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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Whole-cell protein lysates were prepared in M-PER buffer (Thermo Scientific, Rockford, IL, USA) with 1x Protease & Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: Sandwich ELISA was used to establish plasma concentrations of IL-18 (Invitrogen, Thermofisher, BMS618-3) and ferritin (Abcam ...
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bioRxiv - Immunology 2024Quote: Sandwich ELISA was used to establish plasma concentrations of IL-18 (Invitrogen, Thermofisher, BMS618-3) and ferritin (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The ligation products (5 μl) were transformed into DH10B Competent Cells (Thermo Scientific, Rockford, IL) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined employing the PierceTM BCA protein quantification kit (Thermo Scientific, IL, USA). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were mounted on gelatin-coated glass slides (SuperFrost Plus, ThermoFisher Scientific, IL, US), rehydrated ...
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bioRxiv - Neuroscience 2023Quote: ... CA) and Supersignal West pico PLUS Chemiluminescent Substrate was purchased from Thermo Scientific (Rockford, IL). Aurum Total RNA Mini Kit ...
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bioRxiv - Cell Biology 2023Quote: ... IL-1R2 concentrations were determined in culture supernatants by an ELISA assay (EHIL1R2, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Poland) and by Western blotting using Pierce High Sensitivity-streptavidin-HRP (Thermo Scientific, Rockford, IL) diluted 1:40,000 in 3% BSA/TBST.
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5) added with a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, IL, USA). Protein samples were separated by 10% SDS-polyacrylamide gel electrophoresis (20 µg/lane) ...
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bioRxiv - Microbiology 2023Quote: IL-33 (residues 112–270) and its R169A mutant were cloned into vector pET28a (Invitrogen) and were expressed with a TEV protease cleavable N-terminal His6 tag ...
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bioRxiv - Immunology 2023Quote: ... Enzyme-linked immunosorbent assays (ELISA) were performed to detect IL-1β (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Scientific; Rockford, IL). Equal amounts of lysed extracts were loaded on a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed with Pierce Radioimmunoprecipitation (RIPA) buffer (cat. no. 89901, Thermo Scientific, Rockford, IL) with protease and phosphatase inhibitors ...
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bioRxiv - Immunology 2023Quote: ... Enzyme-linked immunosorbent assays (ELISA) were performed to detect IL-1β (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... with or without recombinant IL-1β (50ng/ml)(Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) for 24hr [45 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 250U Pierce Universal Nuclease for Cell Lysis (ThermoFisher Scientific, Rockford, IL), cells counted on Beckman Vi-Cell XR Cell Counter ...
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bioRxiv - Cell Biology 2023Quote: ... IL) were cultured in Dulbecco’s minimum essential medium (DMEM) high glucose with 10% FBS (Thermofisher) and 100 μg/ml penicillin/streptomycin (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... and Cas9 (PNA Biosciences Cas9 Protein with NLS, NC1279639, Fisher Scientific, LLC, Chicago, IL, USA) were injected into 240 embryos (240 separate embryos per gRNA ...
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bioRxiv - Genomics 2023Quote: ... Gels were stained with the GelCode® Blue stain reagent (Thermo Scientific, Rockford, IL, USA) after fixation using 50% methanol (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... The bands were visualized using west pico chemiluminescent kit (#34580, Thermo Fisher Scientific, Rockford, IL) under chemi-doc touch imaging system ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific binding was blocked by Starting Block T20 PBS Blocking Buffer (Thermo Scientific, Rockford, IL) for 1 hour followed by primary antibodies targeting EV membrane protein ...
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bioRxiv - Microbiology 2023Quote: ... EV protein content was measured using Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Immunology 2023Quote: ... IL-5 levels were quantified in undiluted BAL fluid by ELISA following manufacturer’s instructions (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... and mounted with a coverslip using ProLong Gold antifade mounting media (Thermo Fisher, Rockford, IL). Images were acquired using a Nikon A1 Confocal microscope (Nikon TE2000 inverted microscope provided by the UAB Microscopy Core).
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bioRxiv - Cancer Biology 2024Quote: ... + 500 U/mL Gibco™ recombinant human (rh) IL-2 (Thermo Fisher Scientific, cat# PHC0021). For primary NK cells ...
