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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... each protein pellet was digested with 2 µg sequencing grade trypsin (Thermo Scientific, Rockford, IL, USA) overnight at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... cells were seeded into Lab-Tek CC2 treated slide chambers (Nalgene Nunc International, Napeville, IL, USA); after washing with PBS cells were fixed with 4% paraformaldehyde/PBS for 10 min ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Immunology 2019Quote: ... the human IL-2 ELISA kit was used in accordance with manufacturer’s instructions (Thermo Fisher Scientific). Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... and soluble Mpro proteins were purified with an Ni-NTA resin (Thermo-Fisher Scientific, Rockford, IL) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned using CloneJET PCR Cloning Kit (K1231, Thermo Scientific, Fisher Scientific, Hanover Park, IL, USA). Full-length duck ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Immunology 2021Quote: ... TaqMan gene expression kits were utilized to measure target genes of interest: Il-1β (Mm00434228_m1, ThermoFisher), Il-6 (Mm00446190_m1 ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgE Abs were detected with goat anti-mouse IgE-unlabeled (Southern Biotechnology Associates ...
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bioRxiv - Synthetic Biology 2022Quote: ... the yeast genomic DNA was extracted with Yeast DNA Extraction Kit (Thermo Fisher, Rockford, IL, USA). Three primers ...
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bioRxiv - Microbiology 2022Quote: ... before it was developed with SuperSignal West Dura Extended Duration substrate (Thermo Fisher Scientific, IL, USA). The blot was imaged using a Bio-Rad Chemidoc XRS+ (Bio-Rad ...
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bioRxiv - Systems Biology 2022Quote: ... The cell lysates were prepared in RIPA buffer containing protease inhibitors (Thermo Fisher Scientific, Rockford, IL) immediately after treatment ...
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bioRxiv - Neuroscience 2022Quote: ... NPC cultures received 100 pM acetic acid vehicle or 100 ng/ml IL-6 (Gibco; PHC0066) on day 18 for 3h ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were used to perform ELISA to quantify the levels of IL-1β (Thermo Fisher Scientific), IL-6 (Biolegend) ...
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bioRxiv - Genetics 2022Quote: ... cDNA was amplified using High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems, Rockford, IL, USA). The GCLC sequence was amplified using the following primers ...
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bioRxiv - Microbiology 2024Quote: ... Formulations were dialyzed against PBS (pH 7.2) in the dialysis cassettes (Thermo Scientific, Rockford, IL, USA) for at least 18 h ...
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bioRxiv - Cell Biology 2024Quote: ... and activated 1:1 with CD3/CD28 Dynabeads in 100 U/mL of IL-2 (Thermofisher). The next day ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). Amplification was accomplished over 30 cycles as follows ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). The reaction conditions were as follows ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). The reaction conditions were as follows ...
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bioRxiv - Immunology 2024Quote: ... The cells were treated for 18-24 hours with 10 ng/mL IL-1β (Gibco, #PHC0815) and 5 ng/mL TNF (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were harvested for IL-1β measurement using a specific ELISA (Thermo Fisher, 88-7621-22). Apoptotic cells were generated by retroviral transduction or MTX treatment during 20-24 h when cell death approaches its maximum ...
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bioRxiv - Biophysics 2023Quote: ... and then immunoreactive bands were visualized using Super Signal WestPico chemiluminescent substrate (Thermo Scientific, Rockford, IL). Densitometric analysis of nonsaturated films was performed using Bio-Rad Quantity One analysis software (Hercules ...
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bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
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bioRxiv - Molecular Biology 2023Quote: ... or inserts in 24-well plates (0.4 μm polyester membrane, CoStar, Thermo Fisher Scientific, Rockford, IL) until 90% confluence was achieved(102) ...
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bioRxiv - Biochemistry 2023Quote: ... Nuclear fractions were obtained using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, IL, USA).
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bioRxiv - Neuroscience 2023Quote: ... Detection was performed through the chemiluminescence assay with an ECL plus kit (Thermo Scientific, IL, USA). Densitometric detection was carried out with the LI-COR Odyssey imager and Image Studio software (version 5.2 ...
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bioRxiv - Immunology 2023Quote: ... LUVA supernatants were assayed for IL-6 and TNF (Invitrogen, #88-7066-88, #88-7346-88). β-hexosaminidase was measured in fresh supernatants by transferring 20µl sample/well to a 96 well plate (Sarstedt ...
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bioRxiv - Cell Biology 2023Quote: ... and the resultant tryptic peptides were labeled with acetonitrile-dissolved TMT reagents (Thermo Scientific, Rockford, IL), and equal amounts of labeled samples were mixed before prefractionation with reversed phase (RP)-high performance liquid chromatography (HPLC) ...
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bioRxiv - Immunology 2023Quote: ... The gels were fixed and stained with Blue Safe Protein Stain (Thermo Scientific, Rockford, IL, USA) or transferred to a PVDF membrane using a Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... The blots were then developed using the ECL western blotting substrate (Thermo Scientific, Rockford, IL, USA). The levels of protein expression were semi-quantified by optical densitometry using Image J Software version 1.46 (49) ...
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bioRxiv - Pathology 2023Quote: ... It was determined using a Pierce® BCA protein assay kit (Thermo Scientific, Rockford, IL, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and IL-12 was quantified with ELISA kits according to the manufacturer’s instructions (Invitrogen, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 1x protease inhibitor (Pierce Protease Inhibitor Tablet, EDTA-Free, ThermoFisher Scientific, Rockford, IL, USA) and the light units (LU ...
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bioRxiv - Biochemistry 2024Quote: Fisher OptimaTM grade reagents were used for sample preparation and analysis (Fisher Scientific, Hanover Park, IL). EquiSPLASH® LIPIDOMIX (Avanti Polar Lipids Inc. ...
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bioRxiv - Immunology 2024Quote: ... IL-5 levels were quantified in undiluted BAL fluid by ELISA following the manufacturer’s instructions (Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with primary antibodies overnight at 4°C and secondary antibodies conjugated with Alexa 488 (rabbit) and Alexa 555 (mouse) (Life Technologies, Cat. A-21206 and A-31570 respectively) at 1:400 dilution were used ...
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bioRxiv - Physiology 2020Quote: ... βTC3 cells were transfected with plasmids encoding mouse furin and/or mouse insulin receptor (pcDNA3.1 backbone) and/or mouse Atp6ap1/Ac45-Flag using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a, Abcam; Mouse anti-mCherry, Invitrogen) in 0.2% Triton and 5% Goat serum in PBS at 4 °C for 24 -36 hours ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG or anti-mouse IgA conjugated with HRP (Invitrogen) was added ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min and rinsed three times with TBS-T (0.05 % tween-20) ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
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bioRxiv - Immunology 2021Quote: ... 4°C) and cells were spun onto glass slides using a Cytospin 4 Cytocentrifuge (Thermo Scientific), dried for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were migrated on precast Novex 4–20% or 4–12% polyacrylamide gels (Thermo Fisher Scientific), then transferred to Novex nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Neuroscience 2020Quote: ... and then fixed overnight at 4°C in 4% paraformaldehyde (Alfa Aesar, Thermo Fisher Scientific, UK), followed by several washes in 0.1M Phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... only 4 μL of 1 mM FM 4-64 (Cat. number T13320, Thermo Fisher, Waltham, MA) was injected ...