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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1.5×105 4T1 cells were co-cultured in 6 wells plates using Transwell plates (0.4 μm, Nunc, ThermoFisher Scientific) with Gr1+CD11b+ sorted cells (top ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.5×105 4T1 cells were co-cultured in 6 wells plates using Transwell plates (0.4 μm, Nunc, ThermoFisher Scientific) with Gr1+CD11b+ sorted cells (top ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were incubated at 37°C for 30 min and loaded onto 6% DNA retardation gels (Thermo Fisher Scientific). DNA was detected by staining the gel with GelRed (VWR) ...
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bioRxiv - Cancer Biology 2022Quote: Total cellular RNA was extracted post-treatment in HT-29 cells (1.5×105/well) plated in 6-well plates (Nunc) at indicated time-points ...
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bioRxiv - Biophysics 2022Quote: ... thoracic and lumbar DRGs were harvested from 4 to 6 week old MrprD-GFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: Modified hESCs were cultured and propagated on Matrigel-coated 6-well culture dishes in Stem Flex medium (Thermo Scientific) supplemented with 100 U/ml Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Genetics 2022Quote: ... Coverslips were mounted with Prolong gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI, P36931, Thermo Fisher Scientific) and analyzed under a Zeiss LSM 510 Meta Confocal microscope (Carl Zeiss ...
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bioRxiv - Microbiology 2022Quote: ... or 6 µL of H2O (negative control) and run in a StepOne™ Real- Time PCR System (ThermoFisher scientific) with the following program ...
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bioRxiv - Molecular Biology 2022Quote: ... 6 replicates of each condition were treated with DMSO or 100 nM dTAG13 (Tocris Bioscience, Fisher Scientific 66-055) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... adult WT C57BL/6J mouse testes were dissected and incubated at 37 °C for 6 h in either Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, Thermo Scientific) or compound 5 (100 μM ...
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bioRxiv - Neuroscience 2022Quote: ... of 0.5% low-melting agarose (FMC) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-diamidine-2-phenylindole dihydrochloride And coverslipped using ProLong™ Gold Antifade Mountant (Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-diamidine-2-phenylindole dihydrochloride And coverslipped using ProLong™ Gold Antifade Mountant (Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Slides with 6 μm sections were mounted in ProLong Glass Antifade Mountant (Thermo Fisher Scientific, Sugar Land, TX, USA) and those with 35 μm sections in SlowFade Glass Soft-set Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were allowed to rest in fresh slicing solution at 4 °C for 30-45 minutes before being mounted on individual semiporous membrane inserts (2 slices per insert) placed in 6 well plates with 1.1mL of culture media [50% Minimal Essential Medium (MEM; Gibco), 25% heat-inactivated horse serum (Gibco) ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Quantitative RT– PCR was performed using the QuantStudio-6 and -7 Flex Real-Time PCR Systems (Thermo Fisher Scientific) with SYBR® Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Plant Biology 2023Quote: ... was employed for reporting successful amplification using a QuantStudio 6 Pro real-time PCR system (Applied Biosystems, Waltham MA) with specific primer pairs for each target NbBBL gene ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed by a QuantStudio 6 Flex real-time PCR detection system (Applied Biosystems, Foster City, CA) with SYBR Premix Ex TaqTM II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2022Quote: Vero cells in 6-well plates were transfected with 1.5 μg/well pQF356 using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Then the cells were seeded in 100 mm plates in medium containing 800 μg/mL G418 to allow colonies to develop for two to three weeks ...
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bioRxiv - Neuroscience 2022Quote: 3D tissue viability was checked at 2 and 6 weeks after seeding following the established Calcein AM (Invitrogen, C1430) staining protocol ...
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bioRxiv - Molecular Biology 2022Quote: Membrane order was assessed using the polarity-sensitive membrane probe Laurdan (6-Dodecanoyl-2-Dimethylaminonaphthalene) (Thermo Fisher Scientific, D250)20 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Bioengineering 2022Quote: ... was buffered to a final concentration of 6 mg/mL at pH 7.4 with 250,000 cells/mL using 10X phosphate buffered saline (PBS) (Thermo Fisher) with phenol red ...
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bioRxiv - Developmental Biology 2022Quote: Lungs from experimental C57BL/6 mice were inflated with an enzyme mix consisting of the following: Leibovitz media (Invitrogen) 2mg/mL of collagenase type 1 and 2mg/mL of elastase (Worthington Biochemical) ...
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bioRxiv - Neuroscience 2024Quote: ... and CHIR 99021 (Cat# 72052) in sterile non-tissue culture treated 6-well plates (Thermo Fisher Scientific, Cat# 150239). NSCs were passaged using either accutase (Stem cell technologies ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: MEF cells were grown in a 6 well plate seeded at 50–60% confluency in 2 ml Dulbecco’s modified Eagle medium (DMEM, Gibco) containing 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... The optical density at 600 nm (OD600nm) was measured with a Genesys 6 spectrophotometer (Thermo Fisher Scientific, United States). The preculture was next diluted into a baffled shake flask (250 ml ...
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bioRxiv - Plant Biology 2024Quote: ... 8 and 6 hours were then transferred to MS medium containing zeocin with Actinomycin D (Thermo Fisher, J60148.LB0) or zeocin with DMSO (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Cells were collected from one well of a 6-well plate using 700 µL of TRIzol Reagent (Life Technologies) and RNA was extracted using Direct-zol RNA Miniprep (Zymo Research) ...
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) plus GlutaMAX (Thermo Fisher Scientific), supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... and 6 µg of psPAX2 plasmids into 293T cells grown in a 10-cm dish using Lipofectamine 2000 (Invitrogen). The cell culture supernatant was collected 48 h after transfection and used to transduce parental HeLa cells in the presence of polybrene (8 µg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were functionalized with 0.1M sulfo-succinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Cat# 22589, Thermo Scientific) dissolved in HEPES before overnight incubation in collagen type-I solution (Catalogue # A1048301 ...
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... The transcribed RNA was then separated on a 6% TBE-Urea (7M urea) polyacrylamide gel (Thermo Fisher Scientific, EC6865BOX) which was post stained with GelRed (Biotium ...
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bioRxiv - Genomics 2024Quote: ... 3 µg of each plasmid was mixed with 6 µl of Plus reagent and 7.5 µl of Lipofectamine LTX (Invitrogen) reagent ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Bone marrow-derived mesenchymal stromal cells from C57BL/6 mice (Cyagen) were cultured in MEMα supplemented with GlutaMAX (Gibco) in 15% fetal bovine serum (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse Transfection of rescue plasmid was performed in 6-well plates using the Lipofectamine 3000 reagent (Thermo Fisher Scientific) to the proportions of 5 μL of 2 μg plasmid per well ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and nuclei were stained with 4’-6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and mounted with an aqueous mounting medium (Aqua-Poly/Mount ...
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bioRxiv - Cell Biology 2024Quote: ... Around 1.6-3.2μg of digested DNA was used to synthesise mRNA with the T3 mMESSAGE mMACHINE kit (AM1348, ThermoFisher Scientific). The mRNA product was purified with the RNeasy MinElute Cleanup Kit (74204 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... live/dead staining was carried out using 6 μM syto 9 and 30 μM propidium iodide (BacLight, Molecular Probes). Biofilms were imaged using a Zeiss LSM 700 compact confocal laser scanning microscope using the 40x objective and appropriate fluorescence settings (syto 9=488 nm laser ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa were prepared in 6-well plates 24 hours before the transfection with 4.5.105 cells/well in RPMI medium (Gibco-Invitrogen) supplemented with 10% fetal calf serum (Gibco-Invitrogen) ...