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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... was used to assess the number of cycles necessary to amplify libraries to the concentration needed for sequencing by amplifying 1 uL of library with LabTAQ Green Hi Rox master mix (Labtech) and adapter-targeted primers on a StepOnePlus Real-Time PCR system (Thermofisher Applied Biosystems). Indexing PCR involved double indexing (Kircher et al ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of His-GLPR at 25 μM was incubated with 20x molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Scientific) following supplier instructions ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Microbiology 2023Quote: ... Extended products were purified by alcohol precipitation followed by dissolving in Hi-Di formamide and analysing in an ABI3700 automated DNA Analyzer (Applied Biosystems). Raw sequences are assumable and aligned using NCBI blast and submitted to NCBI databank for accession numbers ...
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bioRxiv - Immunology 2023Quote: ... monocytes/macrophages (CD3-CD19-NKp46-CD11b+Gr1loLyC6int/hi) and neutrophils (CD3-CD19-NKp46- CD11b+Gr1+LyC6int) were counted using a Countess II Automated Cell Counter (Thermo Fisher) and then combined and processed as one sample per mouse ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were digested with EcoRI and XbaI and inserted into the mammalian expression vector pcDNA-V5-HIS-B (Invitrogen Corp.) that had been cut with the same enzymes ...
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bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... or pCDNA SARS-CoV-2 ORF7a-V5/His (WT/H47Y; 0.75 μg) or empty vector using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μl of each diluted and multiplexed PCR product was then added to 10 μl of Hi-Di Formamide and 0.1 μl of GeneScan 500 LIZ Size Standard (Applied Biosystems Inc.) to prepare for genotyping ...
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bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA fragment encoding the full-length Cckbr1 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The primers used here are listed in Extended Data Table 3 ...
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Cell Biology 2020Quote: The frozen supernatants were used for IL-2 ELISAs according to the manufacturer’s protocol (KMC0021, Thermofisher).
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific, Rockford, IL, USA). Samples were prepared for SDS-PAGE in Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the heart using TRIzol reagent (Thermo Fisher Scientific, Rockford, IL, USA) as reported previously.8 Total RNA was reverse transcribed into cDNA using random primers (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Qubit® Protein Assay Kit (Thermo Scientific Rockford, IL, USA). For both cases ...
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bioRxiv - Neuroscience 2021Quote: ... The protein band was detected using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2021Quote: The LysoTracker Red assay was used to follow the manufacturer’s protocol (LifeTechnology, ThermoFisher, Rockford, IL, USA). LysoTracker Red (50 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% sodium dodecyl sulfate (SDS) in 50 mM triethylammonium bicarbonate (TEAB, Thermo Fisher Scientific, Rockford, IL), pH=8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared in the modified RIPA buffer containing protease inhibitors (Thermo Scientific, Rockford, IL). Equal amounts of proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was estimated by BCA (Pierce BCA Protein Assay Kit, Thermo Scientific, Rockford, IL, USA). Denatured proteins were reduced with 5 mM DTT ...
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bioRxiv - Biochemistry 2019Quote: Primary enriched CM and hiPSC-CM were lysed in 1X Invitrosol (Thermo Fisher Scientific, IL, USA), 100 mM ammonium bicarbonate (NH4HCO3) ...
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bioRxiv - Immunology 2019Quote: ... The protein concentrations were determined by the bicinchoninic acid assay (BCA, Pierce Biotechnology, Thermo Scientific, IL). Further processing was adapted from a filter-aided sample preparation (FASP ...
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bioRxiv - Physiology 2020Quote: ... The PCR amplifications were performed with SYBR green qPCR master mix (#K0252, Thermo Scientific, Rockford, IL). Total RNA was prepared from the frozen whole uterine and placental tissues ...
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bioRxiv - Biophysics 2019Quote: ... proteins were purified using HisPur Cobalt Resin according to the manufacturer’s protocol (Thermo Scientific, Rockford, IL.) and Ulp-1 protease (Ubl-specific protease 1 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher scientific Inc., Rockford, IL). CHT-mediated choline uptake was determined as total choline uptake minus the uptake in the presence of hemicholinium-3 and maximum transporter velocity (Vmax ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected and used for cytokine measurement with IL-18 (Invitrogen; sensitivity 19.0 pg/mL), IL-1β (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: Pierce Bicinchoninic acid (BCA) and MicroBCA protein assay kits were purchased from Thermo Scientific (Rockford, IL). Quant-iT PicoGreen dsDNA assay kit was procured from Molecular Probes ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were transferred onto Biodyne B nylon membranes (0.45 μm; Thermo Fisher Scientific, Rockford, IL, USA) in 0.5X TBE buffer (60V for 30 min) ...
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bioRxiv - Biochemistry 2021Quote: ... that contained 1mg/ml Texas Red-dextran (10,000 MW, Lysine Fixable, Thermo Fisher Scientific, Rockford, IL) at 37°C for 6-24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... M1 tri-Ub was purified to homogeneity by Ni2+-NTA affinity column (Thermo Scientific, Rockford, IL) chromatophraphy ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was determined using the bicinchoninic acid assay (BCA) (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined by the Bicinchoninic acid method (Pierce, ThermoFisher Scientific Fisher, Rockford, IL, USA). Aliquots of lysates containing 30 μg of protein were separated on SDS-PAGE and transferred to Immobilon-P membranes ...
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bioRxiv - Cell Biology 2021Quote: Samples were analyzed on Orbitrap Fusion Lumos Plus mass spectrometers (Thermo Fisher Scientific, Rockford, IL, USA) coupled with an Easy-nLC 1000 nanoflow LC system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with LIVE/DEADTM fixable Aqua dead cell stain (Thermo Fisher Scientific, Rockford, IL) for 30 min at 4oC ...
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bioRxiv - Biochemistry 2022Quote: ... Using Slide –A-Lyzer –Minidialysis units with a 10,000 Dalton MWCO(Thermo Scientific, Rockford, IL, USA) proteins were renatured by dialyzing out urea in a step wise manner from 6 M in steps of two at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... PKCα A (Cat# s11092) or PKCα B (Cat# s11094) silencer®select siRNA (Thermo Scientific, IL) using the lipofectamine RNAiMAX reagent as we described previously (24).
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bioRxiv - Neuroscience 2020Quote: ... RNA quality and concentration were measured using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Chicago, IL). 500-1000 ng RNA per sample was reverse-transcribed using Applied Biosystems high-capacity reverse transcription kit (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... were purchased from Badrilla (Leeds, UK), while RyR2 (1:1000, #MA3-916) was purchased from Thermo Fisher (Rockland, IL, USA). The primary antibodies against p62 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... then protein was quantified using BCA protein assay kit (Thermo Scientific, Rockford IL, USA, Ref. 23227) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2019Quote: ... the peptides were labelled with biotin by using Sulfo-NHS-LC-Biotin (Thermo Scientific, IL, USA). The unreacted Sulfo-NHS-LC-Biotin was quenched by 1 M glycine solution ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...