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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
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bioRxiv - Plant Biology 2023Quote: ... These cassettes were sub-cloned into a modified binary cas9 vector pH-Ubi-cas9-7 (Fayos et al., 2020; Miao et al., 2013) in a Gateway LR reaction (Invitrogen) to generate p-OsCPR5.1-A and p-OsCPR5.1-C constructs (Fig ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Cancer Biology 2022Quote: The COS-7 cells were seeded on gold-nanoparticle-coated cover glasses and cultured overnight in DMEM (11965-092, Gibco) containing 10% FBS (10082147 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ABI QuantStudio (model 7 Flex) was used for quantitative PCR reactions with SYBR green master mix (Applied Biosystems; Cat #4367659). The catalog of chosen primers can be found in supplementary data (Supplementary Table 1) ...
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bioRxiv - Biochemistry 2024Quote: ... Liposomes at 5-7 mM were applied to the carbon side of a grid held in a Vitrobot Mark IV (ThermoFisher) chamber kept at 4°C and 100% relative humidity ...
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bioRxiv - Developmental Biology 2024Quote: Animals were kept individually in 6-well plates with 7 mL of 0.22 µm FASW and final concentration of 1,000 U/mL penicillin and streptomycin (Fisher Scientific, 15140122). Feeding began 3 days post amputation to allow for the gut to reform ...
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bioRxiv - Molecular Biology 2024Quote: The fluorescence-microscopy based analysis of proliferation described above for the screen was used to validate the effect of the 7 selected miRNA mimics (Dharmacon, ThermoFisher) on HPASMCs (n=4) ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... RT-qPCR assays were carried out in accordance with the manufacturer’s instructions on the QuantStudioTM 7 Flex RT PCR System (Applied Biosystems), utilizing the indicated amplification cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... or 96-well flat-bottom plates at a concentration of 7 × 105 – 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco). For both workflows ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 58°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). For the telomere primers ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 56°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). Each sample was run in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... was performed using the Applied Biosystems QuantStudio 7 Flex Real-Time PCR system and analysed with the QuantStudio Real-Time PCR Software (v1.7 Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Immunology 2024Quote: ... BHK-21 cells (7×105/well) were transfected in tissue culture-treated 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) with the LCMV Arm plasmids pCAGGS-NP (0.8 µg) ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s directions on a QuantStudio 7 Flex Real-Time PCR System (Cat. No. 4485701, Applied Biosystems, Waltham, MA). Amplification data and melt curves were analyzed using the QuantStudio Real-Time PCR software v1.7.1 ...
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bioRxiv - Immunology 2024Quote: ... The protein was purified with water using a 10 mL 7 kDa MWCO Zeba spin desalting column (Thermo Fisher Scientific), and then by dialysis using a 3.5 kDa MWCO regenerated cellulose Spectra/Por®3 dialysis membrane (Spectrum Labs) ...
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bioRxiv - Neuroscience 2022Quote: ... samples were diluted to 1M urea with a blend of 468 μL of 50 mM ammonium bicarbonate (32) and 7 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25°C for digestion with trypsin ...
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bioRxiv - Immunology 2023Quote: Lungs were transferred into 7 ml bijoux tubes containing 500 µl digestion mix consisting of 5 mg/ml collagenase I (Gibco) and 10 µg/ml DNase I (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR reactions were performed on a QuantStudio 7 Flex PCR machine using PowerUp Sybr Green Master Mix (Applied Biosystems). Corresponding changes in the expression levels of selected genes were calculated using the ΔΔCt method relative to housekeeping genes ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... Coding sequences were amplified using total RNA from 7 days post-fertilization (dpf) zebrafish using the SuperScript IV One-step RT-PCR kit (ThermoFisher) using the primers listed in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were heated 5 min at 95 degrees and analysed on a 7 M urea 15% acrylamide gel further stained with SYBR Gold (ThermoFisher). As a positive control ...
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bioRxiv - Cancer Biology 2023Quote: MCF-7 and MDA-MB 231 (was a gift from Dr Annette Meeson, Newcastle University) were cultured in DMEM (GIBCO) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... iTaq Universal SYBR Green Supermix was used according to the manufacturer’s protocol for RT-qPCR reaction in a QuantStudio 7 Flex (Applied Biosystem by Life Technologies). qRT-PCR primers can be found in Supplementary Information Table1 ...
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bioRxiv - Developmental Biology 2023Quote: ... and Ccnd1 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems), as described above ...
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bioRxiv - Microbiology 2023Quote: ... Glass coverslips with CFBE41o-cells grown for 7 days were affixed into the chamber with continuous flow of Minimal Essential Media (MEM) (GIBCO) without phenol red supplemented with 2 mM L-glutamine (GIBCO) ...
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bioRxiv - Plant Biology 2023Quote: ... 7-day-old seedlings stably expressing LIP1-GFP were vacuum-infiltrated for 30 min with 200 nM MitoTracker Orange (Invitrogen). To test for colocalization seedlings were imaged on a confocal microscope (Zeiss LSM 780 ...
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bioRxiv - Cell Biology 2023Quote: ... the COS-7 cells were transfected with plasmids encoding tubulin (3×mEmerald-ensconsin) and ER (mCherry-KDEL) by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol17 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... quantitative polymerase chain reaction (qPCR) was performed using a QuantStudio 7 Flex qPCR system (4485701; ThermoFisher Scientific, Waltham, MA, USA) and PowerTrack SYBR Green Master Mix (A46109 ...
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bioRxiv - Biophysics 2022Quote: The proteoliposomes were incubated with buffer IN (100 mM KCl, 20 mM HEPES pH 7, containing either 250 μM Calcein (Invitrogen), 100 μM Fura-2 (ThermoFischer Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 5-7 days once they reached about 70-80% confluency using TrypLE cell dissociation reagent (Gibco) and gentle scraping before being replated onto new MEFs ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative Real-Time PCR was performed using KAPA SYBR FAST One-Step kit (KAPA) on a QuantStudio 7 Flex (ThermoFisher) with the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with Annexin V and propidium iodide using Annexin V apoptosis detection kit (Invitrogen Cat# 50-929-7) as per manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with either 0.1% DMSO or 7 nM bortezomib and 30 min prior to fixation cells were treated with MitoTracker Deep Red (500 nM, Invitrogen). 16 % paraformaldehyde (EMS ...
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bioRxiv - Neuroscience 2023Quote: ... 7 x 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM (# 41965062, Thermo Fisher Scientific) containing 10% fetal bovine serum (#16000-044 ...
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bioRxiv - Immunology 2023Quote: ... Bone Marrow Derived Macrophages were isolated and cultured as previously described (Schappe et al., 2018) and differentiated into mature macrophages over 7 days through culture in BMDM media (Gibco RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... Bone Marrow Derived Macrophages were isolated and cultured as previously described (Schappe et al., 2018) and differentiated into mature macrophages over 7 days through culture in BMDM media (Gibco RPMI 1640 ...
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bioRxiv - Physiology 2023Quote: ... qPCR was performed using the QuantStudio™ 7 Flex Real Time PCR System (Applied Biosystems™, New York, NY, USA) and SYBR Green Supermix (Bio-Rad ...