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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Cells were maintained on 6-well plates or cell culture flasks coated with Attachment Factor Protein (Gibco cat. No. S006100) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... The cells were thoroughly washed again followed by fixation and permeabilization (eBioscience™ Foxp3/Transcription Factor Staining Buffer Set, Invitrogen) for 30 min on ice in the dark ...
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bioRxiv - Genomics 2023Quote: ... Cells were then stained for cell surface markers followed by intracellular staining using the Transcription Factor Staining Buffer Set (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were then fixed and permeabilized using either the Foxp3 /transcription factor staining buffer set (#00-5523-00, ThermoFisher Scientific) or the BD fixation/permeabilization solution kit (#554714 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Splenocytes were incubated at 4°C and washed three times with FACS buffer and fixed with FoxP3/Transcription Factor Staining Buffer Set (Invitrogen) fixative and stored at 4°C until intracellular staining ...
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bioRxiv - Immunology 2023Quote: ... Surface-stained cells were fixed and permeabilized using the eBioscience FoxP3 / Transcription Factor Staining Buffer Set (Invitrogen, #00-5523-00), stained for intracellular cytokines of interest and analyzed on a Cytek Aurora flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed and permeabilized with either the FoxP3/Transcription Factor Staining Buffer Set (eBioscience/Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: ... Whole conditioned media from flasks and bioreactors was 0.2 μm filtered prior to first round UC at an RCFmax of 133,900 x g (Sorvall WX ultraCentrifuge, ThermoFisher; Fiberlite F37L-8×100 Fixed-Angle Rotor, ThermoFisher, k factor=168; PC Bottle Assembly 70 mL, ThermoFisher) for 1 hour at 4° C ...
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bioRxiv - Immunology 2023Quote: We used the cytokine/chemokine/growth factor 45-Plex Human ProcartaPlex™ Panel 1 (Cat# EPX450-12171-901, ThermoFisher, USA) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed and permeabilized with either the FoxP3/Transcription Factor Staining Buffer Set (eBioscience/Thermo Fisher Scientific, Waltham, MA) or BD Cytofix/ Cytoperm Kit (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and lamina propria suspensions fixed for 10 minutes using the Foxp3/Transcription factor staining set (Thermo Fisher 00-5523-00). Cells were then resuspended in 1x permeabilization buffer (Thermo Fisher 00-8333-56 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated with staining buffer containing anti-human BV711-CD11b at 1:20 for 30 min on ice before fixation with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (00-5523-00, ThermoFisher), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Immunology 2024Quote: ... We washed the cells twice before fixing and permeabilizing cell membranes using the eBioscience FoxP3/Transcription Factor Staining Buffer Set (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... MTA obtained) iPSCs expressing six inducible transcription factors (MAFB, CEBPα, IRF, PU1, CEBPβ, IRF5) were dissociated using StemPro Accutase (Gibco) and plated in Induction Medium (Essential 8 Medium,10 μM Chroman1 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with the full-length HMGA2 gene cloned under the control of the elongation factor promoter into the expression vector pEF6 (Invitrogen). Stable transfectants were selected in medium supplemented with 10 μg/ml blasticidin ...
