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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Mice were intraperitoneally injected with 5-ethynyl-2’-deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were pulsed with 1 mM 5-Ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 3 hr ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μL of 200mg/ml 5-ethynyl-2’-deoxyuridine (EdU; E10187, ThermoFisher Scientific) was administered daily for 2 weeks by intra perennial injections into 4 week ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Proteins were reduced with 5 mM tris (2-carboxyethyl) phosphine (Thermo Fisher Scientific) at 90 °C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: Mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 2 hours prior to splenocyte harvest ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were treated with 10 µM 5-ethynyl 2’-deoxyuridine (EdU) (Invitrogen) for 15 min to visualize replicated DNA ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were treated with 10 μM 5-ethynyl-2’-deoxyuridine EdU (#E10187, Invitrogen) 24 hours prior to harvest ...
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bioRxiv - Developmental Biology 2022Quote: ... larvae were incubated in 20 mM 5-ethynyl-2′-deoxyuridine (EdU) (Fisher Scientific) diluted in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 ml of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Biochemistry 2022Quote: ... cells were pulsed with 20 μM EdU (5-ethynyl-2′-deoxyuridine, Thermo Fisher) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... cells at 2×107 /mL with CTV at 5 μg/mL (Thermo Fisher) and incubating for 15 min at 37 °C followed by blocking in FBS and washing with complete medium according to the aforementioned protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 mL of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Cancer Biology 2023Quote: ... or 5- (and -6)-chloromethyl- 2′,7′-dichlorofluorescein diacetate (CM-H2DCFDA, ThermoFisher, C6827), or hydroxyphenyl fluorescein (HPF ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were incubated with 5 mM tris(2-carboxyethyl)phosphine (TCEP) (Thermo Scientific) in 50 mM ammonium bicarbonate for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated in medium containing 20 mM 5-ethynyl-2’-deoxyuridine (Invitrogen) and 1 mM 5-fluoro-2’-deoxyuridine (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... we use the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific A10044). Procyclic of Cas9 and LmexNAT10-/+ cell lines were collected at logarithmic growth phase and incubated with 100 µM 5-ethynyl-2’-deoxyuridine (EdU ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fisher Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 488 anti-mouse/human ɑ-tubulin (B-5-1-2; Invitrogen). Collagen gels were rinsed with PBS prior to imaging by confocal microscopy.
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bioRxiv - Neuroscience 2024Quote: ... mixed retinal cultures were loaded with 5 µM Fura-2 AM (Thermo Fisher) with 0.01% pluronic F-127 at 37°C for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... treated with 5 µl of TURBO DNase (2 U/µl, Thermo Fisher Scientific) on ice for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 mM tris(2-carboxymethyl)phosphine (Bond-BreakerTM TCEP, Thermo Fisher Scientific). In-solution digestion was performed overnight in a ThermoMixer at 2000 rpm at 37 °C with a protein-to enzyme ratio of 1 to 100 for both LysC (Wako ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079 ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated upon shaking at the growth temperature for 5 min with following concentrations: FM 5-95 (2 µg/ml; Thermo Fisher Scientific), DiSC3(5 ...
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bioRxiv - Cell Biology 2021Quote: ... The sgRNA sequences targeting CD33 exon 2 were 5’-TCCATAGCCAGGGCCCCTGT and 5’-GCATGTGACAGGTGAGGCAC.25 U937 cells were washed three times in PBS (Gibco 10010-023) and resuspended in complete Nucleofector Kit C (Lonza Biosciences VCA-1004 ...
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bioRxiv - Pathology 2020Quote: ... Aliquots of 15 μL per sample were loaded at a rate of 5 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) which was equilibrated with 98% Buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at 37°C (with 5% CO2 and humidity) before indicator loading with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA (2-5 µg) was treated with DNAse I (Thermo Fisher Scientific, Cat # AM1906) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were incubated with 10 µM 5-ethynyl-2’ deoxyuridine (EdU, Thermo Fisher) for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were then blocked with 2% BSA and 5% donkey serum (ThermoFisher Cat # NC9624464) in PBS for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: Click-iT EdU (5-ethynyl-2’-deoxyuridine, a thymidine analog) kit from Life Technologies was used to perform DNA replication assay (Milton et al. ...
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bioRxiv - Genomics 2020Quote: ... 5 µl of 10X Turbo DNase buffer and 2 µl Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... The samples were then reduced with 5 mM Tris(2-carboxyethyl)phosphine (ThermoFisher Scientific) for 30 minutes at 37 °C and then alkylated in the dark for 30 minutes at 25 °C with 40 mM iodoacetamide (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5 μl of purified products were loaded on 2% E-Gel EX (Invitrogen) to verify the reaction performance and amplicon size ...
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bioRxiv - Neuroscience 2021Quote: ... and two injections of 5-ethynyl-2’-deoxyuridine (EdU: 0.01 mg/kg, Invitrogen #A10044), made as a 10 mM stock solution in DMSO and diluted for injection to 40% strength in 0.9% sterile saline ...
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bioRxiv - Developmental Biology 2022Quote: ... 5% mercapto-2-ethanol and 1X Halt Protease Inhibitor Cocktail (Thermo Scientific; 1:200). Proteins were separated using SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... and were supplemented with 10mM EdU (5-ethyl-2’-deoxyuridine) (ThermoFisher Scientific, Rockford, IL) during the final 6 hours of incubation to mark proliferating cells ...
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bioRxiv - Molecular Biology 2022Quote: ... mixed with 5 μl of 2 x Novex TBE-Urea sample buffer (ThermoFisher Scientific) and denatured for 3 minutes at 70°C ...
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bioRxiv - Molecular Biology 2022Quote: ... For labeling of proliferating cell populations with 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific), animals received IP injections of EdU (50 mg/kg ...
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bioRxiv - Biophysics 2022Quote: ... 5 μM Fluo-2 Low Affinity (TefLabs) and 2.5 μM Alexa568 (Invitrogen, Waltham MA) as previously described (36) ...