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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of this mix was added into wells of a 12-well 6% DNA retardation gel (Invitrogen) that was pre-running for at least 30 minutes in 0.5X TBE at 150V ...
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bioRxiv - Developmental Biology 2024Quote: ... for quantitative reverse transcription PCR analysis on the StepOne/QuantStudio 6 Flex Real Time PCR Systems (Applied Biosystems). The housekeeping gene GAPDH was used as an endogenous control ...
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bioRxiv - Cell Biology 2024Quote: We seeded HEK293T cells at 2.5 × 10^6 cells per 15 cm plate in DMEM (Thermo Fisher 11965118), enriched with 10% FBS (Atlanta Biologicals S11150H) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen, Cat. No. EC6265BOX) to retain only the small RNA inserts ...
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bioRxiv - Cell Biology 2024Quote: ... Z-stack images were taking on day 6-7 after seeding using EVOS™ 7000 Imaging System (Invitrogen). and used for quantification of organoids.
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bioRxiv - Biochemistry 2024Quote: ... 6 second blot time and 10 second wait time before plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) set at 100% humidity and 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... RC Dialysis tubing (3.5 kDa or 8 kDa molecular weight cut-off, Spectrum Spectra/Por 6, Fisher Scientific), HCl (2M ...
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bioRxiv - Cancer Biology 2024Quote: ... and qPCR was performed using the QuantStudio 6 Flex Real-Time PCR System with SYBR green (ThermoFisher Scientific). Expression was normalized to TBP ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
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bioRxiv - Plant Biology 2023Quote: ... and qPCR was performed using the indicated oligos (Table S4) (QuantStudio 6 Pro PCR system; Thermo Fisher Scientific) using Power SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... droplets were put in 6-well Ultra Low Attachment Plates (Thermo Fisher Scientific P6 : REF 150239 “untreated Nunc”) in Expansion Medium without vitamin A (for 50 mL ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed by running the following thermocycler program on a QuantStudio 6 Flex system (Applied Biosystems): 50C for 15 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Gut orientation was analyzed by treating MZ myo1dpt31cand wildtype larvae with 0.3 μg/ml PED-6 (ThermoFisher #D23739) for 3 hours at 6 days post fertilization as described88 ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, USA).
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bioRxiv - Pathology 2023Quote: ... Culture identity was confirmed using species-specific qPCR assays on the Quantstudio 6 platform (Applied Biosystems, MA USA); see Table S1.
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bioRxiv - Molecular Biology 2022Quote: ... HeLa were prepared in 6-well plates 24 hours before the transfection with 4.5.105 cells/well in RPMI medium (Gibco-Invitrogen) supplemented with 10% fetal calf serum (Gibco-Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected as follows: 2 μg eGFP-SHLD3 constructs and 6 μl Lipofectamine 2000 (Thermo Scientific) were incubated in Opti-MEM for 20 min at room temperature before being added to the cells whose media had been changed to McCoy’s with 10% FBS and no antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... 800,000 cells were seeded into a 6-well plate (NuncMicroWell Plates with Nunclon; ThermoFisher Scientific, Waltham, MA, USA). At day 0 the transfection was done ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting pellet was resuspended and differentiated for 6 days in culture media containing: DMEMF12 (Gibco, #11330-032) supplemented with 20% FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... when floating cells were collected and plated into new 6 cm dishes with Maturation Media (RPMI (Gibco, 61870036), 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... thetaiotaomicron using a Applied Biosystems QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, Foster City, CA, USA), primer sets BT2156-BT2160 ...
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bioRxiv - Developmental Biology 2023Quote: ... were isolated from embryos incubated for between 6 (E6.0) and 8 (E8.0) days and maintained in chilled Leibovitz’s L-15 media (GIBCO, Invitrogen). Papillae were dissected as described previously 65 and cultured nerve-side-down on Millicell cell culture inserts (Millipore ®) ...
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bioRxiv - Bioengineering 2023Quote: ... Droplets of a 5 μL cell-Matrigel mixture were manually deposited onto a high-performance #1.5 glass-bottom 6-well plate (0.170±0.005 mm) (Fisher Scientific). The datasets were separated into non-treatment and treatment groups ...