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bioRxiv - Immunology 2024Quote: Murine TNF and IL-1β were quantified in cell supernatants using the ELISA™ Kit from Invitrogen and human IL-1β was similarly quantified with the Instant ELISA™ Kit from Invitrogen, following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and 100U/ml PeproGMP™ Human IL-2 Recombinant Protein (Thermo Fisher; 200-02-1MG). The T cell culture was transferred to G-Rex bioreactor vessels (Wilson Wolf ...
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bioRxiv - Biochemistry 2024Quote: Pierce LC-MS grade water and formic acid were purchased from Thermo Scientific (Rockford, IL). LC-MS grade acetonitrile and 2-propanol were purchased from Honeywell (Charlotte ...
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bioRxiv - Neuroscience 2024Quote: ... Bound cytokine was detected using a biotinylated IL-6 detection antibody (ThermoFisher Scientific, MP5-32C11), streptavidin horseradish peroxidase (strep-HRP) ...
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bioRxiv - Plant Biology 2019Quote: ... pEF1-MCS-3Myc [BamHI-NotI-3Myc-stop fragment was inserted between KpnI and XbaI sites of pEF1/myc-His B vector (Invitrogen)] was generated ...
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bioRxiv - Microbiology 2020Quote: The parB genes were recombined into a Gateway-compatible destination vector pET-His-MBP-TEV-DEST 56 via an LR recombination reaction (ThermoFisher). For LR recombination reactions ...
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bioRxiv - Developmental Biology 2021Quote: ... was modified by replacement of the SV40 promoter by the Drosophila actin promoter from the pAct5.1/V5-His C vector (Thermo Scientific), and the firefly luciferase coding sequence by the Renilla luciferase (RLuc ...
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bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Immunology 2022Quote: Recombinant soluble ICAM-1 protein was expressed in a Drosophila Melanogaster cells using pMT/V5-His vector with inducible promotor (Invitrogen), purified by sequential two-step affinity chromatography on anti-ICAM-1 antibody (clone YN1.1 ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was cloned between 5’ KpnI and 3’ EcoRI sites of the pMT/V5-His vector (Invitrogen). In-frame Tag-RFP-T gene was then introduced at the 3’ end of γ-tubulin gene between 5’ EcoRI and 3’ NotI sites ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was then inserted into the 5’ SpeI and 3’ EcoRI sites of the pMT/V5 His-B vector (Invitrogen) containing in-frame mTurquoise2 gene at the 5’ end ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Biochemistry 2019Quote: ... the polynucleotides encoding GtACR1 mutants were fused in frame with a C-terminal eight-His tag and subcloned into the pPIC9K vector (Invitrogen) between EcoRI and NotI sites ...
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bioRxiv - Bioengineering 2019Quote: ... precipitate and soluble of was checked for the expression by SDS-PAGE and confirmed indirectly by Western blot with anti-his antibody (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... 1-2 μg His-tagged calcineurin was first bound to Ni-NTA-agarose or magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... mCherry-(backbone pLV-CMV-bc) and FLAG-His-tagged(backbone Pcdna3) p16INK4A constructs were created using gateway technology (Life Technologies). For cell cycle profiling ...
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bioRxiv - Synthetic Biology 2019Quote: ... An anti-6His antibody (6x-His Tag Polyclonal Antibody) and an anti-HA antibody (HA Tag Polyclonal Antibody) were purchased from Invitrogen. Western blot analysis was conducted as described previously 45.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The BglII and EcoRI restriction sites were used to insert the sequence into the pMT/BIP/V5-His plasmid (ThermoFisher). The production of recombinant IrSPI protein was performed by the Recombinant Proteins in Eukaryotic Cells Platform ...
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bioRxiv - Molecular Biology 2019Quote: ... Emulsion PCR was performed using the Ion PGM Hi-Q View OT2 Kit and the Ion OneTouch 2 system (ThermoFisher). Template-positive ion sphere particles (ISPs ...
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bioRxiv - Molecular Biology 2019Quote: ... Enriched ISPs were loaded on a PGM 314R v2 chip and sequenced using the Ion PGM Hi-Q View Sequencing Reagents (ThermoFisher). Raw sequencing data were processed using the Torrent Suite software v5.0 (ThermoFisher) ...
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bioRxiv - Genomics 2021Quote: ... TaTLP2-B gene was re-amplified from the confirmed clone using the same primers and ligated into the pYES2.1/V5-His-Topo vector (Invitrogen, USA). The recombinant plasmid (pYES2.1-TaTLP2-B ...