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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and CYPBΔPSI (forward 5′ CGAACTTGTGTTTGGTGGTGTT-3′ and reverse 5′-GCCTTTTTCAGTCACCGGAACA-3′) primers on a StepOnePlusTM Real-Time PCR System (Applied Biosystems, USA) as previously described (Muthusamy et al ...
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bioRxiv - Microbiology 2020Quote: ... After 24 hours (24-32 hpi) parasite DNA was stained with SYBR Green DNA stain (1: 10,000) (Invitrogen #S7563) for 30 min at 37 °C and the fluorescence intensity was measured using a MACS Quant Analyzer 10 (at least 200,000 RBCs were measured per sample ...
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bioRxiv - Cancer Biology 2021Quote: ... 5×104 cells per well were seeded in 24-well Falcon plates (Fisher Scientific, Cat #353047). 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... After 24 h incubation at 37°C in a 5% CO2 chamber (Cytoperm 2, ThermoFisher Scientific), the culture supernatants were collected and stored at –20°C until further analysis.
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bioRxiv - Cancer Biology 2021Quote: ... clone IMAGE ID 4977050 was obtained from Source Bioscience and PCR cloned using oligos (Tet2fwd: 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAatgccaaatggcagtacagt-3’ and Tet2rev: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTtcatacaaatgtgttgtaag-3’) into pDonor221 (Invitrogen Gateway, ThermoFisher) and sequenced ...
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bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer containing the Sp6 promoter (5’ ATTTAGGTGACACTATAGCCTCTTCAGTTCCTTCTTCCATC-3’).The aqp1a.1 probe was generated from whole extracted cDNA at 30hpf and amplified with the primers (5’-GTCATGAACGAGCTGAAGAGC-3’) and (5’-GGGTCACTTTGAGGACATCTC-3’),incorporated into the pCR-BluntII-TOPO vector (Invitrogen, 45-0245) (containing both the SP6 and T7 promoters) ...
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bioRxiv - Genomics 2020Quote: ... The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5’-AGA GTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTTC-3’) and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul ...
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bioRxiv - Microbiology 2020Quote: ... 229E-RP reverse primer (5’-CCAACACGGTTGTGACAGTGA-3’) and the TaqMan minor groove binder (MGB) probe 229E-TP (FAM-5’-TCCTGAGGTCAATGCA-3’-NFQ-MGB; Thermo Fisher Scientific), derived from the HCoV 229E membrane protein gene sequence as described previously (Vijgen et al. ...
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bioRxiv - Microbiology 2021Quote: The mcr-1 gene was amplified from the clinical isolate ESBL20150072 (European Nucleotide Archive, accession number; SAMEA104060671) using primers: 5’-TTTTGGATCCCGGTTTTCGGGCTTTTTA-3’ and 5’-ATATGTCGACTCAGCGGATGAATGCGGT-3’ and Phusion polymerase (Thermo Fisher Scientific). PCR product was digested with BamHI and SalI and ligated into pACYC184 digested with the same enzymes (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
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bioRxiv - Genetics 2022Quote: ... 5’-GGCAAGCUGACCCUGAAGUdTdT-3’ / 5’-ACUUCAGGGUCAGCUUGCCdTdT-3’ or with a hsa-miR-34a mimic duplex: 5’-P-UGGCAGUGUCUUAGCUGGUUGUU-3’ / 5’-P-CAAUCAGCAAGUAUACUGCCCUA-3’ according to the protocol for Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... a fragment containing 2.21 kb upstream regulatory region of ELF3 was amplified from Col-0 genomic DNA using primers ELF3Prom-Fw (5’-CACCCTTATAAATAAAATTCC-3’) ELF3Prom-Rv (5’-CACTCACAATTCACAACCTTTTTC-3’) and cloned into the Gateway System (pENTR™ Directional TOPO® Cloning kit, Invitrogen) to obtain the pELF3-TOPO plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... Drosophila dSF2 sequence was PCR amplified (dSF2 F 5’-CACCATGGGATCACGCAACGAGTGCCG-3’ and dSF2 R 5’- ATAGTTAGAACGTGAGCGAGACCTGG-3’) was cloned into pEntry-TOPO vector (Thermo Fisher Scientific). The pENTR-dSF2 vector was recombined with Gateway plasmid pTWH (Drosophila Genomics Resource Center ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Microbiology 2022Quote: ... The 16S rRNA gene was amplified with the primers 8f (5′-AGAGTTTGATCCTGGCTCAG-3′; Weisburg et al. 1991) and 1520 r (5′-AAGGAGGTGATCCAGCCGCA-3′; Edwards et al. 1989) (Invitrogen, CA, USA). A volume of 0.5 μL DNA extract was used for 50 μL PCR reactions containing 2 units Taq DNA polymerase ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression of Kdm5c was detected us-ing the primers 5’-CCCATGGAGGCCAGAGAATAAG-3’ 5’-CTCAGCGGATAAGAGAATTTGCTAC-3’ and nor-malized to TBP using the primers 5’-TTCAGAGGATGCTCTAGGGAAGA-3’ 5’-CTGTGGAGTAAGTCCTGTGCC-3’ with the Power SYBR™ Green PCR Master Mix (ThermoFisher #4367659).
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bioRxiv - Cancer Biology 2024Quote: ... sequence is 5’-GCCGACCAAUUCACGGCCG-3’ and siRNA directed against GNA11 (siGNA11) is 5’-AAAGGGUACUCGAUGAUGC-3’) using Lipofectamine™ RNAiMAX (13778150, Fisher Scientific) and harvested 48 h post-transfection.
