Labshake search
Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
-
bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
-
bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
-
bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
-
bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
-
bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
-
bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
-
bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
-
bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
-
bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... Bone marrow human macrophage (KG-1 ATCC® CCL246™) passages 2–5 were cultured in cell suspension flasks using IMDM medium (Gibco™ GlutaMAX™, HEPES) with 20% FBS ...
-
bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
-
bioRxiv - Molecular Biology 2021Quote: ... anti-afadin (clone D1Y3Z) or Alexa Fluor 488 anti-DYKDDDDK (flag) tag monoclonal antibody (L5, Life Technologies). The plasma membrane was stained with CF594 dye conjugated wheat germ agglutinin (Biotium) ...
-
bioRxiv - Cell Biology 2019Quote: ... and pENTR-Megator constructs with pHWG (blasticidinR; C-terminal EGFP tag) using Gateway LR Clonase II (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysates were immunoprecipitated by incubating with an antibody to the c-Myc tag (Invitrogen 13-2500) on BBS2 as described above and immunoblotted with the c-Myc antibody for BBS2 and with the anti-Flag antibody (Sigma F3165 ...
-
bioRxiv - Neuroscience 2020Quote: ... Tandem Mass Tags (TMT 10-plex) kits and microBCA protein assay kit were purchased from Thermo Fisher Scientific (Rockford ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were stained with anti-GFP antibody (5 μg/ml, Thermo Fisher, RFP Tag Monoclonal Antibody RF5R) and whole node confocal scans were used to quantify percent metastatic area of total node via image thresholding in ImageJ ...
-
bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
-
bioRxiv - Biophysics 2020Quote: ... the kdpFABC operon with the 8x histidine tag was cloned into the pBAD vector (Invitrogen, Carlsbad CA) and introduced into E ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pierce Trypsin/Lys-C Protease Mix and tandem mass tag (TMT)-labeling kit were from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were purified via affinity pulldown of their StrepII tags using a StrepTrap HP column (Fisher Scientific) and eluted into Buffer A supplemented with 2.5mM desthiobiotin (Fisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
-
bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tandem Mass Tag™ 6-plex (TMTsixplex™) reagents kits for isotopic labeling were from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Microbiology 2022Quote: ... Desalted peptide samples from each sample were labeled with 10-plex tandem mass tags (TMT, ThermoFisher Scientific) with one of the TMT channels (131 ...
-
bioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) eleven plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
-
bioRxiv - Physiology 2022Quote: ... 25 μg of peptides per sample were labeled with isobaric tandem mass tags (TMT10plex, Thermo Fisher Scientific) as described previously [48] ...
-
bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... The ATP2 proteins fused to the BAD tag were detected with the Avidin HRP-conjugate probe (Invitrogen), diluted to 1:20 000 in 5 % (w/v ...
-
bioRxiv - Molecular Biology 2019Quote: ... and delivered into a modified pEG containing 3XFLAG and 9XMYC tags using the LR reaction kit (Invitrogen). The pEG destination vectors were transformed into Agrobacterium strain AGL0 ...
-
bioRxiv - Molecular Biology 2020Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and pooled ...
-
bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
-
bioRxiv - Systems Biology 2020Quote: ... 250 μg of peptide were labeled using TMT11Plex Mass Tag Labeling Kit (Thermo Scientific, Lot number UE283355) following the manufacturer’s instructions and they were combined as shown in Figure 1 ...
-
bioRxiv - Microbiology 2020Quote: Proteins with C-terminal biotin tags were attached to streptavidin-coated dynabeads (Dynabead M-280 Streptavidin, Invitrogen) following standard procedures ...
-
bioRxiv - Biochemistry 2021Quote: ... Desalted peptides were labeled using a Tandem Mass Tag 11-plex isobaric label reagent set (ThermoFisher #A34808) with the following setup ...
-
bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
-
bioRxiv - Immunology 2020Quote: ... the polyhistidine tag of 6xHis-TEV-dreC5a was removed by incubation with TEV protease (Thermo Fisher Scientific) and the protein was FITC-labelled at the N-terminus using the sortagging method (37) ...
-
bioRxiv - Microbiology 2019Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled ...
-
bioRxiv - Physiology 2020Quote: ... 25 μg of peptides were labeled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific) as described previously (34) ...
-
bioRxiv - Biochemistry 2021Quote: ... as described in (62).The 6xHA with linker and mCitrine tags were cloned into pDONR-P2RP3 (Invitrogen) as described in de Saint Germain et al ...
-
bioRxiv - Cell Biology 2021Quote: The LAP tag from pIC113 vector (Cheeseman and Desai, 2005) was subcloned into pcDNA5.0/FRT vector (Invitrogen) to make the pcDNA5.0/FRT-LAP-N vector ...
-
bioRxiv - Biochemistry 2021Quote: ... followed by a short linker FLAG-epitope tag and C-terminal Histidine6 was synthesised by GeneArt (Invitrogen). To generate a FLAG-free HuACE2 fusion ...
-
An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) 6-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
-
bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...