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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Fractions containing purified protein were pooled and dialyzed overnight against storage buffer (0.02 M sodium phosphate, pH 7) in a 10 kD cut off dialysis cassette (ThermoFisher, 66380). Purified protein was stored at 4 °C for use within days or at −20 °C for longer-term storage.
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bioRxiv - Cancer Biology 2020Quote: ... and the doxycycline-inducible COV434 pIND20 BRG1-2.7 and TOV21G pIND20 ARID1A (generously donated by Dr. Bernard Weissman, University of North Carolina) were cultured in DMEM (Gibco) and supplemented with 10% TET free FBS (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... Eyes were first incubated in 1 mL of high glucose Hanks balanced salt solution/0.5 mg/ml proteinase K (37°C, 7 min) and then in Neurobasal medium (Life Technologies) plus 10% fetal calf serum to stop enzymatic activity ...
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bioRxiv - Plant Biology 2021Quote: ... pistils and young seeds (7 DAP from CT and 5 DAP from HT) were isolated following a protocol for Trizol (Invitrogen). RNA from 26 DAP seeds was isolated using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2020Quote: ... qPCRs were carried out on an Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Applied Biosystems, Foster City, California, USA) using isolated genomic DNA as template ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cultures were grown at 37°C either in M9 media [M9 salts (248510, Difco)] supplemented with 0.4% glucose (6363-53-7, Fisher Scientific), 1 mM MgSO4 (Sigma Aldrich) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Microbiology 2021Quote: ... and qPCR were performed on 5µL of the cDNA libraries diluted at 1:10 using the Precision Plus 2x qPCR Mastermix (Primer Design) according to the manufacturer’s instructions and the Quant Studio 7 Flex (Applied Biosystems). For validation of the translatomic results ...
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bioRxiv - Cell Biology 2020Quote: Cos-7 cells were maintained in DMEM + 10% fetal bovine serum and all transfections were conducted using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR was performed using 384-well plates (4titude Ltd, UK) in the ViiA™ 7 System (Applied Biosystems, UK) with the SensiFAST™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... Strip tubes were loaded onto the Genie-II or Genie-III (OptiGene Ltd) and 96-well plates run on a QuantStudio 7 thermocycler (ThermoFisher). Reactions were incubated at 65°C for 30 min with fluorescence acquisition every 30 sec (Genie instruments ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions with RNase T2 were done at room temperature for 10 minutes in 1× Structure Buffer (10 mM Tris pH 7, 100 mM KCl and 10 mM MgCl2; Ambion). Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x 107 MRC5 cells were infected as described above and total RNA was isolated from cells using TRIzol (Invitrogen), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell death was assessed by measuring caspase 3 cleavage using a CellEvent Caspase3/7 Green Flow Cytometry kit (Thermo Fisher), according to the manufacturer’s descriptions ...
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bioRxiv - Cancer Biology 2020Quote: ... was mixed with Taqman assays and analysed in a 384 well QuantStudio Flex 7 (Applied Biosystems Life Tech, Thermo Scientific) in 5μl reaction volumes ...
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bioRxiv - Cancer Biology 2020Quote: ... was mixed with Taqman assays and analysed in a 384 well QuantStudio Flex 7 (Applied Biosystems Life Tech, Thermo Scientific) in 5μl reaction volumes ...
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bioRxiv - Cell Biology 2021Quote: ... The quantitative PCR (Q-PCR) reactions were conducted in triplicate on a ViiA 7 Real-Time PCR system (Thermo Fisher) from 1 μL of cDNA using the PerfeCTa SYBR Green SuperMix reagent (Quantabio Cat# ...
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bioRxiv - Cell Biology 2021Quote: ... The tissues were then digested in room temperature for 7 min in a 3-mL Hank’s solution containing 0.25% Trypsin (Thermo Fisher, 15090046) and 0.1 μg/μL DNase (Sigma D5025 ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher Scientific, 89960; IL). The original murine anti-PD1 monoclonal antibody (63 ...
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bioRxiv - Genetics 2022Quote: ... The volume was filled up to 7 ml with water and 50 units of T4 DNA ligase (Thermo Fisher Scientific) were added ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... and qPCR was performed on 5μL of the cDNA libraries diluted at 1:5 using the Precision Plus 2x 598 qPCR Mastermix (Primer Design) according to the manufacturer’s instructions and the Quant Studio 7 Flex (Applied Biosystems).
