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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... and incubated in cell* dishes at 37° C for 4 – 7 hours before adding cell culture medium (RPMI 1640, -glucose +glutamine, Gibco), supplemented with penstrep (10,000 U/ml penicillin / 10,000 μg/ml streptomycin ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... 18.7 mM NH4Cl and 0.2 g/L glucose and supplemented with 20 mL/L MEM essential amino acids (Gibco 11130051), 10 mL/L MEM nonessential amino acids (Gibco 11140050) ...
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bioRxiv - Genomics 2020Quote: ... Quantitative transcript analysis was performed using a QuantStudio 7 Flex Real-Time PCR System with the Power SYBR Green RNA-to-Ct Real-Time qPCR Kit (all ThermoFisher). Total RNA template (25 ng per reaction ...
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bioRxiv - Pathology 2021Quote: ... Quantitative miR expression data were acquired and analyzed using the ViiA 7 real-time PCR system (Applied Biosystems, Cat# 4453545).
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bioRxiv - Pathology 2021Quote: ... and 001234) and ViiA 7 real-time PCR system (Cat# 4453545) were purchased from Applied Biosystems (Foster City, CA, USA). Rabbit polyclonal anti-calnexin (CNX ...
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bioRxiv - Molecular Biology 2021Quote: ... using a 7900HT Fast Real-Time PCR System or a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). The mRNA expression levels were calculated with the 2-ΔΔCt method ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time PCR was performed and analysed using the ABI ViiA™ 7 real time PCR system (Thermo Fisher Scientific). TaqMan Gene Expression Assays (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... African Green monkey kidney epithelial cells) and Huh-7 cells (human hepatocellular carcinoma cells) were cultured in Dulbecco’s minimal essential medium (DMEM; Life Technologies), supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... Digestion was stopped by addition of an 8.6% SDS/7 mM EDTA solution and crosslinks were reversed by adding Proteinase K (20 mg/ml; Life Technologies) and incubating 6 hr at 65°C ...
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bioRxiv - Cell Biology 2019Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (AL007A, HiMedia, Mumbai, Maharashtra, India) supplemented with 10% FBS (10270-106, GIBCO-Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and from day 7 cells were fed with fresh RPMI medium supplemented with B27 with insulin (#17504044, Thermo Fisher Scientific) without extra supplement ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 18.7 mM NH4Cl and 0.2 g/L glucose and supplemented with 20 mL/L MEM essential amino acids (Gibco 11130051), 10 mL/L MEM nonessential amino acids (Gibco 11140050) ...
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bioRxiv - Cell Biology 2020Quote: ... FACS analysis of transfected BEAS-2B cells for caspase 3 & caspase 7 along with SYTOX AADvancedTM Dead Cell Stain was performed per manufacturer’s protocol (Thermo Scientific).
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bioRxiv - Plant Biology 2021Quote: ... primed reverse transcription of first-strand cDNA synthesis was carried out with 7 mg total RNA using Super ScriptTM III (Invitrogen). Equal volumes of each first-strand reaction were amplified with gene-specific primer pairs ...
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bioRxiv - Biochemistry 2021Quote: 3 biological replicates of wild type and 2 different clones METTL5-KO HeLa cells (clone 1 and 7) were grown to confluency prior to lysis in Trizol (Invitrogen). Total RNA was purified as described above (‘RNA purification’ ...
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bioRxiv - Physiology 2021Quote: ... JC-1 dye was added 10 min prior to recording (ex.488/em.535 and em.590) while CellEvent Caspase 3/7 (ThermoFisher) was added immediately prior to recording (ex.495/em.540).
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bioRxiv - Microbiology 2019Quote: ... was used to quantify the relative levels of cells using a Viia™7 Real-Time PCR Instrument (Applied Biosystems) with 50 ng gDNA template using the following primers ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane and analyzed by western blotting using an anti-His tag antibody (7) or stained with Krypton fluorescent dye (ThermoFisher) according to a company’s instruction ...
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bioRxiv - Bioengineering 2021Quote: Spleens and injection dLNs were collected on day 28 (7 days post-boost) and stored in ice-cold IMDM (Gibco) until further steps ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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bioRxiv - Bioengineering 2021Quote: ... Culture medium from day 0 until day 7 was RPMI1640 (HyClone, SH30027.01) plus B-27 serum-free supplement without insulin (Gibco, A1895601). From day 7 and onwards RPMI1640 with B-27 serum free supplement with insulin (Gibco ...