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bioRxiv - Microbiology 2023Quote: ... A549 cells were seeded in 96-well microtiter plates at a concentration of 5−104 cells per well for 24 h (37°C, 5% CO2) in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... GP2-293 cells were seeded at 5×106 on 10-cm plates and transfected 24 h later with 5 µg of LUJV-flag/LUJV-flag-mut and 5 µg Luciferase using Lipofectamine 2000 (Invitrogen). Cells’ media were replaced 5 h later to full medium ...
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bioRxiv - Microbiology 2021Quote: ... 5 μM of 20-base model RNA (5’-AAUCUAUAAUAGCAUUAUCC-3’; ThermoFisher Scientific) was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589 ...
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bioRxiv - Microbiology 2023Quote: ... 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated into a PCR by the use of a DreamTaqTM DNA Polymerase (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2020Quote: ... and Y14 siRNA (5’-GGGUAUACUCUAGUUGAAUUUCAUAUUCAACUAGAG-3’) with Lipo2000 (Invitrogen) in Optimem (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... siDDX42-4: 5’-AUCUCGAAUACCCUUUACG-3’ (ID:136410, Ambion®).
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bioRxiv - Cancer Biology 2020Quote: ... 5’-UGGUUUACAUGUCGACUAA-3’ KIF18A (Silencer Select s37882 – Ambion)8 5’-UCUCGAUUCUGGAACAAGCAG-3’ RAD51 (Silencer Select s11735 – Ambion) 5’-UGAUUAGUGAUUACCACUGCT-3’ RRM1 (On-Target plus SMARTpool – Dharmacon)
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bioRxiv - Genetics 2024Quote: ... 5′-UUAAUUUACGCGGUUUUUAUU-3′) using the RNAiMAX transfection reagent (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... BV2 cells were seeded in 24 well-plates at 70-90% confluence 24 hours prior to the experiment in high glucose DMEM (Gibco) supplemented with 10% FBS without antibiotics ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of DOT1B-Myc-BirA* was induced by addition of 5 ng/ml tetracycline for 24 h and cells were further incubated for 24 h with 50 µM D-biotin (Invitrogen) and 5 ng/ml tetracycline ...
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bioRxiv - Biochemistry 2020Quote: ... The aqueous supernatant was transferred to a new 2 ml screw-top tube to which 400 μl of 24:24:1 phenol:chloroform:isoamyl alcohol (Ambion cat. # 9732) was added ...
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bioRxiv - Biophysics 2021Quote: ... cells were plated into 24 well plastic culture dishes and transfected 24 hours later with Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Fibroblasts were plated at a density of 20,000 cells/well in a 24 well plate and fixed after 24 h in 4% paraformaldehyde (PFA, 28908, Thermo Scientific) for 10 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2024Quote: ... 100 μl of DPBS was added on top of the samples and 24 x 24 mm coverslips (Epredia Menzel Microscope Coverslips) were mounted on top using vacuum grease (ThermoFisher). Samples were stored at 4 °C if not immediately imaged.
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bioRxiv - Cell Biology 2022Quote: ... TERT (ThermoFisher #Bt03239211), and CDK4 (ThermoFisher #Bt03231354) ...
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bioRxiv - Microbiology 2020Quote: ... on a Fast Prep-24 instrument (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... using a Bead Mill 24 homogenizer (Thermo Fisher), two times at 4.5 m/s for 30 s ...
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bioRxiv - Bioengineering 2022Quote: ... and culturing in 24-well plates (Polystyrene, Nunc, Non-Treated Multidishes ...
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bioRxiv - Neuroscience 2020Quote: 24 well culture plate non-treated (Nunc, #144530)
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bioRxiv - Bioengineering 2021Quote: ... and cultured in 24-well plates (Polystyrene, Nunc, Non-Treated Multidishes ...
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bioRxiv - Neuroscience 2020Quote: ... and standard coverslips (24 × 50 mm2; Fisher Scientific). Slides were dried at RT for 24 hrs before storage at 4°C for > 24 hrs before imaging ...
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bioRxiv - Microbiology 2020Quote: Samples were incubated in 24-well plates (Nunc, ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... and standard coverslips (24 × 50 mm2; Fisher Scientific). Slides were dried at RT for 24 hrs before storage at 4°C for >24 hrs before imaging.
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bioRxiv - Molecular Biology 2021Quote: ... and 24 h in Trizol (ThermoFisher Scientific, 15596026). RNA was extracted from the aqueous phase on silica columns (Zymo ...
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bioRxiv - Biochemistry 2021Quote: Glass coverslips (60 × 24 mm; Thermo Fisher Scientific) were first cleaned by sonication in detergent for 60 min ...
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bioRxiv - Cell Biology 2021Quote: ... in a 24-well plate (Thermo Scientific, 142475) in neurobasal medium (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... 24 × 40 mm microscope cover glass (Thermo Scientific). First ...
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bioRxiv - Neuroscience 2023Quote: ... with cover glass (Fisher Scientific 24×50-1.5) using elvanol containing antifade (polyvinyl alcohol ...
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bioRxiv - Neuroscience 2023Quote: ... and cover-slipped (24×50 mm, Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... coated 24-mm glass coverslips (Thermo Fisher Menzel) and maintained at 37 °C ...