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Citations for Thermo Fisher :
2851 - 2900 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... HEK293T/17 cells at sub-confluence in 6-well plates or 100 mm dishes were transfected with HLA-DRA plasmid or HLA-DRB1 plasmid or a 1:1 combination of both using the Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, UK) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Cell Biology 2021Quote: The human MISP sequence harbored in a pCMV-SPORT plasmid (Harvard PlasmID Database; HsCD00326629) was subcloned by PCR and TOPOcloned into a pCR™8 Gateway entry vector (Invitrogen; 46-0899). In-frame sequence insertion was confirmed by sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... LINC000881 overexpression was achieved by lipofectamine based transfection of LINC00881 plasmid versus bacterial alkaline phosphatase control plasmid using RNAiMAX reagent (ThermoFisher Scientific, Waltham, MA). iPSCs were harvested at 48-72 hours following transfection for functional and gene expression studies ...
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bioRxiv - Microbiology 2021Quote: ... jejuni PglB was amplified by PCR from the plasmid pACYCpgl with the pTac promoter and LacIq repressor from plasmid pEXT20 as a template using Pfx Polymerase (Thermo Fisher Scientific UK) with (SEQ ID 15 ...
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bioRxiv - Immunology 2022Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (ThermoFisher Cat No. 12347019). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Molecular Biology 2019Quote: To generate linear plasmids without telomeric ends, the circular plasmids were amplified with primers (BP1204, BP1205; Table 2) using Phusion polymerase (Thermo Fisher Scientific, USA). Cycling conditions were ...
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bioRxiv - Synthetic Biology 2022Quote: ... we developed a vacuum-based plasmid extraction protocol named “TeVacS” on Fluent using the PureLink™ Pro Quick96 plasmid purification Kit (K211004A) (Invitrogen, Carlsbad, CA). Our customized protocol features the use of vacuum manifold for efficient removal of washing buffer and final elution of plasmid DNA into a 96-well plate placed on a 3D-printed holder ...
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bioRxiv - Developmental Biology 2022Quote: ... 90 ng of ASD library plasmids and 30 ng of control library plasmids were used for electroporation of MegaX DH10B T1R Electrocomp™ Cells (Thermofisher, cat.no. C640003) following the manufacturer’s guide ...
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bioRxiv - Physiology 2022Quote: ... HPAECs were transfected with either control plasmids (empty vector) or 1μg of HA-ubiquitin plasmids (HA-tagged Ubi) using Lipofectamine 3000 (Thermo fisher Scientific, PA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... a TMPRSS2 plasmid and S plasmids from SARS-CoV-2 variants into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) 39,40 ...
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bioRxiv - Physiology 2022Quote: Human TRPM6 was either transfected in a bicistronic vector pCINeo/IRES-GFP plasmid or in a pcDNA pDEST53 plasmid (ThermoFisher Scientific, Waltham, MA) (26 ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Genetics 2024Quote: ... A subset of the saturated culture was used to extract purified plasmid DNA using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, K0503).
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bioRxiv - Biophysics 2022Quote: ... 2 μg total plasmid DNA (725 ng CagFbFP-Q148V plasmid + 1275 mg MTS-CagFbFP plasmid) and 2 μl PLUS Reagent were diluted in 100 μl Opti-MEM (Thermo Fisher Scientific, USA), and 4 μl Lipofectamine LTX were diluted in 100 μl Opti-MEM ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected by transfer plasmid and packaging plasmids psPAX2 (addgene #12260) and pMD2.G (addgene #12259) using Lipofectamine 3000 reagent (Thermo Fisher Scientific # L3000015). Viral particles were concentrated using Lenti-X Concentrator (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
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bioRxiv - Molecular Biology 2024Quote: ... HEK 293T cells were transiently co-transfected with individual expression plasmids encoding full-length RAI2 and CtBP1 and the reporter plasmid using Lipofectamine 2000 (ThermoFisher Scientific, MA, USA). Co-transfection with the pEGFP-N2 plasmid (Takara Bio ...
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bioRxiv - Neuroscience 2024Quote: ... prior to the expansion of resistant colonies in 100 mL of Luria-Bertani (LB) broth and purification of plasmid DNA using a PureLink HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific; #K210014), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... or pMK-RQ (EtIMP1Cit) plasmid with appropriate restriction enzyme sites for cloning into pYD1 yeast display plasmid vector (Invitrogen, Thermofisher Scientific, Waltham, MA, USA). A NotI restriction site was included between each antigen sequence and the 3’citrine tag ...
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bioRxiv - Immunology 2020Quote: ... 1 × 105 cells were transfected with the appropriate plasmid combinations or plasmid/siRNA combinations using the Neon Transfection System (Thermo Fisher, Waltham, MA, USA) and a 10 µL Neon tip at 1000 V ...
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bioRxiv - Immunology 2022Quote: ... 12μg heavy chain plasmid and 12 μg of light chain plasmid were added into 3ml of Opti-MEM™ (Thermo Fisher Scientific cat.# 31985070), after inverting ...
