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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... human embryonic kidney 293 (HEK 293)(ATCC) cells were seeded in a 6-well plate at 0.3×106 cells/well in DMEM (ThermoFisher) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2020Quote: ... whereby the transfected population is selected for removal of HXGPRT and replacement with the complementation allele in 6-thioxanthine selection medium [177 µg/mL of 6-thioxanthine (TRC, cat# T385800) in 4.5 g/liter D-glucose in GlutaMAX DMEM (Gibco), with 1% dialyzed FBS (Omega Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection of HCT116 cells (0.6×106 cells/well in 6-well plate) with siRNA oligonucleotides was performed using Lipofectamine RNAiMAX (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) mock- or virus-infected (MOI of 0.01) were extracted using TRIzol reagent (Thermo Fisher Scientific). Superscript® II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were plated at a density of 6 ×105 cells/cm2 and cultured in Neurobasal media (Invitrogen, Carlsbad, CA) supplemented with B-27 (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were mounted on glass slides in 4′,6-diamidino-2-phenylindole (DAPI) containing Fluoromount-G medium (Thermo Fisher). Images were acquired by an Eclipse A1 laser-scanning microscope ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Zoology 2021Quote: ... and the cDNA was used for qPCR analysis using the QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems) and SYBR Green detection system (TOYOBO ...
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bioRxiv - Immunology 2020Quote: ... male C57BL/6 recipient mice were first primed intraperitoneally (i.p.) with 50 mg ovalbumin (OVA) in alum (Thermo Scientific) at 2:1 v:v ratio in 100 μL total volume ...
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bioRxiv - Bioengineering 2020Quote: We used a mixture of 6 μm and 2 μm diameter fluorescence beads (C16509, Thermo Fisher; F8825 Thermo Fisher) in our experiment ...
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bioRxiv - Bioengineering 2020Quote: We used a mixture of 6 μm and 2 μm diameter fluorescence beads (C16509, Thermo Fisher; F8825 Thermo Fisher) in our experiment ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with transfection complexes for 5-6 hours and then replated onto #1.5 coverslips (Warner Instruments) coated with Collagen IV (Gibco). Cells were imaged in DMEM/10% FBS ...
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bioRxiv - Genetics 2021Quote: ... Cells were plated at 0.3-0.4 × 106 in 1.5 ml media in 6-well plates-24 hours before transfection with either rCro or lipofectamine 3000 (ThermoFisher) or Lipofectin (99-102 ...
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bioRxiv - Immunology 2020Quote: ... animals previously injected with vaccine or PBS where injected 24 hrs before sacrifice in the same footpad with 30 μL of 0.5 mM 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen). For assessing migration after 24 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen) and images were acquired using Nikon A1-R confocal microscope using the NIS Elements software ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells were plated at a density of 0.6×106 cells in a 6-well culture dish containing DMEM medium (Gibco), and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR was performed in a 384-well format on a QuantStudio 6-flex Instrument (Applied Biosystems) using PowerUp™ SYBR™ Green Master Mix (#A25780 ...
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bioRxiv - Developmental Biology 2020Quote: ... qPCR reactions were performed in duplicate on an Applied Biosystems Quantstudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) using 4 ng cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were washed in 0.1% IGEPAL/PBS and mounted using ProLong Gold antifade reagent with 4’-6-Diamidino-2-phenylindole (DAPI) (Invitrogen). Immunofluorescence studies in HeLa cells were carried out as described above ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Cell Biology 2019Quote: ... Mounting medium contained DAPI (4’, 6-diamidino-2-phenylindole) to visualize the nucleus (Molecular Probes Inc., Eugene, OR, USA). Negative controls were performed with either no primary or no secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... incubated 5 minutes with at room temperature with 500 ng/ml 4’,6-Diamidino-2-Phenylindole Dilactate (DAPI; ThermoFisher) in DPBS and mounted in ProLong®Gold (ThermoScientific).
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bioRxiv - Physiology 2021Quote: ... Cells were treated with dexamethasone (1μM) or vehicle (0.01% ethanol) and lysed 6 or 24 hours later by adding 0.5ml TRIzol (Invitrogen, ThermoFisher, UK) following removal of medium ...
