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Citations for Thermo Fisher :
51 - 100 of 4283 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: HEK293 cells (Invitrogen) were grown in DMEM (1g/L glucose ...
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bioRxiv - Synthetic Biology 2022Quote: HEK293 cells (ThermoFisher; further authenticated by assessing cell morphology and growth rate ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2020Quote: ... spike protein was expressed by transiently transfecting plasmid encoding the HexaPro spike variant2 containing substitutions S383C and D985C3 with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Biochemistry 2021Quote: ... the anti-camel antibody-resin was vortexed vigorously and 300 ul was aliquoted into the wells of a 96 well plate (Nunc™ 96-Well Polypropylene DeepWell™ Storage Plates) ...
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bioRxiv - Developmental Biology 2022Quote: ... or with Pierce™ Glycoprotein Staining Kit (Thermo Scientific) after separation.
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bioRxiv - Microbiology 2020Quote: ... and S1 nuclease (Invitrogen). Total RNA was extracted from the pulverized samples using the TRIzol Plus RNA Purification Kit (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... S1 nuclease (ThermoFisher #18001016) at 20 U/ml in S1 nuclease buffer and 50 mM NaCl was added to one of the two slides and incubated for 30 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... and stained using SARS/SARS-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody (Invitrogen MA5-29981) as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... AON (13-mer) transfection was performed with Lipofectamine RNAiMAX (Invitrogen) in OptiMEM serum-reduced media (Gibco ...
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bioRxiv - Biochemistry 2022Quote: HEK293 and commercially available Flp-In T-REx HEK293 cells (Invitrogen; R78007) were cultured in DMEM (GIBCO ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells (ThermoFisher Scientific) were cultured in DMEM/F-12 ...
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bioRxiv - Bioengineering 2022Quote: ... FreeStyle HEK293 cells (ThermoFisher) were used for recombinant S protein production ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293 FT cells (ThermoFisher) were co-transfected with plasmids pCMV-dR8.2 dvpr (gag-pol ...
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bioRxiv - Synthetic Biology 2019Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293-FT (Invitrogen # R70007) cells were grown in Dulbecco’s Modified Eagle Medium (ThermoFisher #11965118 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 (Thermo Fisher Scientific) were cultured in DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Microbiology 2020Quote: The spike protein gene from MERS-CoV strain Camel/Qatar/2/2014 (GenBank accession number KJ650098.1) was codon optimized for humans and synthesized by GeneArt (Thermo Fisher Scientific). The synthesized S gene was cloned into a transgene expression plasmid comprising a modified human cytomegalovirus immediate early promoter (CMV promoter ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
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bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
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bioRxiv - Microbiology 2021Quote: ... capped 37-mer was digested using RNase T1 cleavage kit (Ambion). Cleavage reaction was performed for 15 min at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... ERCC spike-in (Invitrogen) were added in the lysis buffer (1:1000000 ...
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bioRxiv - Cancer Biology 2024Quote: ... ERCC spike-in (Invitrogen) was added at a ratio of 2uL of spike-in per 1ug of RNA.
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... Four peripheral blood samples from two Bactrian camel were collected as the source of lymphocytes using a lymphocyte isolation kit (Thermo Fisher Scientific). Total RNA was extracted ...
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bioRxiv - Molecular Biology 2021Quote: ... (2020; Figure S1; Life Technologies). The construct was cloned into pGEM®-T Easy (Promega Corp. ...
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bioRxiv - Microbiology 2021Quote: ... S1 Nuclease (Thermo Fisher Scientific) digestion was subsequently performed to de-branch chimeric DNA formed during the rolling circle amplification process ([5]) ...
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bioRxiv - Microbiology 2020Quote: ... S1 nuclease (ThermoFisher Scientific, EN0321) treatment was carried out after inactivation of protease with 0.56 u/µl nuclease in S1 nuclease buffer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 10 units S1 Nuclease (Invitrogen) in S1 Buffer (30 mM sodium acetate pH 4.6 ...
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bioRxiv - Cell Biology 2023Quote: ... S1 nuclease (Invitrogen cat # 18001016) was diluted 1/100 in S1 buffer and used at 20 U/ml ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2 ...
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bioRxiv - Biochemistry 2022Quote: HEK293 wild type (ATCC, #CRL-1573) and HEK293 Flp-in T-REx (ThermoFisher, #R78007) wild type cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 Flp-In T-REx cells (or HEK293 T-REx; Thermo Fisher Scientific, R78007) and HEK293 cells [American Type Culture Collection (ATCC) ...
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bioRxiv - Microbiology 2020Quote: ... once and then 100 μl of 1:300 diluted secondary antibody (ThermoFisher Cat # A-21091 for Fc-tag and ThermoFisher Cat # MA1-21315-647 for polyhistidine-tag ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were targeted directly using an anti-ALFA nanobody carrying a 5xR1 docking strand (5-TCCTCCTCCTCCTCCTCCT-3’) (Massive-TAG-Q Anti-ALFA Custom, Massive Photonics, GmbH) which was diluted in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293 Freestyle suspension cells (ThermoFisher), and immortalized human cord blood-derived mesenchymal stromal cells (cbMSCs ...
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bioRxiv - Neuroscience 2020Quote: HEK293-FT (RRID:CVCL_6911, Thermo Scientific) cells were grown in DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-FlpIn cells (ThermoFisher Scientific) were transfected with empty vector (negative control ...
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bioRxiv - Biochemistry 2022Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293-FT (Thermo Fisher Scientific) in DMEM with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293-F cells (ThermoFisher R79007) were transfected with the transfer plasmid and the packaging plasmids (Addgene plasmids #12263 and #8454 ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...