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bioRxiv - Pathology 2021Quote: ... 35 mins in MES buffer for aSyn detection) and transferred to nitrocellulose membranes (0.2 μm) via Iblot (7 mins, 20 mV; Fisher Scientific). Initial blots were tested for optimisations of aSyn retention as labelled by MJFR1 either by pre-blocking heat treatment (microwaved ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml -CD28 and 1:50 diluted conditioned supernatant from COS-7 cells transfected with a pcDNA1 vector (Invitrogen) encoding for recombinant chicken IL-2 (a kind gift from prof ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated with 7 µl of resuspended RNA using SuperScript™ III First-strand synthesis system (Thermo Fisher Scientific), and primers A1 and A2 (Table 1 ...
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bioRxiv - Immunology 2020Quote: Cell survival was determined by analyzing forward/sideward scatter properties of cells and simultaneous staining with either Propidium iodide (PI), or 7-amino-Actinomycin D (7AAD eFluor670 dye, and LIVE/DEAD fixable violet stain (ThermoFisher), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of these genes was performed using SYBR Green on a ViiA™ 7 sequence detection system (Applied Biosystems, USA) with two replications for each sample ...
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 2019-nCoV CDC RUO Kit (Integrated DNA Technologies) and QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). PCR amplicons were quantified against in vitro transcribed RNA N gene fragment standard ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: ... Apoptosis staining was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific, Waltham, MA). Stained cell suspensions were measured with the BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and second strand synthesis performed on the slide with cDNA quantification undertaken with qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA systems) and analysed on the QuantStudio 7-Flex system (ThermoFisher). FFPE samples were cut at the standardised 5 µm thickness onto four 6.5mm x 6.5mm capture areas ...
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bioRxiv - Immunology 2022Quote: ... The amplification reaction was monitored on the ViiA 7 Real-Time PCR System running on the QuantStudio Real Time PCR Software V1.3 (Thermo Fisher). The size of the respective amplicons was confirmed by 2% agarose gel and ethidium bromide staining ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and COS-7 cells were maintained at 37 ºC and 5% CO2 and cultured in Dulbecco’s modified Eagle media (Life Technologies) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Cell Biology 2022Quote: Human breast cancer (MCF-7) cells were obtained from ATCC and cultured every two days in Minimum Essential Medium (MEM) Alpha media (Gibco) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Genetics 2022Quote: ... The medium was changed to 500 μl EDM media 7 days later in 24-well flat-bottom plates for further differentiation (Nunc). After 4-7 days of culturing ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were generated for 7 days in respectively IMDM or RPMI-1640 medium (Gibco), supplemented with glutamine ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Plant Biology 2022Quote: ... Entry clones were then introduced into the destination vector pH-Ubi-Cas9-7 which also encodes the Cas9-enzyme (Miao et al., 2013) using LR recombination (Invitrogen). T1 CRISPR/Cas9 lines were genotyped for the absence of the T-DNA construct by using the primers priMR410/411 (Hyg-1F/Hyg-1R) ...
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bioRxiv - Microbiology 2022Quote: ... TcdB protein was diluted to 0.05 µg/µL using phosphate buffer (100 mM KPO4, 150 mM NaCl, pH 7) containing 5X SYPRO Orange (Invitrogen S6650), and a serial dilution of test compound ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... using either a QuantStudio™ 7 Flex Real-Time PCR System or a StepOnePlus Real-Time PCR System (Applied Biosystems). Individual standard curves were generated specifically for each system ...
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bioRxiv - Microbiology 2022Quote: ... Sydney Strain SS1 (cagA+, vacA s2m2) were grown on brain heart infusion (BHI) agar (Difco, USA) containing 7% heat-inactivated horse serum (Invitrogen), antibiotics and IsoVitaleX as mentioned previously (33) ...
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bioRxiv - Microbiology 2022Quote: ... The loaded plate was amplified for 40 cycles via the ViiA 7 real-time PCR system (Thermo Fisher Scientific, Waltham). For each biological replicates (n = 3) ...