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bioRxiv - Neuroscience 2023Quote: ... 6-well plate (500x103 cells/well) in Neural Expansion Medium (NEM: 49% Neurobasal, 49% Advanced DMEM (Thermo Scientific), 2% Neural Induction Supplement ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were seeded on one well of a 6-well plate coated with poly-Ornithine/Laminin (Thermo Scientific). Medium was changed every other day ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in Cellvis 6 Well glass bottom plates with high performance #1.5 cover glass (Fisher Scientific). For control wells to be pre-treated with cathepsin inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no ...
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bioRxiv - Neuroscience 2023Quote: ... transfections were performed directly after splitting the cells in 6-well plates using Lipofectamine 2000 Reagent (Invitrogen, 11668019) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Grids were blotted for 4-6 seconds with blotforce of 15 on a Vitrobot Mark IV Vitrobot (ThermoFisher) and directly plunged into liquid-nitrogen-cooled liquid ethane.
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bioRxiv - Cancer Biology 2023Quote: ... This insert creates a clean cell-free gap in a 6-or 12-well plate (Thermo Scientific, 150239). The assay was performed according to the manufacturer’s protocol (ibidi) ...
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bioRxiv - Cancer Biology 2023Quote: ... B6.Cas9 keratinocytes(see above) were plated in 6-well plate (Thermo Scientific Nunclon TM Delta Surface; 140675) at 1.5 × 105 cells per well and infected with 100-300μl of low-titer virus in the presence of infection mix (1/10 dilution of polybrene [10 mg ml−1 Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... approximately 10 female and 6 male flies were placed into a flask (Fisher Scientific, Cat. No.:AS-355) of food and maintained at 25°C for one week to allow for mating and egg laying ...
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bioRxiv - Molecular Biology 2023Quote: ... U2OS cells were infected (24 h) in viral mixture (1.5 mL DMEM, 1.5 mL viral harvest, 6 µL polybrene, Invitrogen) and DMEM with 7.5 µg/mL blasticidin (Sigma ...
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bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...
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bioRxiv - Genomics 2023Quote: ... and 6 mg magnetic beads (per sample) were conjugated overnight using the Dynabeads Antibody Coupling Kit (Invitrogen, 14311D). The next day ...
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bioRxiv - Neuroscience 2023Quote: Eight-micron sections of L4-6 DRG were blocked for two hours in Superblock (ThermoFisher; Grand Island, NY), 1.5% Normal Donkey Serum ...
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
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bioRxiv - Immunology 2022Quote: ... The resulting monocytes were then differentiated with 50 ng/ml human M-CSF (Miltenyi) for 6 days in Iscove’s modified Dulbecco’s medium (Gibco) containing 5 % fetal calf serum (CAPRICORN ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitated DNA was analyzed by qPCR on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with Power SYBR Green PCR Master Mix and calculated as % of input ...
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bioRxiv - Cancer Biology 2022Quote: ... Target genes were amplified using PowerUp SYBR Green Master Mix and a QuantStudio 6 thermocycler (Thermo Fisher Scientific). Gene expression was calculated using the ΔΔCt method for candidate genes ...
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bioRxiv - Cell Biology 2023Quote: ... Slide Book Software 6.0 (Intelligent Imaging Innovations) and QuantStudio 6 Real-Time PCR Software (Applied Biosystem, Thermo Fisher). *P<0.05 ...
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bioRxiv - Genomics 2023Quote: ... was added and 10μL of mixture was loaded into 6% DNA Retardation Gels (cat. EC6365BOX, Thermo Fisher Scientific). Gels were run at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The real-time qPCR reactions were performed using the QuantStudio 6 Flex real-time PCR system (Applied Biosystems), of which the parameters were set to 50°C for 2 min ...
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bioRxiv - Zoology 2023Quote: ... The samples were stained by the 4’,6-diamidino-2-phenylindole (DAPI) and the rhodamine-conjugated phalloidin (ThermoFisher). The signals were detected by fluorescent microscopy (Nicon ...
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bioRxiv - Immunology 2023Quote: ... PCSK9 peptides were conjugated to Qß bacteriophage VLPs using succinimidyl 6-((ß-maleimidopropionamido)hexanoate) (SMPH; Thermo Fisher Scientific), as described previously60 ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were washed 4 times with PBST and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: Transient transfections in HeLa and Rme-6-ve cells were performed using either Polyfect or Lipofectamine3000 (ThermoFisher, Ltd), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
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bioRxiv - Cell Biology 2023Quote: Cells cultured in 6 well plates were washed with PBS and fixed with 4% Paraformaldehyde (11400580, Fisher Scientific) in PBS for 5min at room temperature (RT) ...