Labshake search
Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
-
bioRxiv - Microbiology 2021Quote: Nucleic acids were extracted from purified virus samples either using the PureLink Viral RNA/DNA Mini Kit (Thermo Fisher Scientific) or by phenol/ether method combined with ethanol precipitation ...
-
bioRxiv - Microbiology 2021Quote: ... VeroE6 cells were used for live virus titration of biological samples and were maintained in DMEM (#11965092, Thermo Scientific, USA) with 10% FBS.
-
bioRxiv - Microbiology 2021Quote: ... Five μl RNA (150 µl elution volume) was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
-
bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
-
bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
-
bioRxiv - Cell Biology 2022Quote: ... 4T1 cells were transduced with cell-free virus-containing supernatants for 48 hours and selected with 100 µg/mL of neomycin (Gibco) for 96 hours.
-
bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
-
bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
-
bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
-
bioRxiv - Cell Biology 2022Quote: ... Assays were performed in 24-well plates coated with Geltrex LDEV (lactose dehydrogenase elevating virus)-Free Reduced Growth Factor Basement Membrane Matrix (ThermoFisher) at 75 µl/cm2 according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... The resuspended virus was purified using a 30% (wt/vol) sucrose cushion at 100,000 × g (Sorvall Discovery 90SE, Thermo Fisher) for two- and-a-half hours at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
-
bioRxiv - Neuroscience 2021Quote: ... 500 ng of RNA was reversely transcribed using random primers (Eurofins) and Moloney murine leukaemia virus reverse transcriptase (M-MLV, Invitrogen) according to manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2021Quote: Heathy human donor PBMCs or skin fibroblasts were reprogrammed into iPSCs using the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Low passage primary fibroblasts were used to increase reprogramming efficiency ...
-
bioRxiv - Microbiology 2022Quote: ... The presence of engineered mutations was confirmed by Sanger sequencing following extraction of RNA from virus stocks with TRIzol (Invitrogen) and cDNA amplification using a OneStep RT-PCR Kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
-
bioRxiv - Microbiology 2019Quote: vRNA was extracted from virus supernatant containing the same amount of PFU using PureLink Viral RNA/DNA Mini Kit (Invitrogen). vRNA was reverse transcribed with Uni12/13 specific primers using Superscript IV First-Strand Synthesis System (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: ... filled with the infusate (virus particles suspended in a solution of phosphate-buffered saline (PBS) and Oregon Green 488 (Invitrogen)) into the centers of the craniotomies ...
-
bioRxiv - Molecular Biology 2019Quote: iPSCs were generated by transducing control and FXS patient fibroblasts with Sendai virus from the Cytotune 2.0 Reprogramming Kit (Life Technologies) per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2019Quote: Fibroblasts isolated either from human fetal liver tissues or human adult skin after collagenase dissociation were reprogrammed into iPSCs with the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Fibroblasts were used at low population doubling (less than 5 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed to convert RNA into cDNA using the Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Thermo Fisher) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... RNA samples were reverse transcribed (1µg of total RNA/sample) using the Moloney Murine Leukemia Virus (MML-V) enzyme (Life Technologies) and Oligo-dT Primers ...
-
bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
-
bioRxiv - Microbiology 2021Quote: ... that co-localises with IBDV virus factories (16) and a goat anti-mouse secondary antibody conjugated to Alexa-Fluor 488 (Invitrogen). Wells were scored as positive or negative based on the presence of infected cells ...
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed as previously described [39] using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
-
bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
-
bioRxiv - Cell Biology 2021Quote: ... 1µg was used for cDNA synthesis using a Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (SuperScript II from ThermoFisher Scientific) and random primers ...
-
bioRxiv - Microbiology 2019Quote: ... 200 ng of RNA from each sample was used to generate virus cDNA using in a Maxima H minus strand kit (Invitrogen) and ZIKV specific primers (Supplemental Table 6) ...
-
bioRxiv - Immunology 2021Quote: ... dilutions of IgG were co-incubated with the virus (1000-2000 TCID50) for 1 h at 37°C prior to addition of VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
-
bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
-
bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
-
bioRxiv - Bioengineering 2021Quote: ... 21] or a Sendai virus reprogramming kit (Cyto Tune 2.0 Sendai reprogramming vectors, Ref#A16517; Lot#A16517, Invitrogen, Carlsbad, CA). hiPSCs were cultured on hESC-qualified Matrigel® (Corning ...
-
bioRxiv - Microbiology 2021Quote: ... The attachment of HIV-1 virus produced in the presence of TMEM123 was also separately tested by pre-incubating the virus with mouse monoclonal anti-TMEM123 antibody (297617) (ThermoFisher) (25ug/ml ...
-
bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
-
bioRxiv - Cancer Biology 2022Quote: ... All cell lines were validated free of virus mycoplasma contamination using the MycoSEQ assay (Thermo Fisher Scientific, Waltham, MA, USA) or STAT-Myco assay (IDEXX Bioanalytics) ...
-
bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA isolation from the AAV9 virus injected region of the left frontal cortex was performed as previously described [40] by TRIzol LS Reagent (10296010, Invitrogen). For cDNA synthesis ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Virus propagation and titration was performed in LLC-MK2 cells using Opti-MEM supplemented with 1.6% TrypLE™ Express (Gibco) and 100 μg/ml CaCl2 (OptiMEMinf ...
-
bioRxiv - Plant Biology 2022Quote: ... was transcribed following DNase I treatment using Moloney Murine Leukemia Virus reverse transcriptase and an oligo dT(18) primer according to the manufacturer’s protocol (ThermoFisher Scientific). qPCR analysis was carried out in a CFX96-C1000 96-well plate thermocycler (Bio-Rad ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 µg of total RNA was subjected to random reverse transcription using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT; Invitrogen) and random primers (Roche) ...
-
bioRxiv - Cell Biology 2024Quote: ... GH3 cells were transduced with supernatants containing virus carrying the shMeCP2 or shhnRNP L construct using the Lipofectamine 3000 reagent (Invitrogen). After 48 h ...
-
bioRxiv - Neuroscience 2024Quote: ... PBMCs were reprogrammed into iPS cells using transient overexpression of pluripotency-associated factors (OCT4, KLF4, and SOX2) via non-integrating Sendai virus particles (Cytotune 2.0 kit, Life Technologies). At least five clones per TSC2+/+ and TSC2+/− donor were established into iPS cell lines ...
-
bioRxiv - Genetics 2024Quote: ... The iPSCs were made Sendai virus free by keeping in culture and testing for the presence of Sendai Virus (SeV) at different times by measuring SeV levels in total RNA using RT-qPCR with FAM labeled SeV (Thermofisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III, ThermoFisher Scientific). The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The virus concentration using Lenti-X Concentrator was performed with the manufacturer’s protocol and dissolved in Opti-MEM (Gibco #31985062) to yield a 10- or 15-fold concentration ...
-
bioRxiv - Microbiology 2023Quote: ... generated by Knut Falk in the late 1990s) or mouse IgG2a targeting influenza virus NP (clone D67J, Thermo Fisher Scientific) (both ...
-
bioRxiv - Developmental Biology 2023Quote: ... PD2 and TD1 hiPSC were generated from deidentified commercially available human peripheral blood mononuclear cell and tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (ThermoFisher, A16517). Every other month ...