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bioRxiv - Physiology 2021Quote: ... Cells were treated with dexamethasone (1μM) or vehicle (0.01% ethanol) and lysed 6 or 24 hours later by adding 0.5ml TRIzol (Invitrogen, ThermoFisher, UK) following removal of medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat-induced antigen retrieval was performed by incubating slides in a solution of 10 mM sodium citrate pH 6 (MilliporeSigma; SX0445-20) with 0.05% (v/v) Tween20 (ThermoFisher) at 98C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... For siRNA experimentsHEK293T cells (1.5×105 cells/ml) were seeded in 6-well plates and transfected with 150nM siRNA using 4μl Lipofectamine 2000 (Invitrogen) and 184μl OptiMEM ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids expressing TSC22D4 alleles and Akt1 were transiently co-transfected to Hepa1-6 cells using Lipofectamine 2000 (Invitrogen, #11668019) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... newly formed clusters were transferred into Low-attachment 6-well plates (Cat. No. 07-200-601, Thermo Fisher Scientific) and cultured with RPMI 1640 plus GlutaMAX + 1% (v/v ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Cell Biology 2021Quote: ... For transfection, 375 ng of synthetic sgRNA (TrueGuide™, CRISPR932782_SGM) along with 6 μL Cas9 mRNA (Invitrogen™, A29378), and 18 μL of the MessengerMAX™ reagent (Invitrogen™ ...
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bioRxiv - Cell Biology 2019Quote: ... at a ratio of 1 μg eYFP-PCNA to 0.1 μg hygromycin marker to 6 μl FuGENE6 according to the manufacturer’s instructions except that we washed cells with Opti-MEM (Invitrogen), transfected cells in Opti-MEM ...
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bioRxiv - Genetics 2021Quote: ... and Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) plus GlutaMax (Thermo Fisher), supplemented with 10% (v/v ...
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bioRxiv - Genomics 2019Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (4.4 g/L MS salts mixture, 10 g/L D-sucrose) in 6 well-plates (Nunc) and allowed to acclimate for 48 hours with mild agitation (130 rpm).
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bioRxiv - Microbiology 2021Quote: ... MDCK cells were grown to 90-95% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 10% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Microbiology 2020Quote: 4 μl of purified spores (6×107 spores/ml) were mixed with 40 μl of NucBlue (Invitrogen, catalog #R37605) and incubated at 25°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: Bone marrow derived macrophages (BMDMs) were generated from 6-8 week old BALB/c mice in RPMI-1640 (Gibco) containing 10% foetal calf serum ...
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bioRxiv - Microbiology 2021Quote: ... were used to amplify cellular RNA and viral RNA by QuantStudio 6 Flex Real-Time PCR Systems (Applied Biosystems). The relative expression levels of SARS-CoV-2 N gene was calculated using the standard curve method and normalized to 18S ribosomal RNA as an internal control.
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bioRxiv - Microbiology 2021Quote: ... Dissociated cells and remaining pieces of tissue were placed in a single well of a 6-well plate containing 2 mL of Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 20% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Genetics 2021Quote: ... The samples were either frozen (n = 6) or stored in the RNAlater reagent (Thermo Fisher Scientific, Waltham, MA, USA) (n = 4).
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Genetics 2019Quote: Beginning at week 2 post cloning and continuing until week 6 the Phusion Blood Direct PCR Kit (Thermo Scientific) was utilized to identify positive clones ...
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bioRxiv - Genomics 2020Quote: ... standards (3 x 10μl) and samples (6 x ∼18μl) were loaded on a 4-12% Bis-Tris Nu-PAGE (Life Technologies) gel for electrophoresis ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Immunology 2022Quote: ... The presence of SARS-CoV-2 RNA in these samples was evaluated by one-step RT-qPCR in a final volume of 12.5LμL per reaction in 384-wells PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: HeLa cells were grown to 80% confluency in 6-well plates and transfected with 300 ng pcDNA3.1-FLAG-RBM39 constructs using Lipofectamine2000 (Invitrogen). On the following day ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa RAD54KO cells were transfected with 3μg of RAD54-GFP or eGFP plasmid per well in a 6-well plate using Lipofectamine LTX PLUS (Invitrogen). Stable cell lines were generated by selecting with neomycin (800μg/ml ...