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bioRxiv - Immunology 2022Quote: pDC generation: bone marrow cells were isolated (see above) and cultured for 7 days in RPMI-1640 GlutaMAX-I (GIBCO) supplemented with 10% FCS (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 350ng of pCAGIG-VENUS was used to transfect the neurons two days after adding mouse glia (PNI day 7) using Lipofectamine 2000 (ThermoFisher). A full media change using conditioned media was performed 5-6 hours post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Neuroscience 2022Quote: ... neural progenitor cells were manually isolated from rosettes and expanded as neurospheres in a suspension culture for 7 days in E6N2 supplemented with 50x B27 lacking retinoic acid (Invitrogen) and 20 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Neuroscience 2022Quote: ... jGCaMP7b-XC or jGCaMP7b-XN and 1 μg cDNA encoding CFP (for labelling the soma area and neurites) were transiently transfected into DIV 5-7 cultured cortical neurons by Lipofectamine 2000 (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Microbiology 2022Quote: ... as per manufacturer’s instructions in 96-well plates and conducted in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Primers were designed using Primer3 94 to target the hucL gene (HucL_fw ...
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bioRxiv - Biophysics 2022Quote: ... The diluted sample was then passed through a Zeba Spin Desalting Column (7 kDa cut-off, Thermo Fisher Scientific, France) equilibrated in acquisition buffer containing 0.005% LMNG (w/v) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted from rats aged postnatal day 5-7 and placed in working buffer (Hank’s balanced salt solution (HBSS) (Life Technologies) with 0.5% chromatographically purified bovine serum albumin (BSA ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
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bioRxiv - Microbiology 2021Quote: ... The number of PCR cycles for sufficient DNA for downstream applications was empirically determined by quantitative PCR (ViiA 7 Real-Time PCR System, ThermoFisher) in the presence of 1X Evagreen (20X Evagreen ...
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bioRxiv - Cancer Biology 2020Quote: ... Reactions were performed using the pre-set program of the ABI ViiA 7 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... marrow was extracted from femurs and tibias of 8 to 12 week-old male and female mice and differentiated for 7 days into BMM in Dulbecco’s Modified Eagle’s Medium with glutamine (DMEM, Thermo Fisher Scientific), containing 10% heat-inactivated ...
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bioRxiv - Microbiology 2022Quote: ... targeting a region in the viral ORF1ab gene and using a QuantStudio™ 7 Real-Time PCR System (Applied Biosystems). Data were processed using GraphPad Prism software (V8.0 ...
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bioRxiv - Genetics 2022Quote: ... were combined and concentrated to ∼1 mL and dialyzed using 7 KMWCO Snake Skin dialysis tubing (Thermo Scientific, Waltham, Massachusetts) into 20 mM Tris 150 mM NaCl pH 8.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time quantitative PCR was performed on a ViiA 7 Real-Time PCR System with OptiFlex Optics System (Applied Biosystems) using PowerUp SYBR Green PCR kit (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed using SYBR green reagents on an Applied Biosystems QuantStudio 3 and ViiA 7 Real-Time PCR Systems (ThermoFisher).
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bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
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bioRxiv - Molecular Biology 2019Quote: ... 250 ng of m1A-containing synthetic oligonucleotide (see Extended Data Table 7) were spotted in triplicate on a BrightStar membrane (ThermoFisher), allowed to briefly air-dry ...
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bioRxiv - Pathology 2020Quote: ... Gene expression by qPCR was performed using a ViiA™ 7 Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). All reactions were performed as biological triplicates and technical duplicates using a GoTaq® qPCR Master Mix Kit (Promega) ...
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bioRxiv - Biochemistry 2019Quote: ... RNAs after hDcp2 treatment were resolved electrophoretically on denaturing 15% acrylamide / 7 M urea 1xTBE gel stained with SYBR Gold (Invitrogen) and visualized using a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... LC-MS/MS data was collected as previously described.7 The raw data files were processed and quantified using Proteome Discoverer software v1.4 (Thermo Scientific) and searched against bacterial genome and horizontally acquired resistance genes as described previously.8
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bioRxiv - Systems Biology 2019Quote: All the reactions were performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems by Life Technologies). Cycling conditions were ...
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bioRxiv - Systems Biology 2019Quote: All the reactions were performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems by Life Technologies). Cycling conditions were ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed on an ABI PRISM 7900HT or QuantStudio 7 Flex Real-Time PCR System (Life Technologies) with Power SYBR Green PCR Mix (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... To measure leukocytosis blood was harvested 7 days post-infection by cardiac puncture into ethylenediaminetetraacetic acid (EDTA) containing 1.7mL tubes (Fisher Scientific) and treated with ammonium-chloride-potassium (ACK ...
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bioRxiv - Physiology 2019Quote: ... Quantitative Real-time PCR (qRT-PCR) was performed in duplicate using the ViiA 7 Real-time PCR system (Applied biosystems). All expression data were normalized by the 2(-ΔCt ...
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bioRxiv - Microbiology 2020Quote: ... The dose-response plates are 200 times diluted in 300μL Phosphate Buffered Saline (PBS; pH= 7-7.2 without Ca/Mg) (Gibco, 14190-144) by transferring 1.5 μL of serially diluted compound ...