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bioRxiv - Cancer Biology 2024Quote: ... Retrovirus was generated by transfecting oncogenic plasmids and viral packaging plasmid (pCL-Ampho) into phoenix cells with Lipofectamine 2000 (Life Technologies, Grand Island, NY). Viral particles were incubated with prostate cells (70% confluence ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 100 ng/well of either TOP luciferase reporter plasmid or the negative control FOP plasmid using Lipofectamine™ LTX Reagent with PLUS™ Reagent (Invitrogen, Carlsbad, US) in serum-free Opti-MEM® medium (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... Sequence-verified Cas9-gRNA-Puro plasmids and pMA donor plasmid were co-transfected into in-house C57BL6/N 6.0 ESC using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) prior to selection with 2 μg/ml puromycin for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... The generated transfer plasmid was sequence confirmed and a maxiprep was prepared using PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification kit (Thermo Fisher Scientific, A31231). For AAV6 production ...
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bioRxiv - Microbiology 2020Quote: ... slides were incubated with secondary goat anti-rat IgG (H+L) Alexa Fluor 488 (1:500; Invitrogen) and goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... BM endothelial cells were immunopurified from cell suspensions using sheep anti-rat IgG Dynabeads (ThermoFisher Scientific 11035) pre-captured with a CD31 antibody (MEC13.3 ...
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bioRxiv - Cancer Biology 2022Quote: ... as primary antibody and Alexa Fluor® 647 goat anti-rat (dilution 1/200, Invitrogen, cat. A21247) as the secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... the slides were incubated with the following secondary antibodies (anti-rat IgG Alexa Fluor 594, Molecular Probes A-11007 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in goat anti-rat Alexa 568 secondary antibody (1:500, #A11077, ThermoFisher, RRID: AB_2534121) and donkey anti-rabbit horseradish peroxidase conjugated secondary antibody (1:500 ...
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bioRxiv - Genomics 2019Quote: ... as a secondary antibody for the GAPDH antibody and Goat anti-Rat IgG (H+L) (Invitrogen 31470) as a sec-ondary antibody for the CTD antibody ...
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bioRxiv - Immunology 2019Quote: ... followed by goat-anti-rat secondary antibody-Alexa Fluor® 800 conjugate (0.33 ng/ml; ThermoFisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... rat monoclonal α-Elav 1:10 (Developmental Studies Hybridoma Bank) and rabbit α-GFP 1:500 (Invitrogen).
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies used were anti-mouse or anti-rat Alexa 568 at 1:400 (Thermo Fisher Scientific). Nuclei were labeled using 0.05 μg/ml DAPI (Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: ... and anti-E-cadherin (rat, 1:50; formaldehyde-hand peeled fixation; DHSB) antibodies and AlexaFluor568 phalloidin (Invitrogen) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... This was followed by incubation with Alexa555-conjugated anti-rat and Alexa488-conjugated anti-mouse (Molecular Probes). DNA fibers were studied in a LSM780 confocal microscope using a 64x oil objective and lengths of CldU and IdU tracks were measured using the ImageJ software (http://fiji.sc/Fiji) ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were: Alexa Fluor 488 anti-mouse and Alexa Fluor 555 anti-rat (Fisher Scientific, ref.A11029 and ref ...
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bioRxiv - Cell Biology 2021Quote: ... 105 cells/ml were re-suspended in 500 µl of 2.3 mgs/ml collagen rat tail (Gibco). A 100 µl volume of the collagen and cell mixture was dispensed in quadruplicate wells onto poly-D-Lysine (0.1mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... rat and rabbit IgG or chicken IgY are obtained from Invitrogen (A-32723, A-11034, 1:300) or Jackson ImmunoResearch Laboratory (711-165-152 ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa-Fluor goat anti-rabbit 680 and Alexa-Fluor goat anti-rat 800 (all from Life Technologies). The membrane was imaged using the LiCor Imaging System and Odyssey V3.0 software (LiCor) ...
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bioRxiv - Neuroscience 2020Quote: ... Followed-by Goat anti-Rat IgG (H+L) Secondary Antibody HRP (Thermo Scientific, USA, # 31470 1:5,000), Goat anti-Rabbit IgG H&L (IRDye® 800CW ...
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bioRxiv - Cell Biology 2021Quote: ... alone or together with Alexa Fluor 647-conjugated goat anti-rat secondary antibody (A-21247, Invitrogen, USA) or Alexa Fluor 647-conjugated goat anti-rabbit secondary antibody (A-21245 ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Physiology 2022Quote: ... -rat and –guinea pig secondary antibodies at RT for 1 hour on a nutator (1:500, ThermoFisher, #A21206 ...
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bioRxiv - Neuroscience 2022Quote: ... and for 48 hours for staining with rat α NG2 (1:250; Thermo Fisher Scientific: MA5-24247). Other primary antibodies used include guinea pig α Vglut2 at 1:1000 (Sigma ...
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bioRxiv - Physiology 2022Quote: ... Diluted primary antibody (100 µL of 1:200 rat anti-rabbit a-tubulin, Clone YL1 / 2, Invitrogen) was pipetted onto the Parafilm and the coverslips were placed cell-side down on top of the solution and incubated at 4 °C for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Flat mounts were washed and incubated in Alexa Fluor 488 (1:500, goat anti-rat, Invitrogen, A110006) for up to 2 hours on a shaker at room temperature ...