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Citations for Thermo Fisher :
2701 - 2750 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... plasmids were cleaved with FD SgsI (Thermo Scientific™) and treated with FastAP termosensitive alkaline phosphatase (Thermo Scientific™) ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids and PEI were diluted in Opti-MEM (Gibco) at a 1µg:5µg DNA:PEI ratio ...
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bioRxiv - Genetics 2023Quote: ... or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). Plasmids were digested with restriction enzymes from NEB and ligated using T4 DNA ligase (NEB M0202) ...
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bioRxiv - Biophysics 2023Quote: ... modified pFastBac1 plasmids using T4 DNA Ligase (Thermo Scientific) supplemented with 0.2 µL FastDigest EheI (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: Plasmids were amplified using E.Coli DH5α strain (Life Technologies). DNA was then purified using the NucleoBond® Xtra Plus Midi-Prep kit (Machery-Nagel®) ...
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bioRxiv - Microbiology 2023Quote: ... FastDigest SfiI digested plasmid pSBbi-bla (Thermo Fisher Scientific), dephosphorylated using FastAP Thermosensitive Alkaline Phosphatase (1 U/µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with plasmids using Lipofectamine 3000 (Invitrogen) as described by the manufacturer.
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bioRxiv - Neuroscience 2023Quote: ... Neurons were transfected with plasmids using Lipofectamine 2000 (Invitrogen) at 10-11 DIV.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The plasmid constructs were synthetized by GENEART (ThermoFisher Scientific) and designed with the wildtype CFTR intron 5-6 (880nt ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were expressed using an Expi293F (GIBCO, cat#100044202) cell-based expression system and RBD proteins were purified using Protein A affinity resin (Genscript Cat# ...
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bioRxiv - Microbiology 2023Quote: ... and transformed plasmids into DH5α-competent cells (Thermo Fisher). We sequenced plasmids and confirmed mutation after purification with plasmid purification (Qiagen) ...
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bioRxiv - Systems Biology 2023Quote: ... was used for plasmid transfection and Lipofectamine RNAiMAX (Invitrogen) was used for β-catenin siRNA experiment ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids were transfected into Expi293F cells (Thermo Fisher Scientific) in a 1:1 ratio of heavy chain:light chain using ExpiFectamine according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid transfections were performed with Opti-MEM (Thermo Fisher) mixed with sequence-verified plasmid DNA and PEI MAX transfection reagent (Polysciences ...
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bioRxiv - Biochemistry 2022Quote: ... After plasmid transformation in Escherichia coli (BL21 DE3, Invitrogen), bacteria were grown in 3-6 liter of LB medium containing 0.1 mg.ml-1 of appropriate antibiotics (ampicillin or kanamycin ...
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bioRxiv - Cell Biology 2023Quote: Plasmids were constructed using multi-site Gateway system (Thermofisher). Promoters used in this study include ...
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were linearized with SwaI (ThermoFisher, cat. no. FD1244). Capped mRNA was synthesized with T7 polymerase (Ambion mMessage mMachine T7 kit ...
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bioRxiv - Microbiology 2023Quote: ... and DSP1-7 plasmids using Lipofectamine 3000 (Thermo Scientific) according to manufacturer’s instructions in triplicates ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... cells were transfected with plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligated plasmids were transformed into DH5α E.coli (Life Technologies). The PX330-Alk-Eml4 plasmid was kindly provided by Andrea Ventura [26].
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bioRxiv - Developmental Biology 2023Quote: ... Purified linear plasmid was transfected using Lipofectamine 3000 (Invitrogen). Stably expressing mCherry-positive XEN cells were flow sorted ...
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bioRxiv - Molecular Biology 2023Quote: All plasmids were transfected using Lipofectamine 2000 (11668019, Invitrogen), and siRNA were transfected by Lipofectamine RNAiMax (13778150 ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... Plasmids were purified with the GeneJet miniprep (ThermoFisher #K0503) and verified via Sanger sequencing (Genewiz ...
