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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... OD600 measurement in a microplate reader (Multiskan™ FC, Thermo Scientific, Etten-Leur, the Netherlands) where performed ...
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bioRxiv - Microbiology 2023Quote: ... Optical density was measured at 450 nanometer using a Multiskan FC machine (Thermo Scientific, USA) in combination with Mikrowin (software version 5.09 ...
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bioRxiv - Cancer Biology 2023Quote: Hap1 cells (Horizon Discovery) were maintained in IMDM supplemented with GlutaMAX and 10% FCS (Gibco). Cells regularly tested negative for mycoplasma contamination.
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bioRxiv - Immunology 2023Quote: ... Pheonix cell and LS174T cells were cultured in complete DMEM (Gibco, 10% FCS, 2mM GlutaMAX).
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bioRxiv - Immunology 2023Quote: ... Samples were then incubated with an Fc receptor binding inhibitor polyclonal antibody (Thermo Fisher Scientific), before staining with a surface antibody cocktail (Supplementary Table 1) ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Alexa Fluor 488 Anti-alpha 1 Sodium Potassium ATPase antibody (ab197496) ...
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bioRxiv - Immunology 2024Quote: ... followed by Fc-Block step with anti-mo CD16/CD32 (Invitrogen, Cat# 14-01061-86) and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD ...
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bioRxiv - Plant Biology 2024Quote: ... and reading absorbance at 620 nm in a photometer (Multiskan FC, Thermo Scientific, United States) and using a glucose standard curve to compare the colorimetric response of the samples ...
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bioRxiv - Immunology 2024Quote: ... followed by absorbance measurements at 450nm using a Multiskan FC Microplate Photometer (51119000; Thermo Scientific). As a positive control ...
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bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies with HRP (ab97250 ...
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bioRxiv - Immunology 2024Quote: ... Fc receptors were then blocked with anti-mouse CD16/CD32 (Invitrogen, Cat# 14-01061-86) along with extracellular staining with a mixture of conjugated antibodies in FACS buffer (1:250 for LP cells ...
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bioRxiv - Immunology 2024Quote: ... Absorbance was detected at 450 nm by the Multiscan FC (Thermo Fisher, Waltham, MA, USA).
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bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) horseradish peroxidase (HRP) was purchased from Invitrogen and 3,3″,5,5″-Tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2024Quote: ... + 10% FCS + 1% GlutaMAX + penicillin-streptomycin (P/S, 12.8 U/mL, Gibco, cat.15140-122) supplemented with 10% DMSO at -150°C ...
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bioRxiv - Immunology 2024Quote: ... containing 10% FCS (Biochrome, Cat. S0615-500ML) and 1% Penicillin/ Streptomycin (Thermo Fisher, Cat. 7001592). After sorting ...
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bioRxiv - Microbiology 2019Quote: The δ13C values of methane and CO2 in the headspace as well as of DIC in the slurries were determined using a Thermo Finnigan Trace GC connected to a DELTA Plus XP IRMS (Thermo Scientific, Bremen, Germany) as described previously [35] ...
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bioRxiv - Systems Biology 2019Quote: ... and a vendor supplied high resolution (HR)-MS mass spectral library for the GC-MS dataset using proprietary TraceFinder™ software (Thermo Fisher Scientific) and MS-DIAL software ver ...
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bioRxiv - Cell Biology 2020Quote: ... Organic acids were quantified using methods described previously (Jensen et al., 2006) employing Trace Ultra GC coupled to ISQ MS operating under Xcalibur 2.2 (Thermo Fisher Scientific, Austin, TX). Metabolite levels were normalized to total protein as determined by BCA (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... blanks and internal standards were analyzed by gas chromatography (GC) coupled to an APPI-Q-Exactive HF mass spectrometer (Thermo Fisher Scientific, USA). GC was equipped with a DB-5 column (30 m x 0.25 mm i.d. ...
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bioRxiv - Immunology 2021Quote: ... Amplicons were generated using 100 ng DNA as input in a final volume of 40 μL (consisting of 8 μl 5x Phusion GC buffer (Phusion High-Fidelity DNA Polymerase set (ThermoFisher Scientific, Cat#F534), 0.8 μL dNTPs (dNTP Set ...
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bioRxiv - Microbiology 2022Quote: ... to molten Gonococci agar (GC agar) before pouring rectangular assay plates with the same dimensions as the standard 96-well microplates (Thermo Scientific Nunc # 267060). The GC agar recommended for N ...
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bioRxiv - Microbiology 2024Quote: ... The 13C/12C isotope ratios of methane and carbon dioxide in headspace were determined using GC - IRMS (isotope ratio mass spectroscopy, Thermo Fisher Scientific, Germany).
