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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 cells were seeded into LabTekII 2-well chambered coverglass (#155380, ThermoFisher Scientific) pre-coated with 0.01% Poly-L-lysine (#P4707 ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Fluorescent dyes including 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, D21490, 2 μg/mL), wheat germ agglutinin (WGA ...
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bioRxiv - Microbiology 2020Quote: ... with 2 Units of S1 enzyme (ThermoFisher, 2 uL of a 1:100 dilution) and 1X S1 Buffer ...
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bioRxiv - Microbiology 2021Quote: ... 2% fetal bovine serum (S182H-500, Eurobio) and N-2 Supplement (17502048, Thermo Fisher) at 37°C and 5% CO2.
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bioRxiv - Microbiology 2022Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS) and 2 µL 20 mg/mL proteinase K (25530049, Thermo Fisher Scientific). Samples were incubated overnight at 65 °C and 800 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl 10% SDS plus 2 µl proteinase K (Thermo Fisher Scientific, Cat# EO0492) were added and incubated for 60 min 37°C ...
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bioRxiv - Cell Biology 2024Quote: Fura-2 acetoxymethyl ester (fura-2/AM) was from Molecular Probes (Leiden, The Netherlands). High-glucose Dulbecco’s modified Eagle’s medium (DMEM) ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/ml of fibroblast growth factor-2 (FGF-2) (all from Gibco). Cells were seeded at a density of 3200 cells/cm2 until they reached 90% confluency ...
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bioRxiv - Neuroscience 2023Quote: ... and 5ng/mL bovine fibroblast growth factor-2 (bFGF-2, #13256-029, Gibco™)) (proliferative conditions) ...
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bioRxiv - Cancer Biology 2023Quote: ... animals were subjected to intracardiac perfusion with 2% PFA-2% glutaraldehyde (A17876, ThermoFisher Scientific) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Genomics 2023Quote: ... and 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Gibco 15630080), and incubated at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... sperm were incubated with 15 μM DEACM-caged cGMP for 7 min in the presence of 0.25% Pluronic™ F-127 (ThermoFisher). Uncaging was performed in the stopped-flow cuvette by a 360 nm LED (Thorlabs ...
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bioRxiv - Biophysics 2021Quote: The proteoliposomes were incubated with buffer IN (100 mM KCl, 20 mM HEPES pH 7, containing either 250 μM Calcein (Invitrogen), 100 μM Fura-2 (ThermoFischer Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... Baculovirus was harvested about 7 days after transfecting bacmid into Sf9 cells cultured in Sf-900 III SFM medium (Gibco) at 27 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR was performed on a high-productivity real-time quantitative PCR ViiATM 7 system (Life Technologies, U.S.A.) using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Cell Biology 2020Quote: ... were injected in the pancreas with 4 ml of collagenase solution (7 ml HBSS, 70 μL HEPES (10 μM) (Gibco), 16,5μL DNAse (10mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative transcript analysis was performed using a QuantStudio 7 Flex Real-Time PCR System with the Power SYBR Green RNA-to-Ct Real-Time qPCR Kit (all ThermoFisher). Total RNA template (25 ng per reaction ...
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bioRxiv - Immunology 2021Quote: ... total bone marrow cells were isolated from the femur and tibias of mice and differentiated into macrophages for 7 days in complete DMEM medium (500 mL, ThermoFisher) supplemented with M-CSF (40 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from pygidia and several posterior segments of young regenerates (7-10 days of regeneration) of Alitta virens and Platynereis dumerilii using TRIzol reagent (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng template was used per reaction with SYBR Green Mastermix in ViiA 7 Real-Time PCR System (Applied Biosystems). As endogenous control ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was then performed for at least 3 biological replicates using Biozym Blue S’Green qPCR Mix Separate ROX (No. 331416XL) on QuantStudio 7 Flex Real-Time PCR System from Applied Biosystems. Pax3 fold change was calculated from ΔCt using Gapdh as housekeeping gene (2^-(ΔCt)) ...
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bioRxiv - Developmental Biology 2021Quote: Micropatterned coverslips with 225-μm diameter synthetic lung buds at day 7 of lung induction were dissociated with TrypLE Express (Gibco) for 10 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... was cultured in Dulbecco’ ss Modified Eagle Medium (DMEM) supplemented with 7% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco) at 37°C with 5% CO2.
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bioRxiv - Immunology 2022Quote: ... was used and results were analyzed by quantitative RT-PCR with a Vii 7 Real-time PCR system (Applied Biosystems). The comparative threshold cycle method and internal control (Gapdh ...
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bioRxiv - Developmental Biology 2020Quote: ... We use the following conditions for the 40-cycle reaction on the Quant Studio TM 7 Flex System (Thermo Fisher): a ...
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bioRxiv - Microbiology 2019Quote: ... Samples with RIN > 7 and DNA contamination <10% were used for cDNA synthesis using SuperScript III first-strand synthesis kit (Invitrogen). RT-qPCR was performed using 2xSYBR FAST qPCR universal MasterMix kit (Kappa Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Developmental Biology 2019Quote: ... After 7-10d culture cells were shaken in an orbital shaker at 350-400rpm (1.9cm orbital radius, MaxQ 4450, ThermoFisher Scientific) at 37°C to obtain a culture of cortical astrocytes ...
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bioRxiv - Biophysics 2019Quote: 20-nm fluorescent dark red beads samples (Fig. 2d, Supplementary Fig. 5) were prepared using a 5.10−7 dilution of the initial solution (F8783, Thermo Fisher). We performed the dilution in PBS + 5% glucose to match the index of the dSTORM imaging buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF-7 and MDA-MB-231 cells were plated on glass coverslips in serum-free Gibco OptiMEM medium (Life Technologies) and transduced with 4.8×1010 PFU of Ad5-Laconic (Vector Biolabs) ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... of WT and Adrb2-/- Cl4 mice (7-12 weeks old) with a negative isolation kit according to the manufacturer’s instructions (Invitrogen #11417D). Donor T cells were derived from CD90.1 congenic animals in order to track their frequency in CD90.2 hosts ...
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bioRxiv - Neuroscience 2020Quote: ... Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at −80 °C until sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Biochemistry 2021Quote: ... cell pellets were incubated with 300 µL of 10 mg/mL lysozyme (prepared fresh in sterile H2O) and incubated for 7 minutes at 30°C/250 rpm to allow for efficient lysis by TRIzol reagent (Ambion). 10-15 glass beads (4mm diameter ...
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bioRxiv - Bioengineering 2021Quote: ... All proteins were purified with desalting columns (Zeba Spin Desalting Columns, 0.5 mL with 7 kDa MWCO, Thermo Fisher Scientific) by washing with PBS three times (centrifuging 1500 rcf ...
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bioRxiv - Bioengineering 2020Quote: ... A biphasic solvent overlay was employed to capture patchoulol by adding 7 mL of filter-sterilized n-dodecane (Acros Organics) to each culture (10% v/v) ...
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bioRxiv - Bioengineering 2020Quote: ... The samples were sectioned using Kawamoto’s cryofilm type 2C (SECTION-LAB Co. Ltd., Japan) to 7 µm thickness using a cryotome (CryoStar NX70, Thermo Scientific) according to the Kawamoto protocol [48] ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from HTM cells ± DEX at 7 d (N = 3 per group and donor) using PureLink RNA Mini Kit (Invitrogen). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Biochemistry 2020Quote: MCF-7 cells (150–1000 cells/well) were plated in Labtek Permanox Plastic Chamber slide system (Nunc, Thermo; cat. #177445) and incubated overnight ...