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Citations for Thermo Fisher :
3051 - 3100 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was grown in Top10 chemical component cells (ThermoFisher), digested with the corresponding enzymes ...
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bioRxiv - Cancer Biology 2021Quote: ... was cloned into plasmid pcDNA5/FRT/TO (Thermo Fisher Scientific) bearing an mCherry and mNeon tandem fluorescent tag (tFT ...
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bioRxiv - Microbiology 2021Quote: ... and was subcloned in the Thymidine kinase Cypridina plasmid (Thermofisher) in-frame with the Cypridina luciferase gene ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids encoding MARK4 was transfected with Lipofectamine 2000 (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... The plasmid was extracted with GeneJET Extraction Kit (Fisher Scientific). The identity of the clones was confirmed by sequencing at Macrogen.
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bioRxiv - Bioengineering 2020Quote: ... The plasmid was extracted with GeneJET Extraction Kit (Fisher Scientific), and sequenced at Macrogen for confirmation of the mutation.
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bioRxiv - Developmental Biology 2022Quote: ... we created recombinant pCRTM4-TopoTM plasmids (Invitrogen, Carlsbad, CA, USA) containing 18S and 28S ribosome RNA PCR amplicons ...
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bioRxiv - Microbiology 2022Quote: ... For subcloning of plasmid pET302/ply006 purified pET302 vector (Invitrogen) and codon optimized synthetic DNA (Gene Art DNA Strings ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Humanized anCas were cloned into pcDNA3.1 plasmid expression vector (ThermoFisher). The sgRNA target sequences were designed with Breaking-Cas web tool62 and cloned into MLM3636 plasmid vector (Addgene #43860 ...
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bioRxiv - Immunology 2022Quote: ... The plasmid was transiently transfected into freeStyle293F cells (Thermo Fisher). Protein was purified from filtered cell culture supernatant by Ni-NTA affinity column first and then by a StrepTactin (IBA ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting plasmids were transiently transfected into HEK293F cells (ThermoFisher). Cells were maintained in FreeStyle 293 medium and transfected using the PEI Max reagent (Polysciences Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... EXOC6-HALO and EXOC7-HALO plasmids (Lipofectamine 2000; 11668019; Invitrogen) according to manufacturers’ instructions ...
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bioRxiv - Biophysics 2019Quote: ... The Wizard SV Gel and PCR Clean-Up System were purchased from Promega while the GeneJet Plasmid Midiprep Kit was purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Plant Biology 2019Quote: ... Plasmids were then extracted following manufacturer’s instructions from Thermo Scientific GeneJET Plasmid Miniprep Kit ...
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bioRxiv - Neuroscience 2019Quote: All plasmids were made using the Gateway system (ThermoFisher, 12538120) with combinations of entry and destination plasmids as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... The plasmids were propagated in DH5α bacterial cells (Life Technologies). The constructs were verified by sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid concentration was measured using Qubit (Thermofisher, catalogue number Q32850). The primers for cloning were ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmids were transfected into cells using Lipofectamine (ThermoFisher, Waltham, Massachussetts). Plasmids psPAX2 and pMD2.G ...
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bioRxiv - Genetics 2020Quote: ... or Myc-Exosc2-G198D (pAC3465) plasmid using Lipofectamine 2000 (Invitrogen) and cells were collected 48 hr after transfection ...
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bioRxiv - Cancer Biology 2019Quote: Cells were transfected with plasmid vectors using Lipofectamine 2000 (Invitrogen). For stable overexpression of FLAG tagged PITX1 ...
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bioRxiv - Cancer Biology 2019Quote: ... or PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA). These were transfected into Tet-On HEK-293TLDcells [10] by incubating 3 µg plasmid DNA with 9 µL Fugene HD (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were performed with 1.5 μl Lipofectamine 2000 (Invitrogen) per 100 μL Opti-MEM media (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: ... We subcloned this region into the pENTR-1A plasmid (ThermoFisher) using KpnI and XhoI restriction sites on either side of the promoter ...
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bioRxiv - Microbiology 2020Quote: ... Maxipreped plasmids were transiently transfected into 293-F cells (Thermofisher) and the cells were further cultured in suspension for 6 days before harvesting antibody-containing supernatant ...
