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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disordersbioRxiv - Molecular Biology 2023Quote: ... fixed with 4% paraformaldehyde (Affymetrix) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 4-well chamber slides (Nunc Lab-Tek II Chamber Slide System ...
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bioRxiv - Developmental Biology 2023Quote: ... using 4-well plates (Nunc) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... or Fluo-4 (F14201 Invitrogen) at 1 µM for 30 minutes or MitoTracker red (M22425 Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 mM L-glutamine (ThermoFisher) and 1 mM sodium pyruvate (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: ... formalin (Fisher Scientific SF98-4), SG-209 (T24786 ...
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bioRxiv - Bioengineering 2022Quote: ... and 4 mM Glucose (Gibco). The enhanced maturation medium 2/1 (EMM2/1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 4% Insulin,-Transferrin-Selenium (Invitrogen), 100 U/ml penicillin and 100 U/ml streptomycin (Hyclone ...
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bioRxiv - Genomics 2023Quote: ... and Qubit 4 fluorometer (Invitrogen), and used 1 ng for DNA library preparation using the Nextera XT DNA Library Preparation kit (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... collagenase type 4 (Fisher Scientific), and dispase (Corning) ...
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bioRxiv - Neuroscience 2023Quote: ... 4 mM Glutamax (Gibco, 35050061) and 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 μg/mL Puromycin (Invitrogen) was used to select cells ...
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bioRxiv - Bioengineering 2023Quote: ... 4°C DMEM/F12 (Gibco). Crypts were centrifuged at 4°C for 5 minutes again and resuspended again with 300 μL DMEM/F12 medium ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM glutamine (Gibco, #25030) 25 mM glucose (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... using fluo-4 (Invitrogen F14200) and a commercial calcium calibration buffer (Invitrogen C3008MP) ...
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bioRxiv - Cell Biology 2024Quote: ... L-glutamine (4 mM, Gibco), sodium pyruvate (1 mM ...
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bioRxiv - Genomics 2024Quote: ... 4 mM deoxynucleoside triphosphates (Invitrogen), 1% bovine serum albumin ...
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bioRxiv - Cancer Biology 2024Quote: ... and 4% PFA (Fisher Scientific) was added to the lungs via intratracheal instillation ...
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bioRxiv - Molecular Biology 2024Quote: ... and Qubit 4 Fluorometer (Invitrogen). Equal amounts of MboI-digested and Hin1II-digested libraries from both Control and t-2-hex samples ...
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bioRxiv - Genomics 2024Quote: ... 4 mM l-glutamine (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluo-4 AM (Life Technologies-Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: FM 4-64 (ThermoFisher, T13320) was used to label hair cells in the crista within the zebrafish inner ear ...
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bioRxiv - Immunology 2024Quote: ... DispaseII (4 U/ml; Gibco) and 5% FBS was injected into the lung cavity ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with secondary anti-mouse AlexaFluor633-conjugated antibody (dilution 1:200 in incubation buffer; Molecular Probes, cat #: A-21052, Eugene, OR, USA) for 1 h in a darkened chamber at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies and the optimal dilutions and concentrations used are as follows: AF647-conjugated donkey anti-mouse IgG (Thermo Fisher, A-31571, 1:500, 4 µg/mL), AF647-conjugated donkey anti-rabbit IgG (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ZO-1 (mouse monoclonal, Invitrogen 33-9100, 1:500), rabbit anti-aPKC (rabbit polyclonal ...
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bioRxiv - Immunology 2020Quote: ... mouse IFNβ (PBL Biomedical Laboratories) and mouse CXCL10 ELISA (ThermoFisher; BMS6018MST) were obtained from commercial sources.
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bioRxiv - Developmental Biology 2023Quote: ... Mouse aggregation medium (mouse AM) consisted of 1x DMEM (GIBCO-41965) supplemented with 20% FBS (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-©tubulin (mouse monoclonal, MilliporeSigma T6557, 1:200) and mouse anti-ZO-1 (Life Technologies, 1:300). Rabbit anti-Pard3 (rabbit polyclonal ...
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bioRxiv - Biochemistry 2020Quote: ... The fluorogenic probe 7-diethylamino-3-(4’-maleimidylphenyl)-4-methylcoumarin (abbr. CPM, ThermoFisher, Cat# D346) in 100% DMSO was then added to both quench the enzymatic reaction and react with CoASH to yield the fluorescent CPM-SCoA complex for fluorescence quantification 27 ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were incubated overnight at 4°C with primary Phospho-Smad 2 (ThermoFisher; #4-244G) antibody diluted 1:200 in blocking buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were fixed overnight at 4° C temperature in 4% paraformaldehyde in PBS buffer (Invitrogen) and incubated in 70% ethanol solution after three times’ wash with PBS buffer for paraffin section ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Cancer Biology 2023Quote: MOLT-4 Cells were loaded with the fluorescein Ca2+ probe Fluo-4 AM (Invitrogen # F14217). Cells were incubated for 60 min in RPMI medium and supplemented with 1 µM Fluo-4/AM ...
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bioRxiv - Neuroscience 2023Quote: ... supplements and Fluo-4 acetomethylester (Fluo-4-AM) were from Life Technologies (Carlsbad, CA, USA). Culture plates were from Costar (Tewksbury ...
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bioRxiv - Physiology 2023Quote: Isolated atrial myocytes were stained with 4 µM di-4-ANEPPS for 10 minutes (Invitrogen) for membrane visualisation[3 ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight and then loaded with Fluo-4 (1:1000; Fluo-4 Calcium Imaging Kit; Invitrogen) and CellTracker Red (1:2000 ...
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bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Developmental Biology 2021Quote: Coding sequence for sbp2 was PCR amplified from 0-5 dpf pooled cDNA using the forward and reverse primers shown in Table 1 and then TA cloned into pcDNA3.1 V5/His Topo (Thermo Fisher Scientific). For secisbp2l ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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bioRxiv - Molecular Biology 2020Quote: Approximately 4 x 105 HEK293T cells in a 6-well plate were transfected with 250 ng/well of pMT107-octa-6 His-ubiquitin or 200 ng/well of pcDNA3-2x-6His-ubiquitin constructs using 50 µl/well Opti-MEM (Gibco; 31985062) and 2 µl/well of FuGENE 6 Transfection reagent (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
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bioRxiv - Neuroscience 2020Quote: ... The genomic titers of DNase-resistant recombinant AAV particles were determined after alkaline treatment of virus particles and subsequent neutralization by qPCR using the qPCRBIO SY Green Mix Hi-Rox (Nippon Genetics Europe GmbH) and an ABI PRISM 7900HT cycler (Applied Biosystems). Vectors were quantified using primers specific for the GCaMP6s sequence (5’-CACAGAAGCAGAGCTGCAG and 5’-actggggaggggtcacag) ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA fragment encoding the full-length Npbwr2 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The resulting Npbwr2 expression construct was transiently transfected into HEK293T cells together with the cAMP-responsive luciferase reporter vector pGL4.29 (Promega ...
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bioRxiv - Pathology 2019Quote: ... The pooled PCR products were added to 9.9 μL of Hi-Di formamide and 0.1 μL of GeneScan™ 500 LIZ™ size standard (Life Technologies). Samples were denatured at 94 C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Emulsion PCR and Ion Sphere Particles (ISP) enrichment were done using the Ion PGM Hi-Q Kit for 200bp (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...