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bioRxiv - Microbiology 2023Quote: ... ethene and acetylene were detected by gas chromatography combined with mass spectrometry (GC-MS) with an Rt®-Q-BAND column (Retek, PA, USA) and DSQ-MS (Thermo Fisher Scientific). Hydrogen gas was detected by Compact GC (Global Analyzer Solutions ...
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bioRxiv - Microbiology 2023Quote: ... and cDCE were measured by Gas chromatography and mass spectroscopy (GC-MS) installed with an Rt®-Q-BOND column (Retek, PA, USA) and a DSQ MS (Thermo Fisher Scientific). Hydrogen and methane were detected by compact GC (Global Analyzer Solutions ...
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bioRxiv - Synthetic Biology 2024Quote: ... The organic phase samples were analyzed by gas chromatography (GC) with flame-ionization detection (FID) in a TraceTM 1300 gas chromatograph (Thermo Fisher Scientific Co.) [87–89] ...
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bioRxiv - Microbiology 2020Quote: Concentrated live or inactivated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermofisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Neuroscience 2020Quote: ... patient-derived fibroblasts were infected with Sendai virus using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen, CA, US) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... RNA was reverse transcribed and amplified using TaqMan Fast Virus 1-Step Master Mix qRT-PCR Master Mix (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Virus titres were measured from a thawed aliquot by: (1) mixing 20 μl with 200 μl of TRIzol (Invitrogen); (2 ...
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bioRxiv - Neuroscience 2019Quote: ... PBMCs were transduced with 2.0 Sendai virus particles containing four Yamanaka factors using the CytoTune-iPS Sendai Reprogramming Kit (ThermoFisher). After seven days ...
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bioRxiv - Microbiology 2019Quote: ... Initial virus was recovered from T7 transcribed RNA using reverse transfection of Huh7.5.1 cells by Lipofectamine® 2000 (Invitrogen) (for virus rescue ...
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bioRxiv - Microbiology 2019Quote: ... Two microliters of extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol and primers specific for HCoV-229E [30] or RSV-B [31] ...
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bioRxiv - Microbiology 2021Quote: ... Virus prepared for purification by ultracentrifugation was grown in serum-free media (VP-SFM; Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Virus prepared for purification by ultracentrifugation was grown in serum-free media (VP-SFM; Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... a homolog of the vaccinia virus H3L gene) [13] using the Path-ID™ qPCR Master Mix (Thermofisher Scientific). Nucleic acid from capripoxvirus positive samples was further analysed by a qPCR targeting LSDV011 designed to differentiate the three capripoxvirus species [14] and a qPCR targeting LSDV008 designed to differentiate LSDV wildtype strains from Neethling vaccine strains [15] ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed with Taq-Man™ Fast virus 1-step master mix (ThermoFisher Scientific, Cat. 4444436) and primer-probe sets targeting SARS-CoV-2 nucleocapsid (N1 set ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were generated by transfecting the clone into Vero cells using Lipofectamine 2000 (Thermo Fisher, cat# 11668-019), and harvested at various times post-transfection.
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bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... virion concentration was determined indirectly through the DNA absorbance at 260/280nm of the virus stock (NanoDrop, Thermo Scientific). The MOI equivalent (MOI eq. ...
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bioRxiv - Cancer Biology 2020Quote: ... converted to cDNA by RT with random hexamers and Moloney murine leukemia virus (MuLV) reverse transcriptase (Thermo Fisher Scientific) and used in PCR with the following primers ...
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bioRxiv - Microbiology 2021Quote: 400,000 CEM-SS or CEM-T4 cells were infected with 1.6 ng of p24-CA of wildtype or mutant NL4-3 virus in serum-free RPMI medium (Invitrogen) at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... A high-titer virus stock for hPolεCD(insect) was obtained by using the Bac-to-Bac Baculovirus Expression System from Invitrogen. 1.8×109 Sf21 cells in 1 L shaking culture were infected with the recombinant virus at a multiplicity of infection of 2 and cultivated at 27 ºC for 56 hr ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant P0 virus for each gene were generated in ExpiSf9 insect cells following the manufacturer’s recommendations (Thermo Fisher). The amount of each virus needed for infection has been optimized for maximal production of the two proteins ...
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bioRxiv - Microbiology 2020Quote: ... Two microliters of extracted RNA were amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
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bioRxiv - Microbiology 2020Quote: ... one for virus quantitation (TCID50) and the other for vRNA extraction in TRIzol (ThermoFisher, 15596026; 1 in 3 dilution). Samples were stored at -80°C until required ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed according to the manufacturer’s instructions using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and a OneStepPlus Real-Time PCR System (96-well format ...