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bioRxiv - Immunology 2024Quote: ... chronic infected patient and HD groups by Luminex using the Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (#EPX450-12171-901 ThermoFisher) according supplier’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... iPSC clusters were extracted followed by culturing in neural induction medium with growth factors including B27 Supplement (Gibco: 17504-044), N2 supplement (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... The iPSCs were resuspended in human embryonic stem media with 10 ng/mL of bFGF (beta fibroblast growth factor, Invitrogen) (HES-10 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-©tubulin (mouse monoclonal, MilliporeSigma T6557, 1:200) and mouse anti-ZO-1 (Life Technologies, 1:300). Rabbit anti-Pard3 (rabbit polyclonal ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 to 2 µg of RNA was reverse transcribed with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using random hexamers for amplification of cellular genes or gene specific primers for amplification of either minus or plus strand viral RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 2 μg of total RNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The expression of 370 ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA (2 µg) was then reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems; ThermoFisher Scientific). RT-qPCR was then performed via the CFX384 Real Time system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA (2 µg) was then reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems; ThermoFisher Scientific). RT-qPCR was then performed via the CFX384 Real Time system (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µg of the extracted RNA were reverse-transcribed using the high capacity cDNA reverse transcription kit (Applied Biosystems) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 0.5 to 2 µg of RNA was reverse transcribed with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using random hexamers for amplification of cellular genes or gene specific primers for amplification of either minus or plus strand viral RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit containing SuperScriptIII® (2 units/µL; Invitrogen, Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit containing SuperScriptIII® (2 units/µL; Invitrogen, Life Technologies).
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bioRxiv - Bioengineering 2021Quote: ... DNA quality was assessed using a 2% agarose gel and purified using the GeneJet PCR kit (Thermo Fisher Scientific). DNA was then eluted to a concentration of between 25-100 ng/µL and amplified using the T7 MegaShortScript RNA synthesis kit (Ambion ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 2 μg RNA per sample using RevertAid First Strand cDNA Synthesis kit (Thermo Fisher). PR1 expression was detected using primers qPR1_F and qPR1_R by quantitative real-time PCR using Maxima SYBR green mix (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of EdU (5-ethynyl-2’-deoxyuridine) was performed as per the manufacturer’s guidelines using Click-it EdU Alexa Fluor 488 imaging kit (Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of RNA were reverse transcribed using the Revert-Aid™ H Minus Reverse Transcriptase Kit (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2019Quote: ... The complementary DNA was generated from 2 µg total RNA by RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific). Quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µL of tricolor calibration beads (Invitrogen™ MultiSpeck™ Multispectral Fluorescence Microscopy Standards Kit, Life Technologies Europe B.V.) was added to the sample ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed as per to manufacturer’s instructions with 4μl of lysis buffer (Cisbio phosphor-ERK1/2 cellular assay kit, Invitrogen, UK) for 30mins shaking at room temperature ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µL were used to measure protein concentration by a BCA protein assay kit (Thermo Fisher Scientific, Roskilde, Denmark).
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bioRxiv - Cell Biology 2020Quote: ... cDNA was prepared from 2 gg of total RNA using SuperScript™ III reverse transcriptase kit (ThermoFisher, 18080-044) with Oligo(dT)20 Primer (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... The extracted RNA (1-2 μg) was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR was carried out in the BioRad CFX96 Real-Time System using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... baltica strain #1432 was extracted from 2 mL of overnight culture by Genomic DNA Purification Kit (Thermo Fisher Scientific) according to manufacturer’s protocol for Gram-negative bacteria ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg RNA was reverse transcribed into cDNA using a RevertAid first-strand cDNA synthesis kit (ThermoFisher, USA) supplemented with Oligo(dT)18 and Random Hexamer Primer ...
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bioRxiv - Immunology 2021Quote: ... Products were quantified by analyzing 2 μl with the Quant-iT dsDNA High-Sensitivity Assay Kit (Thermo Fisher, Q331120). Twenty ng of purified PCR product was used as input for library construction using the NEBNext Ultra II FS DNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of total RNA was determined (Supplementary Table 2) using the Qubit® RNA Assay Kit (Life Technologies). For rRNA depletion ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 detection was performed by quantitative real time PCR using Taqpath COVID-19 combo kit (Applied Biosystems, ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: Beginning at week 2 post cloning and continuing until week 6 the Phusion Blood Direct PCR Kit (Thermo Scientific) was utilized to identify positive clones ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, ThermoFisher Scientific) according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, ThermoFisher Scientific) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) was incubated using Click-iT™ Plus EdU Cell Proliferation Kit (Thermo Fisher Scientific) after DAPI staining ...