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bioRxiv - Genomics 2023Quote: ... 500 ng plasmid was transfected using Lipofectamine 2000 (Invitrogen) according to the manufacture instruction ...
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bioRxiv - Cell Biology 2022Quote: ... and plasmids were purified via GeneJet miniprep (ThermoFisher #K0503). The transposase plasmid pCMV(CAT)T7-SB100 was a gift from Zsuzsanna Izsvak (Addgene #34879) ...
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bioRxiv - Microbiology 2023Quote: ... and transformed plasmids into DH5α-competent cells (Thermo Fisher). We sequenced plasmids and confirmed mutations after purification with plasmid purification (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: ... The plasmids were recombined using LR Clonase II (Invitrogen) following the manufactures instructions ...
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bioRxiv - Microbiology 2023Quote: ... extracted using NucleoSpin Plasmid QuickPure Kit (Thermo Fisher Scientific) and confirmed by sequencing at Plasmidsaurus (USA) ...
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bioRxiv - Systems Biology 2024Quote: ... or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). Plasmids were digested with restriction enzymes from New England Biolabs (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... 500 ng plasmid and 1 μL Lipofectamine 2000 (Invitrogen) reagent were mixed in Opti-MEM medium and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected into AGS via Lipofectamine 2000 (ThermoFisher). siRNA (siNFE2L3#1 ...
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bioRxiv - Cancer Biology 2023Quote: ... or PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen; # K210007), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfections were performed using Lipofectamine 2000 (ThermoFisher, 11668019) or Calcium Phosphate (Promega ...
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bioRxiv - Microbiology 2024Quote: ... parental plasmids were digested with DpnI (Thermo Fisher Scientific) and the linear PCR product was transformed into chemically competent MG1655 cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were transformed into ElectroMAX DH10B Cells (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid-based transfection was performed with Lipofectamine 3000 (Invitrogen) for U251 cells ...
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bioRxiv - Biophysics 2024Quote: ... and extracted by GeneJET plasmid miniprep kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: Plasmids were transfected into cells using Lipofectamine 3000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids are maintained in the bacterial strains: DH5α (ThermoFisher) for standard plasmids ...
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bioRxiv - Bioengineering 2024Quote: ... Plasmids were transiently transfected into Expi293 cells (ThermoFisher Scientific). After 5 days ...
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bioRxiv - Synthetic Biology 2024Quote: ... with vacuum manifold and GeneJET Plasmid Midiprep Kit (ThermoFisher) were used for higher yields.
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bioRxiv - Microbiology 2024Quote: ... The linearized plasmids were also dephosphorylated with FastAP (ThermoFisher). The products of the HiFi reaction were transformed into NEB10β competent cells (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Ligated plasmids were transformed into DH5α bacteria (Thermo Fisher) and grown overnight on LB plates containing carbenicillin to select for colonies that successfully received the plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNAs were forward-transfected Lipofectamine 3000 (Invitrogen, #L3000001) and following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2021Quote: ... The following secondary antibodies were used: goat α-rat Alexa Fluor 647 (1:1000; A21247; Invitrogen), goat α-rabbit Alexa Fluor 568 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies goat anti-rat Alexa Fluor 488 and goat anti rabbit Alexa Fluor 647 (Invitrogen). Sections were counterstained with SYTOX-Green at 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... CD11b+ microglia and macrophages were identified using rat anti-CD11b antibodies (ThermoFisher, cat#14-0112-82). GFAP was stained using rabbit anti-GFAP antibodies (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were then incubated with Alexa Fluor 594 goat anti-rat antibody (Thermo Fisher; 1:100) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and rat and Alexa Fluor 532 conjugated to antibodies raised against mouse and rabbit (Invitrogen) were used at 1/200 dilution in the saturation media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200, Invitrogen). For co-localization experiments with ER or mitosomal markers ...