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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: BmN cells infected with WT virus at indicated time were harvested and total RNA was extracted by using TRIzol reagent (Invitrogen). 2 μg total RNA was prepared to synthesis the cDNA by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen) ...
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bioRxiv - Microbiology 2023Quote: ... The following day we washed cells with DPBS twice and added half-log10 dilutions of the virus sample in infection media (MEM medium (Gibco) containing 0.3% BSA (fraction V ...
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bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Lungs from K18-ACE2 C57BL/6 mice infected with virus were collected at indicated time points and were homogenized in Trizol (Invitrogen) and RNA was isolated using manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Total RNA was extracted from clinical specimens (swabs and tissues) and virus isolates using the MagMax AM1836 96 Viral RNA Isolation Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT) (Invitrogen).
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
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bioRxiv - Biochemistry 2023Quote: ... Concentrated virus was applied through a 0.45 μm surfactant free cellulose acetate syringe filter (Nalgene, Fisher Scientific #09-740-106) and immediately used or stored at -80ºC for up to one month ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... the fibroblasts were thawed and plated at a density of 2.5×105 cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Cell Biology 2024Quote: We established mouse iPSC lines (CD1 iPSC or ntdTomato iPSC) using Sendai virus-mediated reprogramming kit: CytoTune2.0 (Thermo Fisher, A16517) and followed manufactured protocol as described before24 ...
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bioRxiv - Neuroscience 2024Quote: ... sections of virus-injected mice were incubated in a solution of chicken anti-eGFP (1:2000, Thermo Fisher Scientific, A10262) and mouse anti-gephyrin (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Biochemistry 2024Quote: ... Ten micrograms of total RNA was reverse transcribed (RT) at 37 °C using random hexamer primers and Moloney murine leukemia virus retrotranscriptase (Invitrogen-Life Technologies ...
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bioRxiv - Microbiology 2024Quote: Virus-infected cells and the supernatants were dissolved with Tris-glycine SDS sample buffer (Thermo Fisher Scientific, Waltham, MA, USA), boiled for 5 min in the absence of reducing agent ...
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bioRxiv - Cell Biology 2024Quote: ... were co-transfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using Lipofectamine 3000 (Cat# L3000001, ThermoFisher). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cell density was measured as an optical density (OD, 595 nm; Multiscan FC Thermo Scientific) and colony forming units per ml (CFU/ml ...
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bioRxiv - Microbiology 2020Quote: ... RBD-Fc was biotinylated with EZ‐link sulfo‐NHS‐LC‐biotin (Thermo Fisher Scientific, Waltham, MA) (RBD-Fc-Bio) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with final 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest, Nuaillé, France) and antibiotics (Gibco, Waltham, MA, USA). The human NSCLC cell line NCI-H23 (H23 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco). Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells ...
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bioRxiv - Biophysics 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 % fetal calf serum (FCS) (Biochrom, Ltd., Berlin, Germany) and 1 % penicillin-streptomycin (Invitrogen). The human colorectal adenocarcinoma cell line Caco-2 was provided by Jindrich Cinatl Jr ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were pre-incubated with Fc-Block (eBioscience, Thermo Fisher Scientific, Les Ulis, France) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and absorbance were subsequently measured at 450 nm using a Multiskan™ FC photometer (Thermo Fisher).
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with anti-CD16/32 (Fc Block) antibodies and Fixable Live/Dead stain (Invitrogen) for 15 min before staining with antibodies for surface markers in 2% BSA/PBS buffer for 30 mins on ice ...
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bioRxiv - Immunology 2019Quote: ... Cells were cultured in DMEM (Wisent) or RPMI1640 supplemented with 10% FCS and penicillin/streptomycin (Gibco). Media were supplemented with 200 UI/mL IL-2 (Proleukin – Novartis Pharmaceuticals ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% (v/v) heat-inactivated Hyclone fetal calf serum (FCS; Thermo Scientific, UT, USA), 2mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
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bioRxiv - Microbiology 2020Quote: COS7 and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... washed in TBS and blocked with StartingBlock T20 Blocking buffer containing Fc blocker (Thermo Scientific, 37543). Sections were incubated with unconjugated L ...
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... was measured as absorbance at 450 nm (OD450) by Spectrophotometer (Thermo Scientific, Multiskan FC Microplate Photometer). The titers were defined from the reciprocal value of the absorbance at OD450.
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Cells were then subjected to Fc blockade with anti-CD16/CD32 (Thermo Fisher Scientific, Waltham, MA), washed with 1XPBS ...
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bioRxiv - Immunology 2022Quote: ... explanted organs were stored in 600 μL DMEM containing 10% FCS and 1% Penicillin/Streptomycin (Gibco). After mechanical disruption by shaking at 25/s over 4 min with metal beads (TissueLyser II ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% (vol/vol) fetal calf serum (FCS, Biochrom) and antibiotics (1x Pen/Strep, Gibco). tRF were generated from primary rhesus fibroblasts (RF ...
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bioRxiv - Immunology 2023Quote: ... The IgGs were purified by adding Protein G (Cytiva) or CaptureSelect IgG-Fc resin (Thermo Scientific) to the supernatant and rolling overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were cultivated in DMEM-F12 medium containing 20% fetal bovine serum (FCS, Thermo Fisher Scientific ...