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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... were seeded in a 6-well plate at a cell density of 200,000/cm2 in Dulbecco’s Modified Eagle Media (DMEM, Thermo Fisher) supplemented with 15% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2022Quote: ... All samples were prepared in triplicate and run on a QuantStudio 6/7 Flex Real-Time PCR System (ThermoFisher). Primer sequences for designated HHV-6 marker (U31 ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was resuspended in 1 ml of DMEM before adding the mixture to a 6-well plate containing 2 ml of DMEM with 4.5 g/L D-Glucose and L-Glutamine (Gibco) supplemented with supplemented 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: Cultured N2A control and Fmr1 CRISPR/Cas9 KO cells were seeded in 6-well plates overnight and then transfected using 7.5 µl of Lipofectamine 3000 (Invitrogen) and 5 µl of P3000 with the 1 µg of pFlareA-Mapt exon4 splicing reporter ...
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bioRxiv - Bioengineering 2023Quote: ... and liquid agarose (2.5mL) was used to cover the bottom of a high-performance #1.5 6-well plate (Fisher Scientific). This process was repeated for all five wells ...
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bioRxiv - Genomics 2023Quote: ... and 2µL of primers at 3µM in a total reaction volume of 10µL on qPCR was realized on a QuantStudio 6 Flex Real-Time PCR system (ThermoFisher). Each sample was tested in duplicate ...
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bioRxiv - Neuroscience 2023Quote: ... tissue fragments weighing 200 mg were placed in a 6-well plate containing 2 ml of Hibernate E (Gibco) in each well and were carefully dissociated until all the pieces were the same size ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with 1µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Following 6 days of expansion the filtered cell culture supernatant was applied to a 5ml Ni-NTA column (ThermoFisher) prewashed with 100ml washing solution (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-23,36-39 (Supplementary Methods Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-59 (Supplementary Methods Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells from each embryo were then plated onto a 6-cm culture dish coated with 0.1% gelatin and cultured in DMEM (Hyclone) supplemented with 10% FBS (GIBCO), 1% GlutaMAX (GIBCO) ...
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bioRxiv - Cancer Biology 2023Quote: ... normalized to the expression of GAPDH on a QuantStudio 6 and 7 Pro real-time PCR system (Applied Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... Thermo Scientific’s SYBR green Real-time PCR master mix was used to perform qRT-PCR (Quantstudio 6, ThermoFisher Scientific) in triplicates to determine the fusion gene expression levels using PRCC-TFE3 fusion gene primers (FP:5’-CCAAGCCAAAGAAGAGGAAA-3’ and RP:5’- CGAGTGTGGTGGACAGGTACT-3’).
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant containing secreted pro-HlyA protein was collected by centrifugation (Thermo Scientific, Sorvall RC 6+, SLA-3000 (Rotor), 10,000 × g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression was determined by quantitative real-time PCR using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative gene expression was determined using ΔΔCT method ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293A cells were initially plated at 750k cells/well in 6-well plates in Dulbecco’s modified eagle media (DMEM) supplemented with 10% fetal bovine serum (FBS) and GlutaMax (Gibco) with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 6 µg of PEI were diluted in 0.5 ml of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were assembled for 20 min at room temperature and separated on 6% DNA retardation gels (Thermo Fisher scientific) at 200 V for 1–2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg FLAG-AGAL construct and 4.5 µg pOG44 was transfected into HFTs seeded onto a 6-well plate using Lipofectamine 2000 (Invitrogen). After 48 hours the transfected cells were selected with hygromycin (200 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... supplied with the primers for ICP27 and β-actin on a Quant Studio 6 Flex qPCR system (Applied Biosystems).
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bioRxiv - Developmental Biology 2023Quote: ... Images were collected manually or automatically with 4-6 µs dwell time using Maps 3.11 software (Thermo Fisher Scientific) (Burel et al. ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were mounted with Fluoromount G™ containing 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI, Invitrogen) for nuclei detection ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... resuspended in mounting media containing 4’,6’-diamidino-2-phenylindole (DAPI) (Fluoromount-G™ Mounting Medium, with DAPI, Invitrogen), incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and SEC using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded at a concentration of 150,000 cells per well of a Matrigel-coated 6-well plate in E8 medium (ThermoFisher) containing Rock-inhibitor (10 µM ...
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bioRxiv - Bioengineering 2023Quote: ... We activate the gel surface by sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino) (sulfo-SANPAH, Thermo Scientific, Cat. No. 22589), which allows covalent conjugation of type IV collagen to the gel surface ...
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bioRxiv - Cell Biology 2023Quote: ... and plaque-derived sEVs (n=6) were mixed with NuPAGE lithium dodecyl sulfate sample buffer (NP0007, Thermo Fisher Scientific) at a 4:1 ratio and NuPAGE sample reducing agent (NP0009 ...
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bioRxiv - Genetics 2023Quote: 3 independent total RNA extractions from 30 ovaries from 3-6-day-old RevI-H2i2 flies using Trizol (Invitrogen) were performed ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with secondary antibodies for 2 hours at RT in a humid chamber and DNA was counterstained with 0.1 µg/ml of DAPI (DNA intercalator, 4’,6-Diamidino-2-Phenylindole, D1306, Invitrogen). Coverslips were mounted with ProLong Diamond Antifade (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with SSCT and incubated with 600 nM 4′,6-Diamidin-2-phenylindol (Dapi, D1306, Thermo Scientific) for 10 minutes before final washing in SSCT ...
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bioRxiv - Pathology 2023Quote: ... Cells were spun down at 350g for 6 minutes and were re-suspended with 4mL of 0.05% Trypsin (ThermoFisher) and incubated at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The RT-qPCR was performed using the Promega® 2x Master Mix and the QuantStudio™ 6 (Applied Biosystems) for 40 cycles ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from mating wild-type and EMA2 KO cells were isolated at 6 hpm using TRIzol reagent (Invitrogen). The RNA was treated with Turbo DNase (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... was transduced by lentivirus and successfully transduced cells were selected by 6 μg/ml blasticidin (R21001; Thermo Fisher Scientific) for 1-2 weeks ...
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bioRxiv - Neuroscience 2023Quote: ... Organ of Corti explants were isolated from C57BL/6 mice at postnatal day 4 in cold Leibovitz’s L-15 medium (cat. # 21083027, Gibco) and held in place by two glass fibers glued to the bottom of a Petri dish ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from samples containing 6 OD600 units of mid-log phase cells using Trizol (Invitrogen #15596026) and chloroform ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR (qPCR) was conducted on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, CA, USA). Relative mRNA levels were determined using the Comparative Ct (△△CT ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Genomics 2024Quote: ... and the relative expression was determined by the 2-ΔΔCt method on a QuantStudio 6 qPCR machine (Thermo Fisher). The levels of mRNAs were normalized to human ACTB.
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bioRxiv - Neuroscience 2024Quote: Brains were dissected from 2-day old adults in PBS and incubated in 1.6 × 10-6 M acridine orange (ThermoFisher) for 5 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... equal amounts of RNA were prepared in equal volumes (6 µL) using UltraPure DNase/RNase-free distilled water (Invitrogen). RNA samples were denatured at 95 °C for 3 min and placed on ice immediately to prevent the formation of secondary structures ...