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bioRxiv - Systems Biology 2020Quote: ... The GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, USA) was used for plasmid isolation ...
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bioRxiv - Microbiology 2020Quote: ... Spike expression plasmids were transfected with Lipofectamine LTX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid DNA concentration was measured by fluorometric quantification (Qubit, ThermoFisher) and copy number was normalized between samples in the same experimental condition according to the M.W ...
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bioRxiv - Microbiology 2020Quote: The plasmid pMR-RQ-Bb/SA11g72AFLUnaG was obtained from ThermoFisher and contained a modified synthetic cDNA of SA11 segment 7 (NSP3) ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg plasmid DNA was added with Lipofectamine 3000 (Invitrogen), 50 μl/ml OPTI-MEM (Gibco)/DMEM ...
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bioRxiv - Genomics 2021Quote: ... Plasmids and 60 µl Lipofectamine 2000 reagent (Thermo Fisher Scientific) were each diluted in 1 ml of OptiMEM ...
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bioRxiv - Immunology 2021Quote: Plasmids were transfected into Expi293F cells (ThermoFisher Scientific, Waltham, MA) in a 1:1 ratio of heavy chain:light chain using Expifectamine according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and isolated using a GeneJET plasmid miniprep kit (ThermoFisher, K0503). Sequencing was performed by MCLAB (Molecular Cloning Laboratories).
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bioRxiv - Cell Biology 2019Quote: ... The control GFP-NLS plasmid was from Invitrogen (V821-20). To knock down expression of RTN4 ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmid mistures were diluted in 3 ml OptiMEM (Life Technologies) and supplemented with 60 μl Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Parallel transfection with an eGFP expression plasmid (240 ng; Invitrogen) was used to determine nucleofection frequencies (>90%) ...
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bioRxiv - Genetics 2020Quote: The TyrgRNA-Tomato plasmid was purchased from GenScript and prepared using PureLink™ HiPure Plasmid Midiprep Kit (Invitrogen). Plasmid (10 ng/µL) ...
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bioRxiv - Cell Biology 2021Quote: ... miniprepped DNAs using PureLink™ Quick Plasmid Miniprep Kit (Invitrogen) were analyzed by Sanger sequencing.
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection was performed using Lipofectamine reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2021Quote: ... the plasmid was transfected using Lipofectamine 2000 (Thermo Fisher Scientific) into BHK-21 cells grown in GMEM with 2% FBS and 10% TPB at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... PCR product was mixed with the donor plasmid pDONR207 (Invitrogen), and subjected to a recombination reaction with Invitrogen Gateway BP Clonase™ ...
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bioRxiv - Cell Biology 2021Quote: ... PShuttle-CMV plasmids were then digested overnight with MssI (ThermoFisher) and linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... coli using a PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher) and used to transfect Sf9 cells using Cellfectin II transfection reagent (Thermo Fisher) ...
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bioRxiv - Biochemistry 2021Quote: ... and GeneJET Plasmid Miniprep Kit were bought from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was transformed into OneShot Top10 competent cells (Invitrogen). Oligonucleotides were synthesized by Integrated DNA Technologies.
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bioRxiv - Synthetic Biology 2021Quote: ... The pT7-CFE-GFP plasmid was purchased from Thermo Fisher Scientific and prepared at different concentrations.
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bioRxiv - Neuroscience 2021Quote: Plasmid and Si-RNAs were transfected with Lipofectamine 2000 (Invitrogen) and RNAi max (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... subcloned into the pCR™2.1-TOPO® plasmid (Thermofisher), and 5–7 clones were sequenced in both directions by Sanger sequencing at Retrogen® and/or Genewiz® to identify both alleles in a each fly ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids were isolated using a mini prep kit (Thermo Fisher). DNA concentration was measured on a NanoDrop (Thermo Fisher ...
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bioRxiv - Biophysics 2020Quote: ... Cells were transfected with plasmids using Lipofectamine 3000 (Life Technologies) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2021Quote: ... and 50 ng of attD plasmid using Lipofectamine 2000 (Invitrogen). 2-3 days after transfection of cells with all three plasmids ...