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Citations for Thermo Fisher :
251 - 300 of 4283 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were cultured in DMEM (Life Technologies), supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC) were grown in DMEM (Gibco) with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Cell Biology 2021Quote: ... The agarase-treated samples were then carefully poured into combing reservoirs containing 1.2 ml of MES solution supplemented with 2 mM Zn(O2CCH3)2 and either S1 nuclease (40 U/ml) or S1 nuclease dilution buffer (Thermo Fisher) and were incubated for 30 min at RT ...
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bioRxiv - Genomics 2023Quote: ... the plate containing the lysed cells is supplemented with RNA spike-ins (1 μL of a 1:1,600,000 dilution of ERCC spike-in mixture A (Life Technologies)) ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Immunology 2022Quote: ... 2% FCS (ThermoFisher), and 50 μg/mL gentamicin (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 4 % FCS (Gibco), 200 units/mL penicillin and 0.2 mg/ mL streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS (InVitrogen) and either 1 µg/mL puromycin (DIvA cells ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Gibco), 1% non-essential amino acid (NEAA ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco) in RPMI ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% FCS (Invitrogen), 5% human serum (HS) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (Gibco), 1X Pen-Strep (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS (Gibco), 25 mM HEPES (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... 10 % FCS (Gibco), 1 % PenStrep (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... 44% FCS (Gibco), and 6% DMSO (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... 5% FCS (Gibco), and 1x penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... 10% FCS (Gibco), penicillin/streptomycin ...
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bioRxiv - Genetics 2020Quote: ... or HA-Tag (Invitrogen) antibody for 1 h at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... TMT10plex tags (Thermo Scientific) were dissolved in anhydrous ACN and added to the peptide sample in a 1:10 peptide:TMT ratio ...
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bioRxiv - Biochemistry 2023Quote: ... anti-SNAP tag (Invitrogen), and DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Biochemistry 2022Quote: ... followed by C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For SARS-CoV-2 RBD VOCs proteins ...
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bioRxiv - Immunology 2022Quote: ... followed by a foldon trimerization motif and C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For competition ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... The gel was then stained using Pro-Q Emerald 300 staining for glycoproteins kit (Invitrogen) following the procedure described by the supplier.
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Viral copy number quantification by RT-qPCR was performed using Detection Kit for 2019 Novel Coronavirus (2019-nCoV) RNA (PCR-Fluorescent Probing) (Cat. #DA-930) (China) in a QuantStudio 3 real-time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Microbiology 2021Quote: ... B.1.617.2 Spikes were generated by overlapping PCR using a codon-optimized wild-type SARS-CoV-2 Spike gene (GeneArt, ThermoFisher) that was synthesized (Biobasic ...
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bioRxiv - Molecular Biology 2020Quote: ... To 1/3 of the sample 1/10 of the recommended amount of spike-in (ERCC RNA spike-in mix, Ambion) was added ...
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bioRxiv - Immunology 2021Quote: ... Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+Spike+Spike+ cells were sorted into individual wells containing RNase OUT (Invitrogen), First Strand SuperScript III buffer ...
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bioRxiv - Immunology 2023Quote: RNA was isolated using TRIfast [46] (Peqlab) with pre-isolation D.melanogaster Schneider S2 spike-in (1:10) and post-isolation ERCC spike-ins (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
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bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
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bioRxiv - Genomics 2020Quote: ... and A3B3 (Table S1) were synthesized (Thermo Fisher Scientific) and cloned into BamHI/XbaI restriction sites of the pcDNA3.1(+ ...
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bioRxiv - Synthetic Biology 2019Quote: ... S1 nuclease was added (0.2 units; Thermo Fisher Scientific) and samples were incubated at 37 °C for 10 minutes and quenched in a solution of 7 M Urea and 10 mM EDTA ...
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bioRxiv - Pathology 2019Quote: ... All primer sequences (Table S1) were synthesized by Invitrogen Biological Company (Shanghai ...
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bioRxiv - Cell Biology 2021Quote: S1/S2 basal media: 500 mL MCDB131 (Life Technologies) supplemented with 0.75 g NaHCO3 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... then digested with T7 Exonuclease and S1 Nuclease (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-S (S1) (PA5-81795, Thermo Fisher); TMPRSS2 (sc-515727 ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with the S1 nuclease (18001-016, ThermoFisher Scientific) at 20 U/mL in S1 buffer (30 mM sodium acetate pH 4.6 ...
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bioRxiv - Genetics 2021Quote: ... ERCC ExFold RNA Spike-In mixes (Thermo Scientific) were added as internal control ...
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bioRxiv - Genomics 2020Quote: ... ERCC RNA Spike-In (ThermoFisher Cat. No. 4456740) was diluted in the lysis mix following the manufacturer’s user guide and previous studies36 ...
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bioRxiv - Microbiology 2020Quote: ... Spike RBD or NP in 1X PBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... ERCC RNA Spike-In mixes (Thermo Fisher Scientific)11 ...
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bioRxiv - Pathology 2020Quote: ... and SARS Spike protein (Thermo Fisher, 1:250), and with Phalloïdin-647 (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: Prior to library preparation ERCC spike-ins (Invitrogen) were added proportionally between the cytosolic and ER fraction in each condition ...
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bioRxiv - Cell Biology 2020Quote: ... including ERCC RNA spike-ins (ThermoFisher Scientific# 4456740). Bulk controls were also processed for each C1 run using 100 cells and the same reagent mixes as used for the C1.