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Citations for Thermo Fisher :
451 - 500 of 4283 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: HEK293 cells were grown and maintained in DMEM (Thermo Fisher), supplemented with 10% FBS (PAN-Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and Hela cells were cultured in DMEM media (Invitrogen) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293 and P19 cells were transfected by Lipo2000 (Invitrogen, 11668500) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... suspension HEK293 host cell line (Freestyle 293-F, ThermoFisher Scientific) was grown in Freestyle 293 expression medium (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 Flp-In™ T-REx™ cells (Thermo Fisher) were co-transfected with the final plasmid and the pOG44 plasmid (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... was transduced into HEK293 cells using Lipofectamine LTX (Invitrogen-ThermoFisher) to knock out PML by the CRISPR-Cas9 system ...
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bioRxiv - Cell Biology 2023Quote: ... was transduced into HEK293 cells using Lipofectamine LTX (Invitrogen-ThermoFisher) to knock out PML by the CRISPR-Cas9 system ...
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bioRxiv - Neuroscience 2023Quote: HEK293 and GL261 cells were cultured in DMEM (Gibco, #31885) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... GKO HEK293 cells were maintained in DMEM (Gibco Cat# 11995065) containing 10% FBS (Gibco Cat# 16000044) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 Flp-In T-Rex cells (Thermo Fisher Scientific, R78007) were transfected with pcDNA5/FRT/TO-GFP (see below ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 Flp-In T-REx cells (Thermo Fisher Scientific, R78007) with SBP-tagged eIF4A1 integrant 15 were seeded in a 10-cm dish and cultured for 3 d in the presence of 1 μg/ml tetracycline ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 in Dulbecco’s modified Eagle Medium (DMEM, Life Technologies #11965118); T-47D in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Cell Biology 2023Quote: Flp-In™ T-REx™ HEK293 cells (Invitrogen, R78007) containing a single genomic FRT site and stably expressing the Tet repressor were cultured in DMEM medium supplemented with 10% FBS ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cell lines were lifted using TrypLE Express (Thermo Fisher) and resuspended in media supplemented with the appropriate volume of cADDis BacMam ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected with the NeonTM transfection kit (Invitrogen) according to the manufacturer’s description ...
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bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... HEK293 GnTI- cells were cultured in Freestyle 293 medium (Invitrogen) supplemented with 2% FBS on a shaker at 37 °C in the presence of 8% CO2 to a density of 3 × 106 cells per ml ...
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bioRxiv - Biochemistry 2024Quote: ... GKO HEK293 cells were maintained in DMEM (Gibco Cat# 11995065) containing 10 % FBS (Gibco Cat# 16000044) ...
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bioRxiv - Cell Biology 2024Quote: FlpIn CHO and HEK293 cell lines were purchased from Invitrogen and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 5% Foetal Bovine Serum (ThermoFisher Scientific (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were transfected using Lipofectamine 3000 (Invitrogen, Cat# L3000008) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Flp-In T-Rex HEK293 cell lines (Thermo Fisher Scientific) expressing mitochondrial-relocated golgin-BirA* fusions (from John Shin ...
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bioRxiv - Neuroscience 2023Quote: ... Stable HEK293 lines were generated by lipofection (Lipofectamine 2000, ThermoFisher) of ∼80% confluent cells with 2 µg of linearized expression plasmid overnight according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The amplified DNA was debranched by adding 10 μL of 5X S1 nuclease buffer and 2 μL S1 nuclease (200 U; Thermo Fisher Scientific Inc., Waltham, MA), mixed and incubated at 25°C for 30 minutes and then 70°C for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and glucosylated species were detected using the periodic acid-Schiff (PAS) method (Glycoprotein staining kit, Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: The isolated glycoproteins were dried in a vacuum centrifuge overnight (Savant™ SPD131DDA SpeedVac™ Concentrator, ThermoFisher). These were immobilized in an acrylamide gel and reduced and alkylated ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Microbiology 2019Quote: ... The MERS-CoV S EMC/2012 gene was codon-optimized for mammalian expression and cloned into pDNA3.1+ (Invitrogen) as described previously [39] ...
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bioRxiv - Biochemistry 2022Quote: ... an 18-mer DNA oligonucleotide containing the ETV5 promoter (GGTTATGAATGAAAAACC) and its reverse complement were purchased from ThermoFisher with HPLC purification ...
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bioRxiv - Microbiology 2020Quote: ... SARS2-S or MERS-S - C-terminally fused to the green fluorescence protein (GFP) - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Microbiology 2023Quote: ... of 78 of 15-mer peptides covering DBV GnH formulated at 0.625μg/ml for each peptide in RPMI medium (Gibco) in the 96-well plate ...
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bioRxiv - Microbiology 2021Quote: ... Binding studies in HEK 293T cells were performed using rVSV-SARS-CoV-2 Spike virions (rVSV/Spike) and binding was performed at room temperature in IMMULON 2HB flat bottom plates (Thermo Scientific, Waltham, MA).
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Microbiology 2021Quote: ... and probed with DNA oligonucleotide (Table S1) in OligoHyb buffer (Thermofisher) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Custom primers were purchased from Sigma and miRNA probes were from Life Technologies (Supplementary Methods, Table S1).
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bioRxiv - Neuroscience 2021Quote: ... three different linkers were synthesized (Table S1, GeneArt Invitrogen, codon optimized) and inserted into GCaMP8s ...
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bioRxiv - Cell Biology 2021Quote: ... S1 C (Pierce Unstained Protein MW Marker from Thermo Scientific, 26610) and Fig ...
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bioRxiv - Cell Biology 2021Quote: ... S1 D (PageRuler Plus Prestained Protein Ladder from Thermo Scientific, 26619). SDS-PAGE was performed for each septin prep.
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bioRxiv - Immunology 2022Quote: ... TaqMan probes and primers were purchased from Life Technologies (Table S1). Expression was normalized to Gapdh as well as expression levels of individual genes in a non-transplanted lymph node ...
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bioRxiv - Developmental Biology 2020Quote: ... and Pax7 using pre-validated primer sets (TaqMan, ThermoFisher; Table S1). 10 μl or 20 μl reaction volumes were prepared using TaqMan Fast Advanced Master Mix (ThermoFisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... using 10 U S1 nuclease (Thermo Fisher Scientific, Cat no. EN0321), 1 U snake venom phosphodiesterase (Exo-PI ...
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bioRxiv - Immunology 2021Quote: S1 protein was biotinylated according to manufacturer’s instructions (EZ-link, Thermofisher), and was conjugated to a U-Plex MSD linker (Mesoscale Diagnostics) ...
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bioRxiv - Microbiology 2020Quote: Dynabeads His-Tag Isolation & Pulldown (Invitrogen) were washed in PBS-TH (PBS ...
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bioRxiv - Immunology 2021Quote: ... V5 tag-152Sm (Thermo Fisher Scientific), anti-DYKDDDDK (FLAG ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-myc-tag (Invitrogen, R950-25), zn5 (Oregon Monoclonal Bank ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-V5 tag (Invitrogen, R96025), rabbit anti-ZNF598 (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: ... magnetic beads (Dynabeads His-Tag, Invitrogen) were coated with histidine tagged S protein according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Tandem Mass Tag (TMT, Thermo Scientific) labeling was performed on 330 μg of purified peptides from each sample as previously reported (Song et al. ...
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bioRxiv - Biochemistry 2022Quote: ... mouse monoclonal anti-his6 tag (Thermofisher) 1/10,000 dilution ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) was coated at 2 μg/mL in PBS onto half-area 96-well high binding plates (Corning ...