELISA was performed using anti-GST coated strips (Pierce™ Anti-GST Coated Clear Strip Plates, Thermo Scientific). A fixed amount of 10 pmol GST-tagged PA1217 was diluted in 200 µl PBS (137 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... and results of the ELISA were measured by a colorimetric microplate reader (VarioSkan 2.2; Thermo Scientific Inc., USA) at 450nm ...
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bioRxiv - Pathology 2021Quote: ... After staining with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, San Jose, CA, US), mean absorbances in duplicates were read at 450nm and ratio of HAA/PNA was calculated for each sample in order to measure changes of glycosylation ...
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bioRxiv - Microbiology 2021Quote: ... Cells were pelleted (400xg, 10 min) and supernatants subjected to ELISA analysis for IL-17a (Thermo Fisher, UK).
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... CRP and IL-6 were measured in UCB plasma using a high-sensitivity ELISA (Life Technologies, Carlsbad CA) per the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... and the assay was developed with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher, Cat#34029) and blocked with 2N H2S04 solution before reading ...
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bioRxiv - Immunology 2022Quote: ... 200 ng/ml) and medium was collected for analysis of the amount of secreted TNFα by ELISA (Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The concentration of Aß40 and Aß42 in the culture medium was quantified by ELISA (Thermo Scientific, Waltham, MA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well ...
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bioRxiv - Neuroscience 2023Quote: ... the substrate of the peroxidase (100µL, 3,3’,5,5’-tetramentylbenzidine solution; 1-StepTM Ultra TMB-ELISA, 34029, Thermo Scientific) was added in each well for 10 to 25 minutes at RT and in darkness ...
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bioRxiv - Bioengineering 2023Quote: ... 2ug/mL of SARS CoV2 BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Total IgA and IgG ELISAs were performed using 384-well flat-bottom MaxiSorp plates (ThermoFisher, Cat No. 464718) coated with either an unconjugated anti-human IgA (Novusbio ...
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bioRxiv - Immunology 2023Quote: ELISA plates (Microtitre) were coated with 50 µl/well of 5 µg/ml NeutrAvidin (Thermo Fisher Scientific, #31000) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of IL33 and periostin in culture supernatants were measured by ELISA according to the manufacturer’s instructions (Invitrogen, cat # ...
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bioRxiv - Microbiology 2023Quote: ... Plates were developed with 1-Step ultra TMB-ELISA substrate solution (Thermo Fisher Scientific, Waltham, MA, USA, #34028) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Immunology 2023Quote: ... the ELISA plates were incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (1:5000, Invitrogen) for 1 hour at 37°C ...