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Citations for Thermo Fisher :
351 - 400 of 4283 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Transformants were screened via colony PCR (Table S1, Fig. S1) using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific, USA) to confirm integration of donor DNA elements at the desired genomic locus and verified by sequencing ...
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bioRxiv - Immunology 2021Quote: ... The preparation of eVLPs expressing either Wuhan reference Spike or Beta variant Spike were formulated in Aluminum phosphate (Alum, Adjuphos®, Invitrogen) to obtain vaccine candidate VBI-2902a and VBI-2905a ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using D614G Spike and Delta Spike gene-specific primers and probes and TaqMan Fast Advanced Master mix (Cat# 4444963, Applied Biosystems) on a QuantStudio 5 system (Applied Biosystem) ...
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bioRxiv - Microbiology 2024Quote: ... To visualize spike in Western Blots we used anti SARS-CoV-2 Spike S2 (1A9) mouse monoclonal antibody (ThermoFisher, MA5-35946) at 1:1000 diluted in 3%BSA in TBS-T (0.01% Tween) ...
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bioRxiv - Microbiology 2022Quote: ... of the SARS coronavirus strain Tor2 (NC_004718.3) was ordered as a synthetic DNA string fragment (GeneArt synthesis; Thermo Fisher Scientific, Darmstadt, Germany) and cloned into the pEXPR103 expression vector (IBA Lifesciences ...
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bioRxiv - Immunology 2021Quote: ... and coronavirus RBDs were expressed and purified from Expi293F cells cultured in 33% Expi293 Expression / 66% FreeStyle Expression medium (Thermo Fisher Scientific) and grown in baffled polycarbonate shaking flasks (Triforest ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Microbiology 2022Quote: ... GISAID: EPI_ISL_1969270) and MERS (GenBank: AFS88936.1) were synthesized and cloned into pcDNA3.1 by GeneArt technology (Thermo Fisher Scientific GENEART GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... plus 10 % FCS (Gibco, Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1% FCS (Gibco). Followed by 3 days of culture ...
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bioRxiv - Bioengineering 2019Quote: ... FC from Life Technologies; and 2 ...
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bioRxiv - Neuroscience 2020Quote: ... containing 10% FCS (Invitrogen), and gentamycin (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... dialyzed FCS (Thermofisher, # 26400044), 28 μg/mL L-arginine-HCl (Arg0 or Arg10 ...
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bioRxiv - Biochemistry 2021Quote: ... including 10% FCS (Gibco), 2 mM glutamine (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... with 10 % FCS (Gibco), 2 mM glutamine (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... containing 10 % FCS (Gibco). Macrophages were seeded into 24-well plates at 105 cells per well.
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bioRxiv - Microbiology 2022Quote: ... containing 10% FCS (Gibco), 40 mM Hepes (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5% FCS (Gibco) in PBS with 0.01% Tween-20 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco, 26400044), 2.5% 1M HEPES buffer (Sigma ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... containing 10% FCS (Gibco), 1 mg/ml Collagenase VIII and 50 µg/ml DNase I (both Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10% FCS (Gibco). MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10% FCS (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... and 10% FCS (GIBCO). Cells were regularly tested for mycoplasma contamination.
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bioRxiv - Immunology 2022Quote: ... containing 10% FCS (Gibco) and 1% Penicillin/Streptomycin (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... with 5% FCS (Gibco), MEM only ...
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bioRxiv - Genetics 2023Quote: ... with 15% FCS (ThermoFisher), 0.1 mM beta-mercaptoethanol,100 U/ml penicillin and 100 mg/ml streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10% FCS (Gibco) for 10 days ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FCS (Invitrogen, 41966029), 10% penicillin/streptomycin at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... with 10 % FCS (Gibco) and 1 % PenStrep ...
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bioRxiv - Cell Biology 2020Quote: ... anti-V5 tag (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... V5 tag (R960-25, Invitrogen), GAPDH (sc-47724 ...
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bioRxiv - Cancer Biology 2022Quote: ... and HA tag (#26183, Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... V5-tag (Invitrogen 46-0705), STING (CST D2P2F or D1V5L) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-V5 tag (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... V5-tag (46-0705, Invitrogen)(1.12 ug antibody/500 ug protein IP ...
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bioRxiv - Immunology 2024Quote: ... anti-HA Tag (Invitrogen,26183), anti-Flag (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the spike protein was detected using anti-SARS-CoV Spike Protein (SDelta3) monoclonal antibody (7G12, Thermo Fisher Scientific, CA, USA)) raised against immunogen aa168-461 of the S1 domain.
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bioRxiv - Microbiology 2020Quote: ... after incubation of plugs with S1 nuclease (Thermo Fisher Scientific) according to the manufacturer recommendations ...
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bioRxiv - Physiology 2021Quote: ... and Ki67 (Thermo Fisher Scientific, RM-9106-S1, 1:100) were used for immunostaining ...
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bioRxiv - Cell Biology 2022Quote: ... HMT-3522 S1 cells were grown in DMEM (Gibco, #41966) 1:1 with Ham’s F12 nutrient mixture medium (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... the RNA sample was digested using nuclease S1 (EN0321, Thermofisher) and DNase I (EN0521 ...
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bioRxiv - Microbiology 2024Quote: ... the RNA sample was treated with nuclease S1 (EN0321, Thermofisher) according to the product instructions and resolved by agarose gel electrophoresis.
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bioRxiv - Cell Biology 2024Quote: ... transfected with 40nM siRNAs (Supplemental Table S1) using RNAiMAX (Invitrogen), incubated in growth media for 24h ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 S1 protein primary antibody (Thermo Fisher Scientific) at 1µg/ml were added for 1 h at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ERCC RNA Spike-In Mix (Thermo Fisher Scientific 4456740) was used in library preparation ...
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bioRxiv - Developmental Biology 2020Quote: ... containing the ERCC RNA spike-in Mix (Ambion, #4456740) diluted (1:100.000 ...
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bioRxiv - Molecular Biology 2021Quote: ... ERCC RNA Spike-in control mixes (Ambion, catalogue #4456740) were added to 1µg of total RNA according to manufacturer ...